LOCUS       sequence-281495-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1462
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1075..1083
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggatatagc aacaggtccc gagtcgctgg agaggtgctt ccctcgcggg
       61 cagacggact gcgccaagat gttggacggc atcaagatgg aggagcacgc cctgcgcccc
      121 gggcccgcca ctctgggggt gctgctgggc tccgactgcc cgcatcccgc cgtctgcgag
      181 ggctgccagc ggcccatctc cgaccgcttc ctgatgcgag tcaacgagtc gtcctggcac
      241 gaggagtgtt tgcagtgcgc ggcgtgtcag caagccctca ccaccagctg ctacttccgg
      301 gatcggaaac tgtactgcaa acaagactac caacagctct tcgcggccaa gtgcagcggc
      361 tgcatggaga agatcgcccc caccgagttc gtgatgcggg cgctggagtg cgtgtaccac
      421 ctgggctgct tctgctgctg cgtgtgtgaa cggcagctac gcaagggcga cgaattcgtg
      481 ctcaaggagg gccagctgct gtgcaagggt gactacgaga aggagaagga cctgctcagc
      541 tccgtgagcc ccgacgagtc cgactccgtg aagagcgagg atgaagatgg ggacatgaag
      601 ccggccaagg ggcagggcag tcagagcaag ggcagcgggg atgacgggaa ggacccgcgg
      661 aggcccaagc gaccccggac catcctcacc acgcagcagc gaagagcctt caaggcctcc
      721 ttcgaggtct cgtcgaagcc ttgccgaaag gtccgagaga cactggcagc tgagacgggc
      781 ctcagtgtgc gcgtggtcca ggtctggttt cagaaccaaa gagcaaagat gaagaagctg
      841 gcgcggcggc accagcagca gcaggagcag cagaactccc agcggctggg ccaggaggtc
      901 ctgtccagcc gcatggaggg catgatggct tcctacacgc cgctggcccc accacagcag
      961 cagatcgtgg ccatggaaca gagcccctac ggcagcagcg accccttcca gcagggcctc
     1021 acgccgcccc aaatgccagg gaacgactcc atcttccatg acatcgacag cgatacctcc
     1081 ttaaccagcc tcagcgactg cttcctcggc tcctcagacg tgggctccct gcaggcccgc
     1141 gtggggaacc ccatcgaccg gctctactcc atgcagagtt cctacttcgc ctcctagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgacaaat gatatccgcg taaagggcc
//
