LOCUS       sequence-28308-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: phGluc
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..624
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
ORIGIN
        1 tngttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac ctcggatcca
       61 gccaccatgg gagtcaaagt tctgtttgcc ctgatctgca tcgctgtggc cgaggccaag
      121 cccaccgaga acaacgaaga cttcaacatc gtggccgtgg ccagcaactt cgcgaccacg
      181 gatctcgatg ctgaccgcgg gaagttgccc ggcaagaagc tgccgctgga ggtgctcaaa
      241 gagatggaag ccaatgcccg gaaagctggc tgcaccaggg gctgtctgat ctgcctgtcc
      301 cacatcaagt gcacgcccaa gatgaagaag ttcatcccag gacgctgcca cacctacgaa
      361 ggcgacaaag agtccgcaca gggcggcata ggcgaggcga tcgtcgacat tcctgagatt
      421 cctgggttca aggacttgga gcccatggag cagttcatcg cacaggtcga tctgtgtgtg
      481 gactgcacaa ctggctgcct caaagggctt gccaacgtgc agtgttctga cctgctcaag
      541 aagtggctgc cgcaacgctg tgcgaccttt gccagcaaga tccagggcca ggtggacaag
      601 atcaaggggg ccggtggtga ctaagcggcc gctcgagcat gcatctagca ttatccctaa
      661 tacctgccac cccactctta atcagtggtg gaagaacggt ctcagactgt ttgttcattg
      721 gcatttaagt ttagtagtaa agactggtta tgataacatg catcgtaacc ct
//
