LOCUS       sequence-283440-        2000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1176
FEATURES             Location/Qualifiers
     source          1..2000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc ggcgctgcgg gaccccgcgc agggctgcgt gacctttgag
       61 gacgtgacca tttacttctc ccaggaggag tgggtgctcc tcgatgaggc tcagagactc
      121 ctgtactgcg atgtgatgct ggaaaacttt gcacttatag cctcgctggg acttatatct
      181 ttcaggtccc acatcgtttc ccagttggag atggggaaag agccctgggt gcctgacagt
      241 gtggatatga catcagccat ggccagaggg gcttatggca ggcctggctc tgatttttgc
      301 catggaacag agggcaagga cttaccttct gagcataacg tttctgtaga aggagtggca
      361 caggacagga gtcccgaggc aactctgtgc ccccagaaga cctgcccctg tgacatatgt
      421 ggcctacgtt tgaaagacat tttgcatttg gctgaacacc agacaacaca tcccaggcag
      481 aaaccatttg tgtgtgaggc atatgtgaaa ggctctgagt tcagtgcaaa ccttccccgg
      541 aaacaggtgc agcagaacgt acacaaccct atcagaacgg aggagggcca ggcttcccct
      601 gtaaagacct gcagagacca cacatcagat cagctttcca cctgcaggga gggtgggaag
      661 gactttgtgg ccacagcagg gtttctgcag tgtgaggtca ctcccagcga tggggagccg
      721 cacgaggcca ccgaaggtgt ggtggatttt cacattgcac taaggcataa caagtgctgt
      781 gaatctgggg atgccttcaa taacaaatcc actcttgttc agcaccagag aatccacagc
      841 agagaaaggc cttatgaatg cagcaaatgt ggaatcttct tcacttacgc cgctgacctc
      901 actcaacacc agaaagttca caatagagga aaaccgtatg agtgctgtga atgtggaaaa
      961 ttcttcagcc agcactccag ccttgttaaa catcgcaggg ttcacaccgg tgaaagccct
     1021 cacgtgtgtg gtgactgtgg gaaattcttc agccgaagct ccaacctcat tcagcataag
     1081 agggttcaca ctggtgaaaa gccatatgag tgcagcgact gtgggaagtt cttcagccag
     1141 cgttccaacc tcattcatca taagagggtt catacgggca gaagtgccca tgagtgcagt
     1201 gaatgtggga aatctttcaa ctgcaactcc agcctaatta aacattggag agttcacact
     1261 ggagaaagac cttacaagtg taacgaatgt gggaaattct tcagccacat tgccagcctc
     1321 attcaacatc agatagttca cactggcgag cggcctcacg ggtgtggtga gtgtgggaaa
     1381 gccttcagcc gaagctctga cctcatgaaa catcagcgag ttcacactgg tgagcggcct
     1441 tatgaatgca atgaatgtgg gaagttattt agccagagct ccagcctcaa tagccatcgg
     1501 agacttcaca ctggtgaacg gccttaccag tgcagtgaat gtgggaaatt ctttaaccaa
     1561 agctccagcc tcaataacca ccggagactt cacaccggcg agcggcctta tgagtgcagc
     1621 gaatgtggga aaaccttcag gcagaggtcc aatctgaggc agcacctgaa agttcacaaa
     1681 ccagacaggc cttacgaatg cagcgaatgt gggaaagcct tcaaccaaag gcctaccctc
     1741 attcggcatc agaagattca catcagagaa aggagcatgg agaatgtgct ccttccctgt
     1801 tcacagcaca caccagagat aagctctgag aacagacctt atcagggcgc tgtcaactac
     1861 aagttgaaac ttgttcatcc aagtacccac cctggggagg ttccctagta atgaactagt
     1921 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagacg
     1981 atctaatcta accaagtcag
//
