LOCUS       sequence-283527-        2045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1401
FEATURES             Location/Qualifiers
     source          1..2045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcaggg atcagtgtct ttcaatgatg tgactgtgga cttcactcag
       61 gaggagtggc agcacctgga tcatgctcag aagactctat atatggatgt gatgttggaa
      121 aactattgcc acctcatctc tgtggggtgt cacatgacca aacctgatgt gatcctcaag
      181 ttggaacgag gagaagagcc atggacatca tttgcaggtc atacctgctt ggaagaaaac
      241 tggaaagctg aagacttttt agtgaaattc aaggaacacc aagagaagta ttctagatca
      301 gttgtaagca tcaaccacaa aaaactggtg aaggagaaga gtaaaatata tgaaaagaca
      361 tttactctag gcaaaaaccc tgtgaattca aaaaatctac ctcctgaata tgatactcat
      421 ggaaggattt tgaaaaatgt ttcagaatta atcatcagta atctaaatcc tgcaagaaag
      481 agacttagtg agtataatgg atatgggaaa tcactcctga gtactaaaca agagactact
      541 catcctgaag tcaaatccca taatcaaagt gccagagctt tcagtcataa tgaagttctt
      601 atgcagtatc agaaaacgga aactccagca cagtcatttg gatataatga ctgtgagaaa
      661 tcattccttc aaaggggagg cctgattaca catagtagac cttacaaagg agaaaaccca
      721 tctgtatata ataaaaaaag aagagcaacc aatattgaaa aaaaacatac atgcaatgaa
      781 tgtgggaaat ctttctgcag gaaatcagta ttgattctgc atcagggaat tcactcagaa
      841 gaaaaaccct atcaatgtca tcaatgtgga aatgcattta gaaggaaatc atatctcatt
      901 gatcatcaga gaactcacac aggagagaaa ccctttgttt gcaatgaatg tggtaagtcc
      961 ttccgcctca agacagccct cactgatcat cagagaacac acacagggga gaaatcgtat
     1021 gaatgtctgc aatgtaggaa tgccttcaga ttgaagtcac acctcattcg tcatcagaga
     1081 actcacacgg gagagaaacc atatgagtgt aatgactgtg ggaagtcctt ccgccagaag
     1141 acaacactct ctctacatca gagaatccat acaggtgaga aaccctacat ttgtaaagaa
     1201 tgtgggaagt cctttcacca gaaggcaaat cttactgtac atcagagaac tcatacaggg
     1261 gaaaagccct atatttgtaa tgaatgtggg aaatccttct cccagaagac aacccttgct
     1321 cttcatgaga aaactcataa tgaggagaaa ccctatattt gtagtgaatg tggaaagtcc
     1381 ttccgccaga agacaaccct tgtagcacat cagagaacac atacagggga gaaatcttat
     1441 gaatgtcctc actgtgggaa ggcctttaga atgaagtcat acctcattga tcatcaccga
     1501 actcacacag gagagaaacc atatgaatgt aatgaatgtg gtaaatcatt cagtcaaaag
     1561 acaaatctca atctacatca gagaattcat acaggggaga aaccctatgt ttgtaatgaa
     1621 tgtgggaagt cctttcgcca gaaagcaacc ctcactgtac atcagaaaat acataccggc
     1681 cagaaatcct atgaatgtcc tcagtgtggg aaagccttta gcaggaagtc atatctcatt
     1741 catcatcaaa gaactcatac gggagagaaa ccatataaat gtagtgaatg tggaaagtgc
     1801 ttccgccaga agacaaatct tattgtacat cagagaactc acacaggtga gaaaccctat
     1861 gtttgtaatg agtgtggtaa gtctttcagt tataagagaa acctcattgt ccatcaaaga
     1921 actcacaagg gagaaaacat tgaaatgcaa tagtaatgaa ctagtccgta acttgaaagt
     1981 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgtctctag tcgaataatt
     2041 aacag
//
