LOCUS       sequence-282972-        5174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2771
FEATURES             Location/Qualifiers
     source          1..5174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..312
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggcgggcgt ggggccgggg ggctacgcgg cggagttcgt gccaccgcca
       61 gagtgccccg tctttgagcc gagttgggag gagttcacag atccgctcag ctttatcggc
      121 cgcatccggc ctttggcgga gaaaaccggc atctgcaaaa ttcggccgcc caaggactgg
      181 cagcctccat ttgcctgtga agtaaaaagc tttcgtttca ctccaagagt ccagcgcctg
      241 aatgaacttg aggcaatgac cagagtgaga ttggatttct tggatcaact agcaaaattt
      301 tgggaacttc aaggatctac tctgaagatc cctgtggtag agagaaaaat cctggatctg
      361 tatgctttga gcaagattgt tgccagcaaa ggaggttttg aaatggtcac caaagagaag
      421 aaatggtcta aagtgggtag tcgcttggga tatctgccag gaaaaggaac tgggtctctt
      481 ttgaagtcac attatgaaag aattctctac ccatatgagc ttttccagtc tggtgtgagc
      541 cttatgggtg tgcagatgcc taatttagat cttaaagaaa aagtggagcc tgaggttctc
      601 agcactgata cccaaacttc cccagagcca ggcacaagga tgaacattct gccgaagaga
      661 acaagacgtg tgaagactca gtcagaatct ggagatgtga gtagaaacac ggaactgaag
      721 aaacttcaga tttttggggc tgggcccaag gttgtgggct tggcaatggg aacaaaagat
      781 aaagaagatg aggtcacccg aagacgaaaa gttaccaaca ggtcagacgc atttaacatg
      841 caaatgagac aacggaaagg cactctctct gttaactttg ttgatctcta tgtttgtatg
      901 ttttgtggtc ggggaaacaa tgaagataaa ttgcttttgt gtgatggatg tgatgacagc
      961 tatcatacat tttgtctaat tcctccacta cctgatgtgc ccaaaggaga ctggaggtgt
     1021 cctaaatgtg tcgccgagga atgtagcaaa cctcgagaag cctttggatt tgaacaagct
     1081 gtacgagagt atacacttca gagctttgga gagatggcag ataattttaa gtctgattat
     1141 tttaatatgc cagtccatat ggttcccaca gaactagtag aaaaggaatt ttggcggctg
     1201 gtaagcagca ttgaagaaga tgttattgtg gaatatggag cagatatctc ctcaaaagac
     1261 tttggaagtg gatttccggt gaaggatggg cggagaaaga ttctgccaga agaagaggaa
     1321 tatgcacttt ctggttggaa tttgaataac atgcctgtcc tggaacagtc tgttcttgca
     1381 catattaatg tggacatctc tggtatgaaa gtgccgtggc tctatgtggg aatgtgcttc
     1441 tcttcttttt gctggcacat tgaggatcac tggagttatt ccatcaacta cttgcactgg
     1501 ggggagccaa agacatggta tggtgtgcca tctcatgctg cagagcaact ggaggaggtg
     1561 atgagagagc tggcccccga gttatttgaa tcccagcctg atcttctgca tcagttagtt
     1621 accatcatga accccaacgt gctaatggag catggtgtgc ctgtgtacag gaccaatcag
     1681 tgtgctggcg agtttgttgt gacatttcct cgtgcctatc actctggatt taaccagggc
     1741 tacaactttg ctgaagctgt gaacttctgt actgctgact ggttgcccat tggacgtcaa
     1801 tgtgtaaatc attaccgacg cctaaggcgc cactgtgtct tttcacacga ggaactaatt
     1861 ttcaagatgg cagcagatcc agaatgctta gatgtggggc tggctgccat ggtctgcaaa
     1921 gaattgactc tcatgactga agaagaaaca cgattaagag agtctgttgt acagatgggt
     1981 gtcctgatgt cagaagaaga agtgtttgaa cttgttcctg atgatgagcg gcagtgttca
     2041 gcatgcagaa ccacatgttt tctctctgct ctcacatgtt cctgtaatcc tgagcggctt
     2101 gtatgtctct accatccaac tgatctgtgc ccctgcccca tgcagaagaa atgtcttaga
     2161 tatcgctacc cattagaaga cctcccttct ctgctatatg gtgtaaaagt cagggcacag
     2221 tcctatgaca cttgggtcag tcgtgttaca gaagcattgt ctgctaactt caaccacaaa
     2281 aaagatttga ttgaattgcg agtaatgctg gaagatgctg aggataggaa atacccagag
     2341 aatgatctct ttcgaaaact cagggatgct gtaaaagaag ctgagacctg tgcttctgtg
     2401 gctcagctgc ttctgagcaa aaagcagaaa cacagacaga gcccagatag tgggaggact
     2461 cggaccaaac tgacagtgga agaattgaag gcctttgtcc aacaactttt tagtcttccg
