LOCUS       sequence-281624-        1646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1841
FEATURES             Location/Qualifiers
     source          1..1646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgtggga agtgaagaca aatcagatgc ctaatgcagt acagaaactc
       61 ctgttggtga tggacaagag agcctcagga atgaatgact cattggagtt gctgcagtgt
      121 aatgagaatt tgccatcttc acctggatat aactcctgtg atgaacacat ggagcttgat
      181 gaccttcctg aacttcaggc agttcaaagt gatcctaccc aatctggcat gtaccagctg
      241 agttcagatg tttcacatca agaataccca agatcatctt ggaaccaaaa tacctcagac
      301 ataccagaaa ctacttaccg tgaaaatgag gtggactggc taacagaatt ggcaaatatc
      361 gcgaccagtc cacaaagtcc actgatgcag tgctcatttt acaatagatc atctcctgta
      421 cacatcatag ccactagcaa aagtttacat tcctatgcac gccctccacc agtgtcctct
      481 tcttcgaaga gtgaaccagc cttccctcat caccattgga aggaggaaac accagtaaga
      541 cacgaaaggg caaatagtga gtcagaatct ggcattttct gcatgtcctc cctgtcagat
      601 gatgatgatt tgggatggtg caattcctgg ccttcaactg tctggcactg ttttttgaaa
      661 ggcacacgac tgtgctttca taagggaagc aataaggaat ggcaagatgt tgaagatttt
      721 gctagagctg aaggctgtga taatgaggaa gatcttcaaa tgggcattca caagggctat
      781 ggttctgatg gtctaaagtt gttatcacat gaagaaagtg tatcatttgg cgagtctgta
      841 ctgaagttga cttttgatcc tggtacagta gaagatggtt tacttaccgt agagtgtaag
      901 ctggaccacc ctttctatgt taaaaataaa ggttggtcat cattttatcc aagcttgact
      961 gtggtacagc atggcattcc atgttgtgaa gttcatattg gcgatgtatg tctacctcct
     1021 ggacaccccg atgccattaa ttttgatgat tcaggtgttt ttgatacatt taaaagctat
     1081 gacttcacac ctatggattc ttctgcagtt tatgtgttaa gtagtatggc tcgccagcgt
     1141 cgtgcatctt tgtcttgtgg aggacctggt ggtcaagact ttgcaagatc tggattcagt
     1201 aaaaactgtg gctcacctgg atcatcacag ctctcttcca attctttgta tgctaaagct
     1261 gtcaaaaacc acagctcagg gactgtgagt gccacttctc ctaataagtg caaaagacca
     1321 atgaatgcct tcatgctttt tgccaaaaaa tacagagttg aatatactca gatgtatcca
     1381 gggaaagata acagagccat aagtgtgatc cttggtgaca ggtggaagaa aatgaagaat
     1441 gaagagagaa gaatgtacac attagaagca aaggctttgg ctgaagaaca gaaacgttta
     1501 aatcctgact gttggaagag gaaaagaacc aattcaggct cacaacaaca ttagtaatga
     1561 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1621 gacagatgaa tagggtgatc ggactt
//
