LOCUS       sequence-283473-        1823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1290
FEATURES             Location/Qualifiers
     source          1..1823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctaatgc agaagtgagt gtcccagtgg gggatgtggt tgtggtacct
       61 actgaaggaa atgaagggga gaatcctgaa gacactaaaa cccaagtgat tttgcagtta
      121 cagcctgtgc aacaaggttt gtttatcgat ggacactttt acaacaggat ttatgaagct
      181 gggtcggaga acaacacggc agttgtagca gtagaaactc acacgataca caaaattgaa
      241 gaagggattg atacaggcac tatagaagca aatgaggata tggaaattgc ttaccccata
      301 acttgtgggg agagcaaagc catcctcctc tggaagaagt ttgtatgtcc aggaataaac
      361 gtgaagtgtg tcaagttcaa tgatcagttg atcagcccca agcactttgt tcatctggct
      421 ggcaagtcca ctctgaagga ctggaagaga gctattcgtc tgggtgggat catgctcagg
      481 aaaatgatgg actccggaca gattgatttt taccaacatg acaaagtttg ctccaatacc
      541 tgcagaagca ccaaatttga tcttctgatc agcagtgcaa gagctccagt gccaggacag
      601 cagacaagtg tggtgcagac acccacttcg gctgatggta gcatcacgca gattgccatc
      661 tcagaagaga gcatggaaga ggcagggctg gaatggaact cagctctcac cgctgctgtc
      721 accatggcca cggaggaggg tgtaaagaaa gactcagagg aaatttcaga ggacactttg
      781 atgttctgga aaggaatagc tgatgtaggg ctgatggaag aggttgtctg caatatacag
      841 aaggaaatag aggagctact caggggagtt cagcagcggc tcatccaggc tcccttccaa
      901 gtcacagatg ctgctgttct caacaatgta gcacacacat ttggcctaat ggacacagtc
      961 aagaaggttt tagacaacag aaggaaccaa gtagagcagg gagaagaaca gtttctctat
     1021 actctgacag acttggaacg ccagttggag gagcagaaga agcaaggcca ggatcacagg
     1081 ctgaaatctc agacagttca aaatgtggta ctgatgcctg tgagcactcc taagcctcca
     1141 aaaaggcccc ggctccagcg gccagcctcc accactgtct tgagcccttc tcctcctgtc
     1201 cagcagcctc agttcacagt catctcaccc atcaccatca ccccagtggg tcagtcattt
     1261 tccatgggca atattccagt ggccaccctc agccagggct ccagtcctgt gactgtccac
     1321 acactgcctt ctggccctca gctcttccgc tatgccacag tggtctcctc tgccaagagc
     1381 agctcaccag acacagtgac catccaccct tcatctagct tggcgctgct gagctctact
     1441 gccatgcagg atgggagtac actgggcaac atgaccacca tggttagccc tgtggaattg
     1501 gtggccatgg agtccggcct aacctcggca attcaggctg ttgaaagcac ctcagaggat
     1561 gggcagacca tcattgagat tgatccagcc ccggacccag aagctgaaga tactgagggc
     1621 aaagcagtca tcttggagac agagctgagg actgaggaga aagttgtggc tgagatggaa
     1681 gaacaccagc atcaagttca caatgtggag attgtggtct tagaggatta gtaatgaact
     1741 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1801 ttagtcatga gctgttgctt gta
//