     2521 tgtgtcatca gccaagctcg gcaagtaaag aatctgctag atgatgtgga agagtttcat
     2581 gaacgtgctc aggaggccat gatggatgaa accccagatt cttccaaact ccagatgttg
     2641 atagatatgg gctctagtct ctatgtggaa ctccctgaat taccacgact gaagcaagag
     2701 ctacaacagg ctcggtggtt ggacgaagta agactgacct tatcagatcc gcaacaagtc
     2761 actttggatg tcatgaagaa gctgatagac tctggggtag ggttggcacc ccaccatgct
     2821 gtggagaaag caatggctga actacaggag ctccttacag tctctgaacg atgggaagaa
     2881 aaggctaagg tctgcctaca ggcaagaccg aggcacagtg tggcaagttt agaaagcatt
     2941 gtgaatgaag ccaagaacat tccagccttt ctacccaatg tgttgtcctt gaaagaagcc
     3001 ttacaaaagg ctcgagaatg gaccgctaaa gtggaagcta ttcagagtgg cagcaattac
     3061 gcttatttgg agcagcttga gagcttgtct gcgaaaggac gccctattcc tgtgcgtctt
     3121 gaagcactgc cgcaagtgga atcacaggta gcagcagcac gggcatggag agaacggact
     3181 gggcggacgt ttcttaagaa gaattctagc catacattgt tacaggtgct gagcccccgg
     3241 accgacattg gtgtatatgg gagtggcaaa aataggagga aaaaagtaaa agaactaata
     3301 gaaaaagaaa aagaaaagga tctggacctg gagcctctga gtgatctgga ggaaggattg
     3361 gaggaaacca gagatacagc catggtggtg gcagttttca aagaacggga gcaaaaagag
     3421 attgaagcca tgcattctct cagagcagcc aacctagcca agatgacaat ggtggaccgc
     3481 atagaagaag taaaattttg catttgccgc aagacagcca gtgggtttat gctacagtgt
     3541 gagctctgca aagactggtt ccataacagc tgtgttcctc ttcctaaatc aagttcccaa
     3601 aaaaaaggat ccagctggca agctaaagaa gtaaaattcc tttgccctct ttgtatgcgg
     3661 tctcgaaggc ccaggctaga gactattctg tcactcctgg tatcccttca gaagttgccc
     3721 gtacggttgc ctgaaggaga ggccctgcag tgtttgacag aacgtgctat gagttggcaa
     3781 gatagagcgc ggcaggctct agccacagat gaactatcct ctgccctggc caaactatct
     3841 gtgttgagcc agcgtatggt ggaacaggcg gctcgagaaa aaactgaaaa gatcatcagt
     3901 gcagaactcc aaaaagcagc tgccaatcca gacttacagg gacacttacc tagtttccag
     3961 cagtctgctt ttaaccgggt ggtgagcagt gtgtcatctt ctcctcgaca aacaatggac
     4021 tatgatgatg aagaaacaga ctctgatgaa gacattcgag agacatatgg ctacgacatg
     4081 aaggacacag ccagtgtgaa gtcctctagt agtcttgaac ccaatctttt ttgtgatgaa
     4141 gagattccca tcaaatccga ggaggtggtg acccacatgt ggacagcacc ttcattttgt
     4201 gcagagcatg cttattcttc tgcttctaag agttgttctc aaggttctag caccccaagg
     4261 aaacaacctc ggaagagccc tttggtgccc cgaagtttgg aacctccagt gctggagttg
     4321 tcacctggag ctaaggcaca actggaagaa cttatgatgg ttggagatct cctggaagta
     4381 tctctggacg agactcaaca catatggcgg attttgcagg ccacacaccc accctctgaa
     4441 gacagattct tgcatatcat ggaggatgac agcatggaag agaaaccact aaaagtgaaa
     4501 ggaaaggact cttcagagaa gaaacggaaa cggaagctag aaaaggtaga gcaacttttt
     4561 ggagaaggaa aacagaagtc caaggagtta aagaaaatgg acaaacctag aaagaagaaa
     4621 ttaaaattag gtgcagacaa atcaaaggag ctgaataaac tggccaagaa actagcaaaa
     4681 gaagaagaga gaaagaaaaa gaaggagaag gctgctgcag ccaaagttga acttgtgaaa
     4741 gagagcactg aaaagaaaag agagaaaaag gtgctggaca tcccctcaaa gtatgactgg
     4801 tcaggagcag aggagtctga tgatgagaat gctgtgtgcg cagcacagaa ctgccaaagg
     4861 ccctgcaagg acaaggtaga ctgggtacaa tgtgatggtg gctgtgatga gtggtttcat
     4921 caagtttgtg tgggtgtatc tccagaaatg gctgaaaatg aagattacat ctgtataaac
     4981 tgtgcaaaga agcaggggcc agttagccca ggtccagcac cacctccttc cttcataatg
     5041 agctacaaac taccaatgga ggatcttaaa gagaccagtt agtaatgaac tagtccgtaa
     5101 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga agccggcacg
     5161 acactcctgg aggc
//
