LOCUS       sequence-282579-        1559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1196
FEATURES             Location/Qualifiers
     source          1..1559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1195..1203
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgaaaaatgc cacgattgta atgtctgtga ggagagagca aggcagttct
       61 tcaggggagg ggtcattgtc attcgaagat gtggctgtgg gcttcaccag ggaggagtgg
      121 cagtttttgg accagtctca gaaggtcttg tacaaggaag taatgttgga gaactacatc
      181 aacctagtat caatagggta tcgaggcacc aagccagatt cgctcttcaa gttggagcaa
      241 ggagaacccc cagggatagc agaaggagca gcccacagtc aaatctgtcc aggttttgta
      301 atccagagta gaagatatgc aggaaaagat tctgatgcat ttggtggata tgggagatca
      361 tgcctccata tcaagcgtga caaaactctt actggagtta aataccatag atgtgttaaa
      421 cccagcagcc ctaaatcaca gctcaatgac ctacaaaaaa tttgtgcagg agggaaacca
      481 catgaatgca gtgtgtgcgg gagagccttc tccaggaaag cacagcttat tcaacatcag
      541 agaactgaaa gaggagagaa accccatgga tgtggtgaat gtgggaaaac attcatgagg
      601 aagattcagc tcactgagca tcagagaact cacacaggag agaagcccca tgaatgtagt
      661 gaatgtggaa aagccttctc cagaaagtca cagctcatgg tccatcagag aacccataca
      721 ggagagaaac cctacagatg cagcaaatgc ggaaaagcct tcagccggaa gtgccggctc
      781 aatagacatc agcgatcaca tactggagag aaactctatg ggtgcagtgt gtgtgggaaa
      841 gccttttctc agaaggcata cctcactgca caccagagac ttcacacagg agataagcct
      901 tataaatgca gtgattgtgg aagaaccttc tattttaagt cagacctgac cagacatcag
      961 aggattcata cgggagagaa accttatgaa tgtagtgagt gtgaaaaagc ctttagaagc
     1021 aagtcgaagc tcattcagca tcagcgtact cacactggag agagaccata ttcatgcaga
     1081 gaatgtggca aagcctttgc ccacatgtca gtcctcatta aacatgagaa aactcacata
     1141 agagagacag ccataaattc actgacggtg gagaaacctt cctcaaggag tcatacctcc
     1201 ttatacatga gcgaactcat acaagagcaa aagactgtga acacagtacc tatagaaatg
     1261 ccttcctcag gaaccccgcc attgttaaac aagagtgagc gcctagtggg cagaaatgta
     1321 gtgattgtgg aacaaccttt tccaagaaat caagcctttg tagttaatca ggaatttgaa
     1381 cagagaataa gcctcacaaa tgaagtgaat gtggccccat cagtaataaa ttatatcttg
     1441 tatcttacag atattgtatc agaatagtaa tgaactagtc cgtaacttga aagtatttcg
     1501 atttcttggc tttatatatc ttgtggaaag gacgattgtg taggaagact tcttggcga
//
