LOCUS       sequence-282233-        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2376
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagga ctggctggac tgcccggccc tgggccctgg ctggaagcgc
       61 cgcgaagtct ttcgcaagtc aggggccacc tgtggacgct cagacaccta ttaccagagc
      121 cccacaggag acaggatccg aagcaaagtt gagctgactc gatacctggg ccctgcgtgt
      181 gatctcaccc tcttcgactt caaacaaggc atcttgtgct atccagcccc caaggcccat
      241 cccgtggcgg ttgccagcaa gaagcgaaag aagccttcaa ggccagccaa gactcggaaa
      301 cgtcaggttg gaccccagag tggtgaggtc aggaaggagg ccccgaggga tgagaccaag
      361 gctgacactg acacagcccc agcttcattc cctgctcctg ggtgctgtga gaactgtgga
      421 atcagcttct caggggatgg cacccaaagg cagcggctca aaacgttgtg caaagactgt
      481 cgagcacaga gaattgcctt caaccgggaa cagagaatgt ttaagcgtgt gggctgtggg
      541 gagtgtgcag cctgccaggt aacagaagac tgtggggcct gctccacctg cctcctgcag
      601 ctgccccatg atgtggcatc ggggctgttc tgcaagtgtg aacggagacg ctgcctccgg
      661 attgtggaaa ggagccgagg gtgtggagta tgccggggct gtcagaccca agaggattgt
      721 ggccattgcc ccatctgcct tcgccctccc cgccctggtc tcaggcgcca gtggaaatgt
      781 gtccagcgac gttgcctacg gcaccttgct caccgcctgc gtcgccgtca tcagagatgt
      841 cagcgacgca ctcccctggc tgtggctccc ccaactggta aacatgcccg ccgcaaggga
      901 ggctgtgact ccaagatggc tgccaggcgg cgccccggag cccagccact gcctccacca
      961 cccccatcac agtccccaga gcccacagag ccgcacccca gagccctggc cccctcgcca
     1021 cctgccgagt tcatctatta ctgtgtagac gaggacgagc tacagcccta cacgaaccgc
     1081 cggcagaacc gcaagtgcgg ggcctgtgca gcctgcctac ggcggatgga ctgtggccgc
     1141 tgcgacttct gctgcgacaa gcccaaattc gggggcagca accagaagcg ccagaagtgt
     1201 cgttggcgcc aatgcctgca gtttgccatg aagcggctgc tgcccagtgt ctggtcagag
     1261 tctgaggatg gggcaggatc gcccccacct taccgtcgtc gaaagaggcc cagctctgcc
     1321 cgacggcacc atcttggccc taccttgaag cccaccttgg ctacacgcac agcccaacca
     1381 gaccataccc aggctccaac gaagcaggaa gcaggtggtg gctttgtgct gcccccgcct
     1441 ggcactgacc ttgtgttttt acgggaaggc gcaagcagtc ctgtgcaggt gccgggccct
     1501 gttgcagctt ccacagaagc cctgttgcag gaggcccagt gctctggcct gagttgggtt
     1561 gtggccttac cccaggtgaa gcaagagaag gcggataccc aggacgagtg gacaccaggc
     1621 acagctgtcc tgacttctcc cgtattggtg cctggctgcc ctagcaaggc agtagaccca
     1681 ggcctgcctt ctgtgaagca agagccacct gacccagagg aggacaagga ggagaacaag
     1741 gatgattctg cctccaaatt ggccccagag gaagaggcag gaggggctgg cacacccgtg
     1801 atcacggaga ttttcagcct gggtggaacc cgcttccgag atacagcagt ctggttgcca
     1861 aggtccaaag accttaaaaa acctggagct agaaagcagt agtaatgaac tagtccgtaa
     1921 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ttagactgac
     1981 tgtttggagc tgtc
//
