LOCUS       sequence-28215-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Maida Y, Yasukawa M, Furuuchi M, Lassmann T, Possemato R, Okamoto N,
            Kasim V, Hayashizaki Y, Hahn WC, Masutomi K
  TITLE     An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA.
  JOURNAL   Nature. 2009 Sep 10. 461(7261):230-5.
  PUBMED    19701182
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 10. 461(7261):230-5."
COMMENT     Sequence Label: pWZL-Blast-Flag-HA-hTERT
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..203
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     219..235
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             280..303
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             316..342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             316..342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aacgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
       61 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
      121 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
      181 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
      241 ctctaggcgc cggccggatc ttccgctcga tgaaccatgg actacaagga cgacgatgac
      301 aagctcgatg gaggataccc ctacgacgtg cccgactacg ccggaggact cgaggaattc
      361 gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc gcgcgctccc
      421 cgctgccgag ccgtgcgctc cctgctgcgc aaccactacc gcgaggtgct gccgctggcc
      481 acgttcgtgc ggctcctggg gccccagggc tggcggctgg tgcagcgcgg ggacccggcg
      541 gctttccgcg cgctggtggc ccagtgcctg gtgtgcgcgc cctgggacgc acggccgccc
      601 cccgccgccc cctccttccg ccagtngtcc tgcctgaagg agctggtggc ccgagtgctg
      661 canaggctgt tcgagcgcgg cgcgaataac gtgctgngcc tt
//
