LOCUS       sequence-283296-        6281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0658
FEATURES             Location/Qualifiers
     source          1..6281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcccc tctgagggac gaggaggagg aggaggagga gatggtggtg
       61 tcggaggagg aagaagagga ggaagaagag ggcgacgagg aggaggagga ggaggtggag
      121 gcggccgacg aggacgatga ggaggacgac gacgagggag tactcgggcg cgggccgggc
      181 cacgaccggg gccgcgaccg ccacagcccc cccggctgcc acctcttccc gccgccgccg
      241 ccgccgccgc caccgctgcc cccgccgccg ccgcccccgc cgccagataa ggatgacatt
      301 cggctgctgc cgtcagcatt gggtgtgaag aagagaaaac gaggacccaa gaagcagaag
      361 gagaacaagc caggaaaacc ccgaaaacgc aagaagcgtg acagtgagga ggaatttggt
      421 tctgagcgag atgagtaccg ggagaagtca gagagtgggg gcagtgaata tggaaccgga
      481 ccgggtcgga aacgaagaag gaagcaccga gaaaaaaagg agaagaagac aaagcggcgg
      541 aaaaaggggg agggagatgg ggggcaaaag caagtggaac agaagtcatc agcaactctg
      601 cttctgacct ggggcctgga ggatgtggag catgtgttct ctgaggagga ttaccacacg
      661 ctcaccaact acaaagcctt cagccagttc atgaggcccc taattgctaa gaagaatcct
      721 aagatcccaa tgtctaagat gatgaccatc cttggggcca aatggagaga gttcagtgcc
      781 aacaacccct tcaaggggtc agcagctgct gtggcggcgg cagcggcagc agcagcagca
      841 gctgtagctg agcaggtgtc agctgctgtc tcgtcggcca cccccatagc accctccgga
      901 ccccccgccc ttccaccacc ccctgctgct gatatccagc ccccacccat ccgaagagcc
      961 aaaaccaaag agggcaaagg tccaggccat aagaggcgga gtaagagccc ccgagtgcct
     1021 gatggacgca agaagcttcg gggaaagaaa atggcaccac tcaaaataaa actagggctt
     1081 ctgggtggca agaggaagaa aggaggctcg tatgtttttc agagcgacga aggtcctgaa
     1141 ccagaggctg aggaatcaga cctggacagt ggcagtgtcc acagtgcctc aggccggcct
     1201 gatggccctg tccgcaccaa gaaactaaag agaggccggc caggaaggaa gaagaagaag
     1261 gtcctgggct gtcctgcagt ggccggggag gaggaggttg atggctacga gacggatcac
     1321 caggattact gtgaggtgtg ccagcagggt ggggaaatta ttctgtgtga cacctgccct
     1381 cgtgcctacc acctcgtctg ccttgatcct gagcttgacc gggctccaga gggcaaatgg
     1441 agctgccctc actgtgagaa ggagggggtc cagtgggagg ccaaggagga agaagaagaa
     1501 tacgaagagg agggagagga agaaggggag aaggaggagg aggatgatca catggagtac
     1561 tgccgcgtat gcaaggacgg cggggagctc ctgtgctgtg acgcgtgcat ctcctcctac
     1621 cacattcatt gtctaaaccc tcccctgcct gacattccca atggtgaatg gctgtgtccc
     1681 cgatgcacat gccccgtgct gaagggtcga gtgcagaaga tcctacattg gcggtggggg
     1741 gagccacctg tagcagtgcc agcccctcaa caggcagatg gaaatccaga tgtcccaccc
     1801 ccccgtcctc ttcaaggcag atcagagcga gagttctttg tcaagtgggt aggactatcc
     1861 tactggcact gctcctgggc caaggagctt cagctggaaa tcttccattt ggttatgtat
     1921 cgaaactacc agcggaagaa tgacatggat gagcccccac ccctggacta tggctccggc
