LOCUS       sequence-281825-        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0610
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtggat taaaaggaaa tttggtgagc ggcctccacc taaacgactt
       61 actagggaag ctatgcgaaa ttatttaaaa gagcgagggg atcaaacagt acttattctt
      121 catgcaaaag ttgcacagaa gtcatatgga aatgaaaaaa ggtttttttg cccacctcct
      181 tgtgtatatc ttatgggcag tggatggaag aaaaaaaaag aacaaatgga acgcgatggt
      241 tgttctgaac aagagtctca accgtgtgca tttattggga taggaaatag tgaccaagaa
      301 atgcagcagc taaacttgga aggaaagaac tattgcacag ccaaaacatt gtatatatct
      361 gactcagaca agcgaaagca cttcatgttg tctgtaaaga tgttctatgg caacagtgat
      421 gacattggtg tgttcctcag caagcggata aaagtcatct ccaaaccttc caaaaagaag
      481 cagtcattga aaaatgctga cttatgcatt gcctcaggaa caaaggtggc tctgtttaat
      541 cgactacgat cccagacagt tagtaccaga tacttgcatg tagaaggagg taattttcat
      601 gccagttcac agcagtgggg agcctttttt attcatctct tggatgatga tgaatcagaa
      661 ggagaagaat tcacagtccg agatggctac atccattatg gacaaacagt caaacttgtg
      721 tgctcagtta ctggcatggc actcccaaga ttgataatta ggaaagttga taagcagacc
      781 gcattattgg atgcagatga tcctgtgtca caactccata aatgtgcatt ttaccttaag
      841 gatacagaaa gaatgtattt gtgcctttct caagaaagaa taattcaatt tcaggccact
      901 ccatgtccaa aagaaccaaa taaagagatg ataaatgatg gcgcttcctg gacaatcatt
      961 agcacagata aggcagagta tacattttat gagggaatgg gccctgtcct tgccccagtc
     1021 actcctgtgc ctgtggtaga gagccttcag ttgaatggcg gtggggacgt agcaatgctt
     1081 gaacttacag gacagaattt cactccaaat ttacgagtgt ggtttgggga tgtagaagct
     1141 gaaactatgt acaggtgtgg agagagtatg ctctgtgtcg tcccagacat ttctgcattc
     1201 cgagaaggtt ggagatgggt ccggcaacca gtccaggttc cagtaacttt ggtccgaaat
     1261 gatggaatca tttattccac cagccttacc tttacctaca caccagaacc agggccgcgg
     1321 ccacattgca gtgcagcagg agcaatcctt cgagccaatt caagccaggt gccccctaac
     1381 gaatcaaaca caaacagcga gggaagttac acaaacgcca gcacaaattc aaccagtgtc
     1441 acatcatcta cagccacagt ggtatcctag taatgaacta gtccgtaact tgaaagtatt
     1501 tcgatttctt ggctttatat atcttgtgga aaggacgagg gcgactcgtc tggtaccaat
     1561 gt
//
