LOCUS       sequence-28346-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-EIF3S3
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..26
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             39..65
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggactacaa ggatgacgat gacaagctcg atggaggata cccatacgat gttccagatt
       61 acgctgctcg aggttatcaa acaagtttgt acaaaaaagt tggcatggca tcccgcaagg
      121 aaggtaccgg ctctactgcc acctcttcca gctccaccgc cggcgcagca gggaaaggca
      181 aaggcaaagg cggctcggga gattcagccg tgaagcaagt gcagatagat ggccttgtgg
      241 tattaaagat aatcaaacat tatcaagaag aaggacaagg aactgaagtt gttcaaggag
      301 tgcttttggg tctggttgta gaagatcggc ttgaaattac caactgcttt cctttccctc
      361 agcacacaga ggatgatgct gactttgatg aagtccaata tcagatggaa atgatgcgga
      421 gccttcgcca tgtaaacatt gatcatcttc acgtgggctg gtatcagtcc acatactatg
      481 gctcattcgt tacccgggca ctcctggact ctcagtttag ttaccagcat gccattgaag
      541 aatctgtcgt tctcatttat gatcccataa aaactgccca aggatctctc tcactaaagg
      601 catacagact gactcctaaa ctgatggaag tttgtaaaga aaaggatttt tcccctgaag
      661 cattgaaaaa agcaaat
//