     1981 gaggatgatg ggaagagcga caagcgtaaa gtgaaagacc cgcactatgc tgagatggag
     2041 gagaagtact atcgttttgg catcaagcca gagtggatga ccgtccaccg catcatcaac
     2101 cacagtgtgg ataaaaaggg gaattaccac tatctagtaa aatggaggga cttaccatat
     2161 gaccagtcca cgtgggagga agatgaaatg aatatccctg aatacgaaga acataagcaa
     2221 agctactgga gacaccgaga actaattatg ggggaagacc ctgcccagcc ccgcaagtat
     2281 aagaagaaga agaaggagct acagggtgat gggcctccca gttctcccac taatgatcct
     2341 accgtgaaat atgagactca gccacggttt atcacagcca ctggaggcac cctgcacatg
     2401 tatcagttgg aagggctgaa ctggctacgc ttctcctggg cccagggcac tgacaccatt
     2461 ctagctgatg agatggggct aggcaagacc atacaaacca tcgtcttcct ctactcactc
     2521 tacaaggagg gccacacaaa aggtcccttc ctggtgagtg ccccactctc taccatcatt
     2581 aactgggagc gggagttcca gatgtgggca cccaaattct atgtggtgac atacacgggt
     2641 gacaaggaca gccgggccat cattcgtgag aatgaattct cctttgagga caatgccatc
     2701 aaagggggca agaaagcttt taagatgaag agggaggcac aggtgaagtt ccatgttctc
     2761 ctgacatcgt atgagctgat caccattgat caggcagcac ttggttccat ccgctgggcc
     2821 tgtcttgtgg tagatgaggc ccatcgactc aagaacaacc agtccaagtt tttcagggtt
     2881 ctcaatggtt acaagataga tcataagttg ctgctgacag gaaccccatt gcagaataat
     2941 ctggaggagc tcttccatct cctgaacttc ctcaccccag agagatttaa caacttggag
     3001 ggcttcctgg aggagtttgc tgacatatcc aaagaggacc agatcaagaa actgcatgat
     3061 ttgctggggc cacacatgct gcggagactc aaggcagatg tctttaagaa catgccagcc
     3121 aagacagagc tcatcgttcg ggtggagcta agccccatgc agaagaaata ctacaaatac
     3181 atcctgactc gaaattttga ggccttgaat tcacgaggtg gtgggaacca ggtgtcgctg
     3241 cttaatatca tgatggatct taagaagtgc tgcaaccatc catacctttt tcccgtggct
     3301 gctatggagt cccccaaact ccccagtggg gcttatgagg gtggggcact tattaagtcg
     3361 tctgggaagc tcatgctgct ccagaagatg ctgcgaaagc tgaaggagca aggacaccga
     3421 gtgctcatct tctcgcagat gaccaaaatg ttagacttgc ttgaggactt cttagactat
     3481 gaaggctaca agtatgagcg catcgatggt ggtatcacgg gtgccctgag gcaggaggcc
     3541 atcgatcggt ttaatgctcc tggggcccaa caattctgct tcctcctgtc cacccgagct
     3601 gggggcctgg gcatcaatct ggccactgct gacactgtca tcatctttga ttctgactgg
     3661 aacccccata atgacatcca ggcctttagc cgggctcatc ggattggcca ggccaacaaa
     3721 gtgatgattt accggtttgt gactcgcgcg tcagtggaag agcgaatcac acaagtggcc
     3781 aagagaaaga tgatgctgac acacctggtt gtgcggcctg ggctgggctc caaggcaggc
     3841 tccatgtcca agcaggagct tgacgacatt ctcaaatttg gcactgaaga gctattcaag
     3901 gatgaaaacg agggggagaa caaggaggag gacagcagtg tgattcatta tgacaatgag
     3961 gccatcgctc ggctgttgga ccggaaccag gatgcaactg aggacactga cgtgcagaac
     4021 atgaatgagt atctcagctc cttcaaggtg gcacagtacg tcgtgcggga agaagacaag
     4081 attgaggaaa ttgagcgaga gatcatcaag caggaggaga atgtggaccc tgactactgg
