LOCUS       sequence-282873-        1703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2252
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatagata tcaaggaaat caagacagag ttttgaggca ggtcacagtc
       61 atcagtcgtg aaacattgac tgatgagatg ggttccaagt acagtgcatt tgggaaaatg
      121 ttcaatcggt gcacagacct tgctccttta agtcaaaaat tccataagtt tgattcatgt
      181 gaaaatagct tgaagtctaa ttcagactta ctaaattata acaggagcta tgcaagaaag
      241 aaccccacta agagatttag atgtgggaga ccacctaagt ataatgcttc ctgttctgtg
      301 cctgagaagg aaggcttcat tcatactgga atggagccct atggagatag tcaatgtgaa
      361 aaagttctca gtcataagca agcccatgtt cagtataaga aatttcaagc cagagagaaa
      421 cccaatgttt gtagtatgtg tgggaaagcc tttatcaaga agtcacagct cattatacat
      481 caaagaattc atactggaga gaaaccatat gtatgtggag attgtaggaa agccttcagt
      541 gagaaatcac acctcattgt gcatcagagg attcatactg gggagaaacc ctatgaatgt
      601 actaagtatg gaagagcatt ctcccggaag tcacctttca ctgttcatca gagagtccat
      661 actggagaga aaccctatga gtgttttgag tgtccaaaag ctttctccca gaagtcacat
      721 ctaattatac atcagagagt tcataccaga gagaagccct ttgaatgcag tgaatgcagg
      781 aaagccttct gtgagatgtc tcaccttttt atacaccaga taactcatac tgggaagaag
      841 ccctatgaat gtactgaatg tgggaagacc ttccctcgga aaacacagct cattatacat
      901 cagagaacgc atactggaga gaagccctat aagtgtggtg aatgtgggaa aactttctgc
      961 caacagtccc acctcatagg acatcaaaga attcatacag gagaaaaacc ttatgtgtgt
     1021 actgactgtg ggaaggcctt ttcccagaag tcacacctca ctggccatca aagacttcat
     1081 actggagaga aaccttatat gtgtactgaa tgtggaaaat ccttctctca gaaatcacct
     1141 cttatcatac accagagaat tcatacaggg gagaaacctt atcagtgtgg tgaatgtggc
     1201 aaaaccttct cccagaaatc actcctcatt attcatctga gagttcacac aggggagaaa
     1261 ccttatgagt gtactgagtg tgggagggcc ttttccctga agtcacatct cattctacat
     1321 cagagaggtc atactggaga gaaaccctat gaatgtagtg aatgtggaaa ggccttctgt
     1381 ggaaagtctc cactcattat acatcagaaa actcatccta gggagaaaac ccctgaatgt
     1441 gctgagtctg gaatgacttt tttctggaaa tcacagatga ttacatatca gagaagacac
     1501 actggggaga aaccctccag atgcagtgac tgtgggaagg cattctgcca gcatgtatac
     1561 tttactgggc atcagaatcc atataggaaa gacaccttgt atatatgcta gtaatgaact
     1621 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1681 gtacacacgg tcattacgtt tgg
//
