LOCUS       sequence-283584-        4001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1524
FEATURES             Location/Qualifiers
     source          1..4001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattcctt agaccatatg ctgacagatc ctctggaact tggtccgtgt
       61 ggagatggcc atggcacgcg catcatggag gattgcctcc tgggaggcac cagagttagt
      121 ctgcccgagg accttctgga ggatcctgag atcttctttg atgttgtcag cctctcaaca
      181 tggcaggaag tgttaagtga ttctcaacgt gaacacctcc agcagtttct gccccagttt
      241 cctgaagaca gtgctgagca gcagaatgaa ctcatcttag ccttgttcag tggggagaac
      301 ttccgctttg gaaaccctct gcacattgcc cagaagcttt tccgagacgg acactttaac
      361 cccgaggtgg tcaagtaccg gcagttatgc ttcaagtcac agtacaagcg ctacctcaac
      421 tcccagcagc agtatttcca tcggctgctg aagcaaattc ttgcttcccg gagtgatctg
      481 ctggagatgg cccggcggag tggccccgcc cttcccttcc ggcagaaacg cccttcacca
      541 tcccgcacac ctgaggagcg ggagtggcgg acccagcagc gctacttgaa ggtcttaagg
      601 gaagtgaaag aggagtgtgg tgacacagcc ctgtcatctg atgaagagga tctcagctca
      661 tggcttccga gctctccagc acgttctcct agtcctgcgg tgcccctgcg ggtggtgccc
      721 acactttcaa ccacggatat gaaaactgca gataaagtag aactggggga cagtgacctg
      781 aagataatgt taaagaagca ccacgagaag cggaaacatc agccagatca cccggacctt
      841 ttgacagggg acctgactct caatgacatc atgactcgag taaatgctgg caggaagggc
      901 tctctggcag ccttatatga cttggctgtc cttaaaaaaa aggttaagga aaaagaggaa
      961 aagaagaaga agaaaataaa aacgatcaaa tcagaggcag aggacctggc cgagccgcta
     1021 agcagtactg aaggggtcgc acctctctca caggccccct ctccgctggc aattcctgct
     1081 atcaaggaag agccccttga agacctcaag ccttgccttg gaatcaatga aatatcttcc
     1141 agcttcttct ctcttctatt agagatcttg ctgctggaga gtcaggctag ccttcctatg
     1201 ctagaggagc gagttttgga ttggcagtca tcgccagcca gctccctcaa cagctggttc
     1261 tctgcggccc ccaactgggc tgagttggta ctaccagccc tgcagtatct tgctggagaa
     1321 agtcgagctg ttccttccag tttctctcca tttgttgaat tcaaagagaa aacccagcag
     1381 tggaagttgc ttggccaatc ccaagataat gaaaaggaat tagctgccct cttccagcta
     1441 tggctagaga ccaaagatca ggccttctgt aagcaagaaa atgaagacag ctcagatgcc
     1501 acaacacctg tccctcgggt aagaactgac tatgtggtgc gtcccagcac gggggaggag
     1561 aaacgggttt ttcaggagca ggagcgttac aggtatagcc aaccccataa ggcgttcacc
     1621 tttcgcatgc acggctttga gtctgtggtg gggccagtga agggcgtgtt tgacaaggag
     1681 acctcgctca acaaggctcg ggagcactcc ctgctgcgct ccgaccggcc tgcctacgtc
     1741 accattctgt ctcttgttcg ggacgctgcg gctcgactgc ctaatggaga aggcacacgg
     1801 gcagagatct gtgaactgct taaggactcc cagtttcttg caccagatgt caccagcact
     1861 caggtaaata cagtagtgag tggtgcactg gatcggctac attacgaaaa agatccctgt
     1921 gtgaaatacg acattggacg aaagctgtgg atctacctgc atcgtgaccg gagtgaagaa
     1981 gagtttgagc ggattcacca agcacaagca gctgcagcta aagccagaaa agctcttcag
     2041 caaaaaccca agcccccatc caaggtgaag tccagtagca aggagagctc cataaaggtc
     2101 cttagcagtg gcccttctga gcagagccag atgagcctca gtgactccag tatgccaccc
     2161 accccagtca cacctgtaac ccccaccaca ccagcattgc ccgccattcc catctcccct
     2221 ccacctgtat cggcagtgaa caaaagcggc ccttccacag tctcagaacc agctaagtct
     2281 agctcgggtg ttcttctggt gtcttcacca acaatgccac atctgggaac aatgctttcc
     2341 ccagcttcca gccagactgc acccagttct caggctgccg cccgggtcgt gagccactct
     2401 ggctctgctg gactgtctca ggtgcgagtg gtggcccagc ctagccttcc tgctgttccc
     2461 cagcagtcgg gagggccggc acagacattg ccacagatgc cagcaggacc gcagatccgg
     2521 gttccagcca ctgccacaca gaccaaagta gtgccccaga cagtaatggc cactgtgccc
     2581 gtcaaagcgc agactacggc agccactgtg cagcggcctg gacccgggca gacagggctc
     2641 acggtgacaa gtctccctgc cacagccagc cctgtgagta agccagccac gagttctcct
     2701 gggacctctg ctcccagtgc ctccacggct gccgtcattc aaaatgtcac aggacagaac
     2761 atcatcaagc aggtggcaat cactgggcag cttggtgtga agccccaaac aggcaacagc
     2821 attccactca cagccactaa cttccgcatc cagggtaagg atgtattgcg tctgccgccc
     2881 tcttccatca ccacagatgc caagggccag acggttctgc gaatcactcc ggacatgatg
     2941 gccacattgg ccaagtccca ggttaccaca gtcaaattga cccaggacct cttcgggaca
     3001 ggaggcaaca ctacaggcaa aggcatctct gccaccttac acgtcacttc caatccagta
     3061 catgcagctg atagccctgc caaggccagt tcagccagtg ccccttcatc cactccaaca
     3121 ggtaccactg tggtcaaagt gactcctgac ctcaagccaa cagaagcctc aagttcggct
     3181 tttcgcttga tgccagctct tggcgtgagt gtggctgacc agaagggaaa aagcacagtg
     3241 gcctcttcag aagcaaaacc agctgccacg atccgcatcg tgcagggact gggagtgatg
     3301 cctcccaaag caggccagac catcaccgtt gcaacccacg ccaagcaagg ggcctcggtg
     3361 gccagtgggt ctggaactgt ccatacttca gcggtgtcct tacccagtat gaatgctgct
     3421 gtgtccaaga ctgtagctgt ggcttctggg gctgcaagca cccccatcag catcagcaca
     3481 ggagccccca ccgtgcggca ggtccctgtc agcaccacgg ttgtgtccac gtcccaggct
     3541 gggaagttgc ctacacggat cacagttccc ctctctgtga tcagccagcc aatgaagggc
     3601 aagagcgtgg tcacagcccc catcatcaaa ggcaaccttg gagccaacct cagtgggttg
     3661 ggccgcaaca tcatcctcac aactatgcca gcaggcacta agctcattgc tggcaataag
     3721 cctgttagtt tcctcactgc tcagcagttg cagcagcttc agcagcaagg tcaggccaca
     3781 caggtgcgca tccagactgt ccctgcatcc catctccaac agggaacagc ttctggctcc
     3841 tccaaagcag tctccactgt tgttgtgact acagctccgt ctcctaaaca ggcacctgag
     3901 caacaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3961 tcttgtggaa aggacgacac agagtgtagt caattaacgc c
//
