LOCUS       sequence-282425-        3287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0686
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3135..3152
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tgtctgctta ctctgtgcct tcaacttttg cccaggcctc attgccagtt
       61 cataaccagg tgctgccttc catcgagagt gtagatgggt ccgacccttt ggcaactctg
      121 cagacccctc taggcagact ggaggccaaa gaggaagagg atgaggatga ggacgaggac
      181 actgaggaag atgaggaaga agacggtgag gacacggatc tggatgactg ggagccagac
      241 ccgccccggc ccttcgaccc acacgacttg tggtgtgagg agtgcaataa cgcgcatgct
      301 tcagtgtgtc cgaagcacgg ccccttgcac ccgatcccca accggccggt gctcacccgg
      361 gccagggcga gcctccccct ggtgctctac atagacaggt ttctgggcgg ggtgttctcc
      421 aagcggcgca tccccaagcg cacccagttt ggccccgtgg aggggcctct cgtcaggggc
      481 tcggagctga aagactgtta cattcacctc aaggtttctc ttgataaagg ggacaggaaa
      541 gaaagggatt tacatgaaga cctatggttt gagttgtctg atgagacgct ttgtaactgg
      601 atgatgtttg tacggccagc ccagaatcac ctggagcaga acctggtggc ttaccagtat
      661 ggccaccatg tgtattatac aaccataaaa aatgtggagc ccaagcagga actgaaggtg
      721 tggtatgccg catcctatgc tgagttcgtg aaccagaaaa ttcatgacat ttctgaggaa
      781 gaaaggaaag ttcttcgaga gcaagagaag aattggccct gctatgaatg taaccgccga
      841 tttataagct cggagcagtt gcaacagcat ctcaattctc atgatgagaa actagatgtg
      901 tttagcagaa caagaggcag aggaagggga cgaggcaaga ggcgattcgg tccaggtcga
      961 cggccggggc gtcctccaaa atttatccgc ctggaaatca ccagcgaaaa tggggaaaag
     1021 agtgacgatg ggacacagga cttgctacat tttcccacaa aggagcaatt tgatgaggct
     1081 gaaccagcca ctctgaatgg gctggatcaa ccagaacaga ccactatccc aatccctcag
     1141 ctgccacagg aaacccagtc ttccctggaa catgaaccag aaactcacac cctgcacctg
     1201 cagccgcagc atgaagagag cgtggtgccc acccagagca cgctgacagc cgacgacatg
     1261 cgcagagcca agcgcatccg attggagctg cagaatgcag ctcttcagca tctgtttatt
     1321 cggaagtcct tccggccttt taaatgcttg cagtgtggga aggccttccg ggaaaaggac
     1381 aaactggacc agcacttacg cttccatggg cgggagggga actgcccact gacctgtgat
     1441 ctctgtaaca agggcttcat cagcagcaca tccttggaga gccacatgaa gctccactca
     1501 gaccagaaga cttactcttg cattttttgc ccagaatcct ttgaccgcct tgatttgttg
     1561 aaagatcatg tggccattca tatcaatgat ggctacttca cctgcccaac ttgtaagaaa
     1621 cggttcccag attttatcca ggtgaaaaaa cacgtgcgca gcttccactc agaaaagatc
     1681 taccagtgca cagagtgtga caaggccttc tgtcgccccg ataaactgcg actccacatg
     1741 ctccggcatt cggaccgcaa agacttcctg tgttccacct gtgggaagca atttaagcga
     1801 aaagacaaac tacgggaaca catgcagagg atgcataatc ctgagaggga ggccaagaaa
     1861 gccgaccgca tcagccgctc caagacgttc aagccccgca tcacgtccac agactacgac
     1921 agcttcacgt tcaagtgccg cctgtgcatg atgggcttcc ggcggcgcgg catgctggta
     1981 aatcacttat cgaagagaca cccagacatg aagatagaag aggtgccaga gttaactcta
     2041 cccatcataa aacccaatcg tgattacttt tgtcagtatt gcgataaggt ttataaaagt
     2101 gccagcaagc gcaaagccca cattctgaag aaccacccag gagcagagct cccaccgagc
     2161 attcggaagc tccgacccgc tggtcctgga gagccagacc ccatgctgag cacacacacc
     2221 cagctgacgg gcaccatcgc cacccctccc gtctgctgtc cccactgctc caagcagtac
     2281 agcagcaaga ccaagatggt ccagcacatt cgaaagaagc atccagagtt cgcccagctc
     2341 tccaacacca tacacacacc actgacgaca gctgtgatca gtgccacccc agcggttttg
     2401 actacagaca gcgccactgg agagactgtg gtgacgacgg acctgctcac ccaagcaatg
     2461 acagaactgt cccagacctt aacgacagac taccgaacgc cacaagggga ttaccagaga
     2521 attcagtaca tccctgtgtc gcagtcggcg tctggcctcc agcagcctca gcacatacag
     2581 ctgcaagtgg ttcaagtggc ctcggccact tcccctcacc agtcacagca gtccactgtg
     2641 gatgttggcc agctccatga tcctcagccc tacccccagc acgccatcca ggtgcagcac
     2701 atccaggtat ctgggcagcc gttgagtccc tcagcccagc aggctcagca ggggctcagc
     2761 ccctcccaca tccagggcag ttcttccaca caggggcagg ctctgcagca gcagcagcag
     2821 cagcagcaga attcctctgt gcagcacacg tacctgccca gtgcttggaa ttccttccgt
     2881 ggctattcat ctgagattca aatgatgacg cttcctccgg gtcagtttgt gattacagac
     2941 agtggtgtgg caactccagt tactactggc caggtgaagg cggttacttc gggtcattat
     3001 gtgttatcag aaagtcaatc agaattggaa gaaaagcaaa cttctgccct ctctggtgga
     3061 gtccaggtcg agccacctgc acacagtgac tccctggacc cccagaccaa cagccaacag
     3121 cagaccacac agtacatcat caccaccacc accaacggga acggaagcag cgaagtgcat
     3181 atcaccaaac catagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3241 tatatatctt gtggaaagga cgagcactta atcaatccac gtcaaga
//
