LOCUS       sequence-283596-        1871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1538
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1551..1560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgaacaaccg aaaggaagat atggaaatca cgtcccacta ccggcacctg
       61 ctgcgggagc tcaacgagca gaggcagcac ggcgtcctgt gcgacgtctg cgtggtcgtg
      121 gagggcaagg tcttcaaggc gcacaagaac gtcctgctgg gcagcagccg ctacttcaag
      181 acgctctact gccaggtgca gaagacgtcg gagcaggcca cggtcacgca cctggacatc
      241 gtcacggccc agggcttcaa ggccatcatc gacttcatgt actcagcgca cctggcgctc
      301 accagcagga acgtcatcga ggtgatgtca gccgccagct tcctgcagat gacggacatc
      361 gtgcaagcct gccacgactt catcaaggcg gcgctggaca tcagcatcaa gtcggacgcc
      421 tcagatgagc ttgcggagtt cgagatcggc gcctcgtcca gcagcagcac ggaagctctc
      481 atctcggccg tgatggctgg gaggagcatc tccccgtggc tggcacggcg aacgagtcct
      541 gccaattctt ccggagactc ggccatcgcc agctgtcacg acggagggag cagctacggg
      601 aaagaggatc aggagcccaa ggccgatggc cctgatgatg tttcttcaca gcctctatgg
      661 cctggagacg tgggctacgg gcctctgcgc atcaaggaag agcaggtttc accgtctcag
      721 tacggaggga gcgagctgcc ttctgccaag gacggtgcag tacagaactc tttctcagag
      781 cagagtgctg gtgatgcctg gcagcccacg ggccgaagga agaatcggaa aaacaaagag
      841 accgtccggc acatcacaca gcaggtggaa gatgacagcc gggccagctc cccggtgccg
      901 tccttcctgc cgacgtcggg gtggccgttc agcagccgag actcaaatgc ggacctgtcc
      961 gtcaccgaag ccagcagctc cgacagccga ggagagaggg ccgagctcta tgcacaggtg
     1021 gaggagggtc tcctgggagg agaagccagc tatctgggcc ctcccctcac cccagagaag
     1081 gacgacgccc tgcatcaggc caccgcggtg gccaacctgc gcgcggcgct catgagtaag
     1141 aacagcctgc tgtcgctgaa ggccgacgtg ctgggggatg acggctccct gctgttcgag
     1201 tacctgccca gaggggccca ctcgctgtcc ctgaatgagt tcacggtgat caggaagaag
     1261 ttcaagtgtc cgtactgcag cttctcggcc atgcaccagt gcatcctcaa gcgacacatg
     1321 cgctcgcaca cgggagagcg gccctacccc tgcgagatct gcgggaagaa gttcacgcgg
     1381 cgcgagcaca tgaagcgcca cacgctggtc cacagcaagg acaagaagta tgtgtgcaag
     1441 gtgtgcagcc gcgtcttcat gtccgccgcc agcgtgggca tcaggcatgg ctccaggcgc
     1501 cacggtgtgt gcaccgactg tgctggccgc ggcatggccg ggcccctgga ccatggcggc
     1561 ggaggcggcg agggctctcc agaggcgctg ttcccaggcg acgggcccta tctggaggac
     1621 cctgaggacc cacgagggga ggcggaggag ctgggcgagg acgacgaggg cctggcccct
     1681 gaggatgcgc tgttggcgga cgacaaggat gaggaagact cgccgcggcc gcgcagcccc
     1741 ccaggaggcc ctgacaagga cttcgcctgg ctctcctagt aatgaactag tccgtaactt
     1801 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattt cctcagccaa
     1861 gaccattcta t
//
