LOCUS       sequence-282894-        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2371
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggtcta ttgacattca gggacgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctttatacag ggacgtgatg
      121 ctggagaatt ataggaacct cgtctccctg gatatctctt ccaaatgcat gttgaagacg
      181 ttgtcgtcaa cagggcaagg caatacagaa gtgatccaca cagggacatt gcacagacaa
      241 gcaagtcatc acattggaga attttgtttc catgaaattg agaaagacat tcatggcttc
      301 gagtttcagt ggaaagaaga tgaaacaaat ggccatgcag cacccatgac agaaatcaaa
      361 gagttggctg gtagtacagg ccaacatgat caaaggcatg ctggaaacaa gcgtattaaa
      421 gatcagcttg gatcaagctt tcatttgcat ctgcctgaac cgcacatatt tcagtctgaa
      481 gggaaaattg gtaatcaagt tgagaagtct atcaacaatg cttcctcagt ttcaacatcc
      541 caaagaattt gttgtaggcc caaaacccat atttctaata agtatggaaa taattccctc
      601 cattcttcat tactcacaca aaaatgggaa gtacacatga gagaaaaatc ttttgaatgt
      661 atacagagct tcaaatcctt taattgcagc tcactcttaa aaaaacatca gataattcac
      721 ttagaagaga aacaatgtaa atgtgatgta tgtggcaagg tctttaatca gaagcgatac
      781 cttgcctgcc atcgtagatg tcacactggt gagaaacctt acaagtgtaa tgagtgtggc
      841 aagacctttg gtcataattc atccctcttc attcacaaag cgcttcatac tggagagaaa
      901 ccttatgaat gtgaagaatg tgacaaagtt ttcagtcgca aatcacacct tgaaagacat
      961 aagaggattc atactggaga gaaaccatac aaatgtaagg tttgtgatga ggctttcgca
     1021 tataattcat atctggcaaa acatactata cttcacactg gagagaaacc ttacacatgt
     1081 aatgaatgtg gcaaagtttt taatcgacta tcaacccttg cacgccatca tagacttcat
     1141 actggagaga aaccttacaa atgtgaagag tgtgacaaag ttttcagtcg caaatcacac
     1201 cttgaaagac ataggaggat tcatagtgga gagaaacctt acaaatgtga agaatgttgc
     1261 aaagttttca gtcgcaaatc aaacctggaa agacacagga ggattcatac tggagagaaa
     1321 ccatacaaat gtaaggtttg tgacaaggct ttccagaggg attcacacct ggcacaacat
     1381 cagagagttc atactggaga gaaaccttac aagtgtaatg agtgtggcaa gaccttcggt
     1441 cagacatcat cgcttataat ccatcgtagg cttcatactg gagagaaacc ttacaagtgt
     1501 aatgagtgtg gcaagacctt cagtcagatg tcatcccttg tataccatca taggcttcat
     1561 agtggagaga agccttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1621 ctttatatat cttgtggaaa ggacgattgt cgtgaaagta gactactttg
//
