LOCUS       sequence-283221-        3314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0377
FEATURES             Location/Qualifiers
     source          1..3314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcagga cactgccgca gtggcagcca ccgtggcagc cgcggatgcg
       61 accgccacta tcgtggtcat agaggacgag cagcccgggc cgtccacctc tcaggaggag
      121 ggagcggccg ccgcggccac cgaagccacc gcggccacgg agaagggcga gaagaagaag
      181 gagaaaaacg tttcttcatt tcaactcaaa cttgctgcta aagcgcctaa atctgaaaag
      241 gaaatggacc cagaatatga agagaaaatg aaagccgacc gagcaaagag atttgaattt
      301 ttactgaagc agacagaact ttttgcacat ttcattcagc cttcagcaca gaaatctcca
      361 acatctccac tgaacatgaa attgggacgt ccccgaataa agaaagatga aaagcagagc
      421 ttaatttctg ctggagacta ccgccatagg cgcacagagc aagaagaaga tgaagagcta
      481 ctgtctgaga gtcggaaaac atctaatgtg tgtattagat ttgaggtgtc accttcatat
      541 gtgaaagggg ggccactgag agattatcag attcgaggac tgaattggtt gatctcttta
      601 tatgaaaatg gagtcaatgg cattttggct gatgaaatgg gccttgggaa aactttacaa
      661 acaattgctt tgcttggtta cctgaaacac taccgaaata ttcctggacc tcacatggtt
      721 ttagttccaa agtctacttt acacaactgg atgaatgaat ttaaacgatg ggtcccatct
      781 ctccgtgtca tttgttttgt cggagacaag gatgccagag ctgcttttat tcgtgatgaa
      841 atgatgccag gagagtggga tgtttgcgtt acttcttatg agatggtaat taaagaaaaa
      901 tctgtattca aaaagtttca ctggcgatac ctggtcattg atgaagctca cagaataaag
      961 aatgaaaaat ctaagctttc agagattgtt cgtgagttca agtcgactaa ccgcttgctc
     1021 ctaactggaa cacctttgca gaataacctg catgaactgt gggccttact caacttttta
     1081 ttgcctgatg tctttaattc tgcagatgac tttgattctt ggtttgacac taaaaattgt
     1141 cttggtgatc aaaaactcgt ggaaagactt catgcagttt taaaaccatt tttgttacgc
     1201 cgtataaaaa ctgatgtaga gaagagtctg ccacctaaaa aggaaataaa gatttacttg
     1261 gggctgagta agatgcaacg agaatggtat acaaaaatcc tgatgaaaga tattgatgtt
     1321 ttaaactctt ctggcaagat ggacaagatg cgactcttaa acattctgat gcagcttcga
     1381 aagtgttgta atcatccata tctgtttgat ggtgctgaac ctggtccacc ttataccact
     1441 gatgagcata ttgtcagcaa cagtggtaaa atggtagttc tggataaact attggccaaa
     1501 ctcaaagaac agggttcaag ggttctcatt ttcagccaga tgactcgctt gctggatatt
     1561 ttggaagatt attgcatgtg gcgtggttat gagtattgtc gactggatgg acaaaccccg
     1621 catgaagaaa gagaggataa attcctagaa gtggaatttc tgggtcaaag ggaagcaata
     1681 gaggctttta atgctcctaa tagtagcaaa ttcatcttta tgctaagtac cagggctgga
     1741 ggtctcggaa ttaacctggc aagtgctgat gtggttatac tatatgattc agactggaac
     1801 ccacaggttg atctacaagc tatggatcga gcacatcgta ttggtcagaa gaaaccagta
     1861 cgtgtattcc gtctcatcac tgacaacact gttgaagaga ggattgtaga aagagctgag
     1921 ataaaactga gactcgattc aattgttata caacaaggaa gactcattga ccaacagtct
     1981 aacaagctgg caaaagagga aatgttacaa atgatacggc atggagccac ccatgttttt
     2041 gcttctaaag agagtgagtt gacagatgaa gacattacaa ctattctgga aagaggggaa
     2101 aagaagactg cagagatgaa tgaacgcctg caaaaaatgg gagagtcttc tctaagaaat
     2161 tttagaatgg acattgaaca aagtttatac aaatttgagg gagaagatta tagagaaaaa
     2221 cagaagcttg gcatggtgga atggattgaa cctcctaaac gagaacgcaa agcaaactac
     2281 gcagtggatg cctactttag agaggctttg cgtgtcagcg agccaaagat tccaaaggct
     2341 ccacggcctc caaaacagcc aaatgttcag gattttcaat ttttcccacc acgcttattt
     2401 gagctcctgg aaaaggaaat tctttattat cggaagacaa taggctataa ggttccaagg
     2461 aatcctgata tcccaaatcc agctctggct caaagagaag agcaaaaaaa gattgatgga
     2521 gctgaacctc ttacaccaga agagactgaa gaaaaggaaa aacttctcac acaaggtttc
     2581 acaaactgga ctaaacgaga ttttaaccag tttattaaag ctaatgagaa atatggaaga
     2641 gatgacattg ataacatagc tcgagaggta gagggcaaat cccctgagga ggtcatggag
     2701 tattcagctg tattttggga acgttgcaat gaattacagg acattgagaa aattatggct
     2761 caaattgaac gtggagaagc aagaattcaa cgaaggatca gtatcaagaa agccctggat
     2821 gccaaaattg caagatacaa ggctccattt catcagttgc gcattcagta tggaaccagc
     2881 aaaggaaaga actatactga ggaagaagat agattcttga tttgtatgtt acacaaaatg
     2941 ggctttgata gagaaaatgt atatgaagaa ttaagacagt gtgtacgaaa tgctccccag
     3001 tttagatttg actggtttat caagtctagg actgccatgg aattccagag acgctgtaac
     3061 actctgattt cattgattga gaaagaaaat atggaaattg aggaaagaga gagagcagaa
     3121 aagaagaaac gggcaactaa aactccaatg tcacagaaaa gaaaagcaga gtcagctact
     3181 gagagctctg gaaagaagga tgtcaagaag gtgaaatcct agtaatgaac tagtccgtaa
     3241 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtcgttgcgt
     3301 ttcccgaccc tcta
//