     4141 gagaagctgc tgaggcatca ctatgagcaa cagcaggaag acctagcccg gaatctaggc
     4201 aagggcaagc gggttcgcaa gcaagttaac tacaatgatg ctgctcagga agaccaagac
     4261 aaccagtcag agtactcggt gggttcagag gaggaggatg aagacttcga tgaacgtcct
     4321 gaagggcgta gacagtcaaa gaggcagctc cggaatgaga aagataagcc actgcctcca
     4381 ctgctggccc gagtcggggg caacattgag gtgctgggct tcaacacccg tcagcggaag
     4441 gctttcctca atgctgtgat gcgctggggg atgccaccac aggatgcctt caccacacag
     4501 tggctggtgc gggacctgag gggcaagact gagaaggagt ttaaggccta tgtgtctttg
     4561 ttcatgcgcc atctgtgtga gcctggggca gacggctctg aaacctttgc cgatggggtc
     4621 cctcgggagg gactgagtcg ccagcaggtg ttgacccgca ttggagtcat gtctctcgtc
     4681 aaaaagaagg tgcaggagtt tgagcacatc aatgggcgtt ggtcaatgcc ggaactgatg
     4741 cctgacccca gcgccgattc taagcgctcc tccagagcct cctctcctac caaaacgtct
     4801 cccaccactc ctgaggcttc tgctaccaac agtccctgca cctctaaacc tgctactcca
     4861 gctccaagtg agaaaggaga aggcataagg acacctcttg agaaggagga agctgaaaac
     4921 caggaggaaa agccagagaa gaacagcaga attggggaga agatggagac agaggctgat
     4981 gcccccagcc cagccccatc acttggggag cggctggagc caaggaagat tcctctagag
     5041 gatgaggtgc caggggtgcc tggagagatg gagcctgaac ctgggtaccg tggggacaga
     5101 gagaagtcag ccacagagtc gacgccagga gaaagggggg aggagaagcc gttggatgga
     5161 caggaacaca gggagaggcc ggagggggaa acaggggatt tgggcaagag agaagatgta
     5221 aaaggtgacc gggagcttcg accagggcct cgagatgagc cacggtccaa tgggcgacga
     5281 gaggaaaaga cagagaagcc ccggttcatg ttcaatatcg ccgatggtgg cttcacagag
     5341 cttcacacac tgtggcagaa tgaggaacgg gcagctattt cctcggggaa actcaatgag
     5401 atctggcaca gaagacatga ctattggctt ctggctggga ttgtcctcca tggctatgca
     5461 cggtggcagg acatccagaa tgatgctcaa tttgccatta tcaacgagcc atttaaaact
     5521 gaagccaata aggggaactt tctggagatg aaaaataagt tcctggcccg gaggttcaag
     5581 ctcctggagc aggcgctggt gattgaggag cagctgcggc gggcggccta cctgaacctg
     5641 tcgcaggagc cggcgcaccc cgccatggcc ctccacgccc gcttcgccga ggccgagtgc
     5701 ctggccgaga gccaccagca cctctccaag gagtcgctgg cggggaacaa gccggccaac
     5761 gccgtcctgc acaaggttct gaaccagctg gaggagttgc tgagcgacat gaaggcggac
     5821 gtgacccgcc tgccagccac gctgtcccga atacccccca tcgcagcccg ccttcagatg
     5881 tccgagcgca gcatcctcag ccggctggcc agcaagggca cggagcctca ccccacaccg
     5941 gcctacccgc cgggtcccta cgctacacct ccggggtacg gggcggcctt cagcgccgca
     6001 cccgtagggg ccctggccgc cgcaggcgcc aattacagcc agatgcctgc agggtccttc
     6061 atcacagccg ccaccaacgg ccctccagtg cttgtgaaga aggagaagga aatggtgggg
     6121 gcattggtgt cagacgggct ggatcggaag gagccccgag ccggggaggt gatctgtata
     6181 gacgactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     6241 tcttgtggaa aggacgatag actgtggctg ggaatcggtc c
//
