LOCUS       sequence-282440-        2861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0728
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgccga cgtcttcatg tgctccccgc gccggcctcg cagccggggc
       61 cgccaggtgc tgctcaagcc ccaggtgtcc gaggacgacg acgactcgga cacggatgag
      121 ccgtccccgc cgcccgcctc cggcgcggcc accccggccc gggcccacgc gagcgccgcg
      181 ccaccgccgc ctcgggccgg gccgggccgc gaggaacctc cgcgccgcca gcagatcatc
      241 cacagcggcc acttcatggt gtcgtcgccg caccgagagc acccgcccaa gaagggctac
      301 gatttcgaca cggtcaacaa acagacgtgc cagacctaca gcttcggcaa gactagctcc
      361 tgccacctgt ccatcgacgc ctcgctcacc aagctcttcg agtgcatgac tttggcctac
      421 agtgggaagt tggtgtctcc aaagtggaag aatttcaagg gcctgaagct acagtggaga
      481 gacaagatcc ggctcaataa tgccatctgg cgggcctggt acatgcagta tctggagaag
      541 cgcaagaatc ctgtgtgcca ctttgtgaca cccctggacg gctctgtgga cgtagacgag
      601 caccgccggc cggaggccat caccacggaa gggaagtact ggaagagccg catcgagatt
      661 gtgatccggg agtatcacaa gtggagaacc tacttcaaga aaaggctaca gcagcacaag
      721 gatgaggacc tctccagcct ggtccaggac gatgacatgc tgtattggca caagcacggg
      781 gatggatgga agacccccgt ccccatggag gaggatcccc tgctggacac agacatgctc
      841 atgtcggaat tcagcgacac cctcttctcc acactttctt cacaccagcc ggtggcctgg
      901 cccaatcccc gggaaatagc acatctggga aatgcagaca tgatccagcc gggactgatt
      961 cctttgcagc ctaacctgga cttcatggac acctttgagc ctttccagga cctcttctct
     1021 tctagccgct ccatttttgg ctccatgcta cctgcatctg cctcagcacc tgtaccagat
     1081 cccaacaacc cacctgcaca ggagagcatc ctgccgacca cagccctccc cactgtgagc
     1141 cttcctgaca gcctcatcgc gccccctacc gccccatccc tggctcacat ggatgagcag
     1201 ggctgtgaac acacctcccg gactgaggac ccgtttatcc agcccacgga cttcggtccc
     1261 tcagagccgc cactgagtgt cccgcagccc ttcctccctg tcttcaccat gcccctgctg
     1321 tctcccagcc ccgccccacc gcccatctcc cccgtgttac cattagttcc tcctcctgcc
     1381 actgccctga accccccggc tccacccacc ttccatcagc cacagaagtt tgctggagtc
     1441 aacaaagcgc cgtctgtcat cacccacacg gcctctgcca ccctcaccca cgatgccccc
     1501 gccaccacct ttagccagag tcagggcctt gtgatcacca cccatcaccc tgccccgtca
     1561 gcggcccctt gtgggctggc actgtctcct gtcacccggc ctccccagcc acggttaact
     1621 tttgtgcacc ccaaacctgt atccttgact gggggcaggc ctaagcagcc ccacaaaata
     1681 gtgcctgctc ccaaaccaga gcccgtgtcc ttggtgttga agaatgcccg tatcgcccca
     1741 gctgcctttt caggccaacc acaagcggtg atcatgacgt cagggcctct gaagagagaa
     1801 gggatgttgg cctccaccgt gtcccagtcc aacgtggtca ttgcgcctgc tgccatcgcc
     1861 agggctcctg gggtcccgga gttccacagc agcatcctgg tgacagatct cggccatggc
     1921 acgagcagcc cgcctgcccc cgtctcccgg ctcttcccaa gcacagcgca agaccccctg
     1981 gggaagggcg agcaggtccc gctgcatggg ggcagccccc aggtcactgt cacagggccc
     2041 agtcgggact gcccaaactc agggcaggcc tctccgtgtg catcggagca gagccccagt
     2101 cctcaatctc cccagaacaa ctgctcaggg aaatccgacc ccaaaaatgt ggctgcacta
     2161 aagaaccggc agatgaagca catctcagct gagcagaaaa ggcgcttcaa catcaagatg
     2221 tgcttcgaca tgctcaacag cctcatctcc aacaattcca agctgaccag tcacgccatc
     2281 acactgcaga agactgtgga gtacatcacc aagctgcagc aggagagagg ccagatgcag
     2341 gaggaggccc ggcggctgcg ggaggagatc gaggagctca atgccaccat catctcctgc
     2401 cagcagctgc tccctgccac gggagtcccc gttacccggc gccagtttga tcacatgaaa
     2461 gacatgtttg acgaatacgt gaaaacccgg accttgcaga attggaagtt ctggattttc
     2521 agcatcatca tcaagccgct gtttgagtcg ttcaagggca tggtgtccac cagcagcctg
     2581 gaggagctgc accggacggc gctctcctgg ctggaccagc actgctccct gcccatcctc
     2641 aggccgatgg tattgagcac gctgcggcag ctgagcacct ccacctccat cctcacagac
     2701 ccggcacagc tgccagagca ggcgtccaag gctgtcacca ggattggcaa gagattggga
     2761 gagtcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2821 tcttgtggaa aggacgacgt taccacttac ggtttcgtca g
//
