LOCUS       sequence-282558-        2033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1179
FEATURES             Location/Qualifiers
     source          1..2033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctcagt ggctttagag gatgtggctg tgaacttcac ccgagaagag
       61 tgggctttgc tgggtccttg tcagaagaat ctctacaaag atgtgatgca ggaaaccatc
      121 aggaacctgg attgtgtagg aatgaaatgg aaagaccaga acattgaaga tcaatataga
      181 tatcccagga aaaatctaag atgtcgtatg ttagagagat ttgttgaaag taaagatgga
      241 actcaatgtg gagaaacatc tagccagatt caagatagta ttgtgaccaa gaacactctt
      301 cctggagtag gtccatatga aagccgtatg agtggagaag tcatcatggg tcattcatcc
      361 cttaattgtt acatcagagt tggtgctggg cacaaaccat atgagtatca tgaatgtgga
      421 gagaagccag atacgcataa acaacgtggg aaagccttca gttaccacaa ctcacttcaa
      481 acacatgaga ggcttcacac tggaaagaaa ccatataatt gtaaagaatg tgggaagtcc
      541 ttcagttctt tgggaaacct tcaaagacac atggcagtgc agcgtggaga tggaccttat
      601 aaatgtaagt tgtgtgggaa agcgtttttt tggcccagtt tattacatat gcatgagaga
      661 acgcacactg gagagaaacc atatgaatgt aagcagtgtt ctaaagcctt ttctttttac
      721 agttcctatc taagacatga aagaacacat actggggaga aactgtatga atgtaaacag
      781 tgttctaaag ccttccctga ttacagttct tgtctaagac atgaaagaac tcacactgga
      841 aagaaaccct atacatgtaa acaatgtggg aaagccttca gtgcttccac ttcccttcga
      901 agacacgaaa caactcacac tgatgagaaa ccctatgcat gtcagcaatg tgggaaagcg
      961 tttcatcatc tgggaagctt tcaaagacac atggtaatgc acacgagaga tggacctcat
     1021 aagtgtaaga tatgtggaaa aggctttgat tgtcctagtt cactgaaaag tcatgaaaga
     1081 actcacactg gagagaaact ctatgaatgc aagcagtgtg ggaaagcgtt atctcatagc
     1141 tcaagctttc gaagacacat gacaatgcac actggagatg gacctcacaa atgcaagata
     1201 tgtgggaaag cctttgttta tcccagtgta tttcaaaggc atgaaaagac tcacactgca
     1261 gagaaaccct ataaatgtaa acaatgtggc aaagcctacc gtatttccag ttctcttcga
     1321 aggcatgaaa caactcatac tggagagaaa ccctataaat gcaaatgtgg gaaagccttt
     1381 attgatttct attcctttca aaatcacaaa acaactcatg ctggagagaa gccatatgag
     1441 tgtaaggaat gtgggaaagc attcagttgt ttccaatacc tttctcaaca tagaaggact
     1501 cacacaggag agaaacctta tgagtgtaac acatgtaaga aagccttcag tcattttggt
     1561 aacttaaaag tacatgaaag aattcactct ggagagaagc cgtatgaatg taaggaatgt
     1621 gggaaagcat tctcttggct cacttgcttt ctacgacatg aaagaattca catgagagag
     1681 aaaccctatg agtgtcaaca atgtggtaaa gccttcactc attcccgttt tcttcaagga
     1741 catgaaaaaa ctcatactgg agagaacccg tatgaatgta aggaatgtgg gaaagcattt
     1801 gcttctctca gttccttgca tagacataaa aagactcact ggaaaaaaac tcacactgga
     1861 gagaacccgt atggatgtaa ggaatgtggg aaagcatttg cttctctcag ttccttgcat
     1921 agacataaaa agactcacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1981 tggctttata tatcttgtgg aaaggacgac atctcctgac cggatgagtt caa
//
