LOCUS       sequence-28309-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: phGluc
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..614)
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atagagtggg gtggcaggta ttagggataa tgctagatgc atgctcgagc ggccgcttag
       61 tcaccaccgg cccccttgat cttgtccacc tggccctgga tcttgctggc aaaggtcgca
      121 cagcgttgcg gcagccactt cttgagcagg tcagaacact gcacgttggc aagccctttg
      181 aggcagccag ttgtgcagtc cacacacaga tcgacctgtg cgatgaactg ctccatgggc
      241 tccaagtcct tgaacccagg aatctcagga atgtcgacga tcgcctcgcc tatgccgccc
      301 tgtgcggact ctttgtcgcc ttcgtaggtg tggcagcgtc ctgggatgaa cttcttcatc
      361 ttgggcgtgc acttgatgtg ggacaggcag atcagacagc ccctggtgca gccagctttc
      421 cgggcattgg cttccatctc tttgagcacc tccagcggca gcttcttgcc gggcaacttc
      481 ccgcggtcag catcgagatc cgtggtcgcg aagttgctgg ccacggccac gatgttgaag
      541 tcttcgttgt tctcggtggg cttggcctcg gccacagcga tgcagatcag ggcaaacaga
      601 actttgactc ccatggtggc tggatccgag gtaggcgccg gtcacagctt ggatctgtaa
      661 cggcgcagaa cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc
      721 gtggagagcc ggctgagtca gancanccca agg
//
