LOCUS       sequence-282458-        2696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0791
FEATURES             Location/Qualifiers
     source          1..2696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggatcgtt gacattttgg gatgtgacca tagaattcgc tctggaggag
       61 tggcaatgcc tggacatggc tcagcagaat ttatatagga atgttatgtt agagaactac
      121 agaaacctgg tcttcctggg tatcgctgtc tctaagctag acttgataac ttgtctgaag
      181 caagggaaag agccttggaa tatgaagaga catgagatgg taactaaacc cccagttatt
      241 agttctcatt ttacacaaga cttttggcca gatcagagca taaaagattc tttccaagaa
      301 ataatattga gaacatatgc aagatgtgga cataagaatt tacgattaag aaaagattgt
      361 gaaagtgtca atgagggtaa gatgcacgaa gaagcttata ataaacttaa ccaatgttgg
      421 acaactaccc agggaaaaat atttcagtgt aacaaatatg tgaaagtctt tcataaatat
      481 tcaaattcaa atagatataa gattagacac actaaaaaga aaactttcaa atgtatgaaa
      541 tgtagcaaat catttttcat gctttcacac ttaattcaac ataagagaat tcatactaga
      601 gagaatatct acaaatgtga agaacgtggc aaagccttta aatggttctc aacccttatt
      661 aaacataaga taattcatac tgaagacaaa ccctacaaat ataagaaatg tggcaaagct
      721 tttaacatct cttcaatgtt cactaaatgt aagataattc atactggaaa gaaaccctgc
      781 aaatgtgaag aatgtggcaa agtttttaac aattcctcaa cccttatgaa acataagata
      841 attcatactg ggaagaaacc atataaatgt gaagaatgtg gcaaagcttt taagcaatcc
      901 tcacacctta ctagacataa agcaattcat actggagaga aaccctacaa atgcgaagaa
      961 tgtggcaaag cttttaacca tttctcagcc cttagaaaac atcagataat tcatactgga
     1021 aagaaaccct acaaatgtga agaatgtggc aaagctttta gccagtcctc aacccttaga
     1081 aaacatgaga taattcatac tgaagagaaa ccctacaaat atgaagaatg cggcaaagct
     1141 tttagcaatt tgtcagccct tagaaaacat gagataattc atactggaca gaaaccctac
     1201 aaatgtgaag aatgtggtaa agcttttaag tggtcctcaa aacttactgt acataaggta
     1261 attcatactg cagagaaacc ctgcaaatgt gaagaatgtg gcaaagcttt taagcgtttc
     1321 tcagccctta gaaaacataa gataattcat actggaaagc aaccctacaa atgtgaagaa
     1381 tgcagcaaag cttttagcaa tttttcagcc cttagaaaac atgagataat tcatactgga
     1441 gagaaaccct acaaatgtga agaatgtggt aaagctttta agtggtcctc aaaacttact
     1501 gtacataagg taattcatat ggaagagaaa ccttgcaaat gtgaagaatg tggcaaagct
     1561 tttaagcatt tctcagccct tagaaaacat aagataattc atactggaaa gaaaccctac
     1621 aaatgtgaag aatgtggcaa agcttttaac aattcctcaa cccttatgaa acataagata
     1681 attcatactg ggaagaaacc atacaaatgt gaagaatgtg gcaaagcttt taagcaatct
     1741 tcacatctta ctagacataa agcaattcat actggggaga aaccctacaa atgtgaagaa
     1801 tgtggcaaag cttttaacca cttctcagcc cttagaaaac atcagataat tcatactgga
     1861 aagaaaccct acaaatgtga agaatgtggc aaagctttta gccagtcctc aacccttaga
     1921 aaacatgaga taattcatac tggagagaaa ccctacaaat gtgaagaatg tggtaaagct
     1981 tttaagtggt cctcacacct tactagacat aaagtaattc atactgaaga gaaaccctac
     2041 aaatgtgaag aatgtggcaa ggcttttaac catttctcag cccttaggaa acataagata
     2101 attcatactg gaaagaaacc ctacaaatgt gaagaatgtg gcaaagcttt tagccagtcc
     2161 tcaactctta gaaaacatga gataattcat actggagaga aaccctacaa atgtgaagaa
     2221 tgtggtaaag cttttaagtg gtcctcaaaa cttactgtac ataaggtaat tcatactgca
     2281 gagaaaccct gcaaatgtga agaatgtggc aaagctttta agcatttctc agcccttaga
     2341 aaacataaga taattcatac tggaaagaaa ccctataaat gtgaagaatg tggcaaagct
     2401 tttaacaatt cctcaaccct tagaaaacat gagataattc atactggaga gaaatcctac
     2461 aaatgtgaag aatgtggtaa agcttttcag tggtcctcaa aacttacttt acataaggta
     2521 attcatatgg agagaaaccc tgcaaatgtg aagaatgtgg caaagctttt aaacatttct
     2581 cagcccttag aaaacatgag atagtaatga actagtccgt aacttgaaag tatttcgatt
     2641 tcttggcttt atatatcttg tggaaaggac gagctcacgg tctatccgtg gaaaca
//
