LOCUS       sequence-282311-        1868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0232
FEATURES             Location/Qualifiers
     source          1..1868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgcc ggcgttggca ttagtatcat ttgaagacgt ggttgtgacc
       61 ttcactggag aggaatgggg gcacctggac ctggcccaga ggaccctgta ccaggaggtg
      121 atgctggaga cctgcaggct cctggtctca ctggggcatc ctgttcccaa accagagctg
      181 atctatctac tggaacatgg acaggaactg tggacagtga agagaggcct ctcccaaagc
      241 acctgcgcag gtgaaaaagc aaaacccaag attacagagc ctactgcttc tcagctggcc
      301 ttctctgagg aatcctcttt ccaggaactt ctggcacaga gatcctcaag agattccagg
      361 ttggggcaag ctagagatga ggaaaagcta ataaaaattc aggaagggaa cttgaggcca
      421 ggaacaaacc cccacaaaga gatatgccct gagaagttga gttataaaca tgatgatttg
      481 gagccagatg atagtctggg cttaagggtt ttacaggaac gagtcactcc acaagatgct
      541 ctccatgagt gtgactctca aggaccagga aaagacccca tgactgatgc aaggaataac
      601 ccttacacat gcacggaatg tgggaaaggg tttagcaaga agtgggccct tgttcggcat
      661 caacagattc atgctggagt gaagccctat gagtgcaatg agtgtgggaa agcctgtcgt
      721 tatatggctg atgtcattcg acatatgagg cttcatactg gggaaaaacc atacaagtgt
      781 attgagtgtg ggaaagcctt caaacgcagg tttcacctca cggagcacca gcgtattcac
      841 accggagata agccctatga gtgcaaagaa tgtggcaaag cattcaccca ccgctcttct
      901 tttatccagc ataatatgac tcacactcga gaaaaaccct ttttatgcaa agaatgtggg
      961 aaagcttttt actacagctc ctcatttgct caacatatga ggattcatac tggaaagaaa
     1021 ctctatgagt gcggtgaatg tggaaaggcc ttcacgcacc gctccacatt tatccagcac
     1081 aatgtgaccc acacaggaga aaaaccattt ttatgtaaag aatgtggaaa aaccttttgc
     1141 ctcaactcat ccttcactca gcacatgagg attcacactg gagagaaacc ctatgagtgc
     1201 ggtgaatgtg gaaaggcctt tactcatcgc tccactttca tccgacataa gaggacccat
     1261 accggagaga agccctttga gtgcaaagaa tgtgggaagg ccttttgtga cagctcttcc
     1321 ttaattcaac atatgaggat tcacactggt gagaagcctt atgagtgcag tgaatgtgga
     1381 aaggctttta cacaccactc tgtttttatt cgacataata ggacccacag tggacaaaaa
     1441 cccttggagt gcaaagaatg tgcaaaagcc ttttactata gctcttcctt cactcgacac
     1501 atgaggattc acactggaga gaagccctat gtttgtagag aatgtggaaa ggcttttacc
     1561 caacctgcaa attttgttcg gcataatagg atccacactg gagaaaaacc ttttgaatgc
     1621 aaagaatgtg agaaagcctt ctgtgacaac tttgctttaa ctcagcacat gagaactcac
     1681 actggagaga aaccctttga atgcaatgaa tgtggaaaga ccttcagcca cagttcatcg
     1741 ttcactcacc atcgaaagat tcataccaga gtttagtaat gaactagtcc gtaacttgaa
     1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaacgtgc agtggctgcg
     1861 tggtgagt
//
