LOCUS       sequence-28282-e         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, 
            Katovich MJ, Semple-Rowland SL, Raizada MK
  TITLE     Efficient large-scale production and concentration of HIV-1-based 
            lentiviral vectors for use in vivo.
  JOURNAL   Physiol Genomics. 2003 Feb 6. 12(3):221-8.
  PUBMED    12488511
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Physiol 
            Genomics. 2003 Feb 6. 12(3):221-8."
COMMENT     Sequence Label: pNHP
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     376..394
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(432..454)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     554..573
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtcctccttt ggaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
       61 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
      361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
      421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      541 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gagcc
//
