LOCUS       sequence-282621-        2438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1432
FEATURES             Location/Qualifiers
     source          1..2438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..2346
                     /codon_start=1
                     /gene="Homo sapiens NR3C1"
                     /product="human glucocorticoid receptor, isoform alpha"
                     /label=glucocorticoid receptor
                     /note="acts as a transcription factor that binds to 
                     glucocorticoid response elements, and as a modulator of 
                     other transcription factors"
                     /translation="MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASS
                     PSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGF
                     PQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDV
                     SSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDEN
                     CLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKE
                     DFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTAS
                     LSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMR
                     PDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGRQHNYLC
                     AGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPG
                     NKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAV
                     KWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRM
                     TLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTY
                     IKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEM
                     LAEIITNQIPKYSNGNIKKLLFHQK"
ORIGIN
        1 accgccacca tggactccaa agaatcatta actcctggta gagaagaaaa ccccagcagt
       61 gtgcttgctc aggagagggg agatgtgatg gacttctata aaaccctaag aggaggagct
      121 actgtgaagg tttctgcgtc ttcaccctca ctggctgtcg cttctcaatc agactccaag
      181 cagcgaagac ttttggttga ttttccaaaa ggctcagtaa gcaatgcgca gcagccagat
      241 ctgtccaaag cagtttcact ctcaatggga ctgtatatgg gagagacaga aacaaaagtg
      301 atgggaaatg acctgggatt cccacagcag ggccaaatca gcctttcctc gggggaaaca
      361 gacttaaagc ttttggaaga aagcattgca aacctcaata ggtcgaccag tgttccagag
      421 aaccccaaga gttcagcatc cactgctgtg tctgctgccc ccacagagaa ggagtttcca
      481 aaaactcact ctgatgtatc ttcagaacag caacatttga agggccagac tggcaccaac
      541 ggtggcaatg tgaaattgta taccacagac caaagcacct ttgacatttt gcaggatttg
      601 gagttttctt ctgggtcccc aggtaaagag acgaatgaga gtccttggag atcagacctg
      661 ttgatagatg aaaactgttt gctttctcct ctggcgggag aagacgattc attccttttg
      721 gaaggaaact cgaatgagga ctgcaagcct ctcattttac cggacactaa acccaaaatt
      781 aaggataatg gagatctggt tttgtcaagc cccagtaatg taacactgcc ccaagtgaaa
      841 acagaaaaag aagatttcat cgaactctgc acccctgggg taattaagca agagaaactg
      901 ggcacagttt actgtcaggc aagctttcct ggagcaaata taattggtaa taaaatgtct
      961 gccatttctg ttcatggtgt gagtacctct ggaggacaga tgtaccacta tgacatgaat
     1021 acagcatccc tttctcaaca gcaggatcag aagcctattt ttaatgtcat tccaccaatt
     1081 cccgttggtt ccgaaaattg gaataggtgc caaggatctg gagatgacaa cttgacttct
     1141 ctggggactc tgaacttccc tggtcgaaca gttttttcta atggctattc aagccccagc
     1201 atgagaccag atgtaagctc tcctccatcc agctcctcaa cagcaacaac aggaccacct
     1261 cccaaactct gcctggtgtg ctctgatgaa gcttcaggat gtcattatgg agtcttaact
     1321 tgtggaagct gtaaagtttt cttcaaaaga gcagtggaag gtagacagca caattaccta
     1381 tgtgctggaa ggaatgattg catcatcgat aaaattcgaa gaaaaaactg cccagcatgc
     1441 cgctatcgaa aatgtcttca ggctggaatg aacctggaag ctcgaaaaac aaagaaaaaa
     1501 ataaaaggaa ttcagcaggc cactacagga gtctcacaag aaacctctga aaatcctggt
     1561 aacaaaacaa tagttcctgc aacgttacca caactcaccc ctaccctggt gtcactgttg
     1621 gaggttattg aacctgaagt gttatatgca ggatatgata gctctgttcc agactcaact
     1681 tggaggatca tgactacgct caacatgtta ggagggcggc aagtgattgc agcagtgaaa
     1741 tgggcaaagg caataccagg tttcaggaac ttacacctgg atgaccaaat gaccctactg
     1801 cagtactcct ggatgtttct tatggcattt gctctggggt ggagatcata tagacaatca
     1861 agtgcaaacc tgctgtgttt tgctcctgat ctgattatta atgagcagag aatgactcta
     1921 ccctgcatgt acgaccaatg taaacacatg ctgtatgttt cctctgagtt acacaggctt
     1981 caggtatctt atgaagagta tctctgtatg aaaaccttac tgcttctctc ttcagttcct
     2041 aaggacggtc tgaagagcca agagctattt gatgaaatta gaatgaccta catcaaagag
     2101 ctaggaaaag ccattgtcaa gagggaagga aactccagcc agaactggca gcggttttat
     2161 caactgacaa aactcttgga ttctatgcat gaagtggttg aaaatctcct taactattgc
     2221 ttccaaacat ttttggataa gaccatgagt attgaattcc ccgagatgtt agctgaaatc
     2281 atcaccaatc agataccaaa atattcaaat ggaaatatca aaaaacttct gtttcatcaa
     2341 aagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2401 tgtggaaagg acgagccctt tgttggaagt tagaattt
//
