LOCUS       sequence-282834-        1646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2092
FEATURES             Location/Qualifiers
     source          1..1646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttggc caaccatgga cttattctac tgcaacagtt aaacgctcag
       61 cgagagtttg gtttcctgtg tgactgcacg gttgcaatcg gcgatgtata cttcaaggca
      121 cacaaatcag ttcttgcttc attctccaat tactttaaga tgttgtttgt ccatcagacc
      181 agtgagtgtg tccgcttgaa accaactgac atacagcccg acatcttcag ctatctctta
      241 cacttgatgt acactgggaa gatggcgcct cagctcatcg acccggttcg attagaacag
      301 ggcatcaagt ttctgcacgc ctacccgctc attcaggaag ccagcctcgc cagccaggga
      361 gccttttctc accctgacca agttttccca ctggcttctt cattgtatgg cattcagatt
      421 gcagatcatc agttgagaca agccaccaag attgcttcag cacctgaaaa actcgggcga
      481 gatccacggc cacagacctc caggataagc caggagcagg tccctgaggc ctcacagctc
      541 tcccagctga cttcaaatct ggcccaggtg aatcggacaa atatgactcc ctcagacccc
      601 ctgcagacct cgctgtctcc agaacttgtt tccactcctg ttcctccccc tcctcccggg
      661 gaggagacca atctggaagc atcttcctcc gatgagcagc ctgcgtccct cacaatagcc
      721 cacgtcaagc caagcatcat gaagaggaat gggagctttc caaagtacta tgcctgccac
      781 ctgtgtggac ggcgcttcac tctccggagc agcttacgtg aacacctcca gatccacaca
      841 ggagtacctt tcacatctag ccaacaggga gaaagtcgcg tccccctgac tctctgtagc
      901 aatgcagctg acctcgggaa agatgccatg gaagtgcctg aagccgggat gataagtgac
      961 agtgagctgc agcacatctc tgattctccc atcatcgatg ggcagcagca gtcggaaacc
     1021 ccacccccct cagacattgc tgacattgac aacctggagc aggccgacca ggagagggag
     1081 gtgaagaggc ggaagtacga gtgcacaata tgtggacgca aatttatcca gaaaagccac
     1141 tggagggaac acatgtacat acacaccggg aagcctttca agtgcagcac ttgtgacaaa
     1201 agcttttgca gggccaacca ggctgcccgc cacgtgtgcc tcaaccagag catcgacact
     1261 tacaccatgg tggacaaaca gactctggaa ctctgcacat ttgaggaagg gagtcagatg
     1321 gacaacatgc tggtgcaaac aaacaaaccc tacaaatgca acttgtgtga caaaacattc
     1381 tccactccca atgaggttgt taaacattca tgccaaaacc agaactcgga tgtttttgcc
     1441 ctagacgaag ggcgatccat tctcctgggc agtggggact cggaagtaac ggagcctgac
     1501 cacccagtgt tagcttccat caaaaaggaa caagaaaccg tcttactaga ctagtaatga
     1561 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1621 gaagtctgag cgaataggaa caagac
//
