LOCUS       sequence-283434-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1150
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaactga tctagaggtt ataataaagg ataatagtct tgtgctgaca
       61 ccatcacaca tcaaagccta catgttgatg actcttcaag gattagaata tttacatcaa
      121 cattggatcc tacataggga tctgaaacca aacaacttgt tgctagatga aaatggagtt
      181 ctaaaactgg cagattttgg cctggccaaa tcttttggga gccccaatag agcttataca
      241 catcaggttg taaccaggtg gtatcgggcc cccgagttac tatttggagc taggatgtat
      301 ggtgtaggtg tggacatgtg ggctgttggc tgtatattag cagagttact tctaagggtt
      361 ccttttttgc caggagattc agaccttgat cagctaacaa gaatatttga aactttgggc
      421 acaccaactg aggaacagtg gccggacatg tgtagtcttc cagattatgt gacatttaag
      481 agtttccctg gaataccttt gcatcacatc ttcagtgcag caggagacga cttactagat
      541 ctcatacaag gcttattctt atttaatcca tgtgctcgaa ttacggccac acaggcactg
      601 aaaatgaagt atttcagtaa tcggccaggg ccaacacctg gatgtcagct gccaagacca
      661 aactgtccag tggaaacctt aaaggagcaa tcaaatccag ctttggcaat aaaaaggaaa
      721 agaacagagg ccttagaaca aggaggattg cccaagaaac taatttttta gtaatgaact
      781 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      841 atgtaccggg cgagtgttta tat
//
