LOCUS       sequence-283161-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2926
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(275..286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgggttcccc cgccgccccg gagggagcgc tgggctacgt ccgcgagttc
       61 actcgccact cctccgacgt gctgggcaac ctcaacgagc tgcgcctgcg cgggatcctc
      121 actgacgtca cgctgctggt tggcgggcaa cccctcagag cacacaaggc agttctcatc
      181 gcctgcagtg gcttcttcta ttcaattttc cggggccgtg cgggagtcgg ggtggacgtg
      241 ctctctctgc ccgggggtcc cgaagcgaga ggcttcgccc ctctattgga cttcatgtac
      301 acttcgcgcc tgcgcctctc tccagccact gcaccagcag tcctagcggc cgccacctat
      361 ttgcagatgg agcacgtggt ccaggcatgc caccgcttca tccaggccag ctatgaacct
      421 ctgggcatct ccctgcgccc cctggaagca gaacccccaa cacccccaac ggcccctcca
      481 ccaggtagtc ccaggcgctc cgaaggacac ccagacccac ctactgaatc tcgaagctgc
      541 agtcaaggcc cccccagtcc agccagccct gaccccaagg cctgcaactg gaaaaagtac
      601 aagtacatcg tgctaaactc tcaggcctcc caagcaggga gcctggtcgg ggagagaagt
      661 tctggtcaac cttgccccca agccaggctc cccagtggag acgaggcctc cagcagcagc
      721 agcagcagca gcagcagcag tgaagaagga cccattcctg gtccccagag caggctctct
      781 ccaactgctg ccactgtgca gttcaaatgt ggggctccag ccagtacccc ctacctcctc
      841 acatcccagg ctcaagacac ctctggatca ccctctgaac gggctcgtcc actaccggga
      901 agtgaatttt tcagctgcca gaactgtgag gctgtggcag ggtgctcatc ggggctggac
      961 tccttggttc ctggggacga agacaaaccc tataagtgtc agctgtgccg gtcttcgttc
     1021 cgctacaagg gcaaccttgc cagtcatcgt acagtgcaca caggggaaaa gccttaccac
     1081 tgctcaatct gcggagcccg ttttaaccgg ccagcaaacc tgaaaacgca cagccgcatc
     1141 cattcgggag agaagccgta taagtgtgag acgtgcggct cgcgctttgt acaggtggca
     1201 catctgcggg cgcacgtgct gatccacacc ggggagaagc cctacccttg ccctacctgc
     1261 ggaacccgct tccgccacct gcagaccctc aagagccacg ttcgcatcca caccggagag
     1321 aagccttacc actgcgaccc ctgtggcctg catttccggc acaagagtca actgcggctg
     1381 catctgcgcc agaaacacgg agctgctacc aacaccaaag tgcactacca cattctcggg
     1441 gggccctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1501 tcttgtggaa aggacgagac gatccgcaag tgacactacc g
//
