LOCUS       sequence-282912-        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2625
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcctc gccgggctcg ggcagcgcca acccgcggaa gttcagtgag
       61 aagatcgcgc tgcacacgca gagacaggcc gaggagacgc gggccttcga gcagctcatg
      121 accgacctca ccctgtcgcg ggttcaattt cagaagcttc agcaactgcg ccttacacag
      181 taccatggag gatccttacc aaatgtgagc cagctgcgga gcagtgcgtc agagtttcag
      241 ccgtcatttc accaagctga taatgttcgg ggaacccgcc atcacgggct ggtggagagg
      301 ccatccagga accgcttcca ccccctccac cgaaggtctg gggacaagcc agggcgacaa
      361 tttgatggta gtgcttttgg agccaattat tcctcacagc ctctggatga gagttggcca
      421 aggcagcagc ctccttggaa agacgaaaag catcctgggt tcaggctgac atctgcactt
      481 aacaggacca attctgattc tgctcttcac acgagtgctc tgagtaccaa gccccaggac
      541 ccctatggag gagggggcca gtcggcctgg cctgccccat acatgggttt ctgtgatggt
      601 gagaataatg gacatgggga agtagcatct ttccctggcc cattgaaaga agagaatctg
      661 ttaaatgttc cgaagccact gccaaaacaa ctgtgggaga ccaaggagat tcagtccctg
      721 tcaggacgcc ctcgatcctg tgatgttgga ggtggcaatg cttttccaca taatggtcaa
      781 aacctaggcc tctcaccctt cttggggacc ttgaacactg gagggtcatt gccagatcta
      841 accaacctcc actactcgac acccctgcca gcctccctgg acaccaccga ccaccacttt
      901 ggcagtatga gtgtggggaa tagtgtgaac aacatcccag ctgctatgac ccacctgggt
      961 ataagaagct cctctggtct ccagagttct cggagtaacc cctccatcca agccacgctc
     1021 aataagactg tgctttcctc ttccttaaat aaccacccac agacatctgt tcccaacgca
     1081 tctgctcttc acccttcgct ccgtctgttt tcccttagca acccatctct ttccaccaca
     1141 aacctgagcg gcccgtctcg gcgtcggcag cctcccgtca gccctctcac gctttctcct
     1201 ggccctgaag cacatcaagg tttcagcaga cagctgtctt caaccagccc actggcccca
     1261 tatcctacct cccagatggt gtcctcagac cgaagccaac tttcctttct gcccacagaa
     1321 gctcaagccc aggtgtcgcc gccaccccct taccctgcac cccaggagct cacccagccc
     1381 ctcctgcagc agccccgcgc ccctgaggcc cctgcccagc agccccaggc agcctcctca
     1441 ctgccacagt cagactttca gcttctcccg gcccagggct catctttgac caacttcttc
     1501 ccagatgtgg gttttgacca gcagtccatg aggccaggcc ctgcctttcc tcaacaggtg
     1561 cctctggtgc aacaaggttc ccgagaactg caggactctt ttcatttgag accaagcccg
     1621 tattccaact gcgggagtct cccgaacacc atcctgccag aagactccag caccagcctg
     1681 ttcaaagacc tcaacagtgc gctggcaggc ctgcctgagg tcagcctgaa cgtggacact
     1741 ccatttccac tggaagagga gctgcagatt gaacccctga gcctggacgg actcaacatg
     1801 ttaagtgact ccagcatggg cctgctggac ccctctgttg aagagacgtt tcgagctgac
     1861 agactgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1921 tcttgtggaa aggacgaaag tttgttcagg catattcggc g
//
