LOCUS       sequence-283611-        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1598
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctggtgc tcagactctg ttgacgttca gggatgtggc catagaattc
       61 tccctggagg agtggaaatg cctggacctc gctcagcaga atttgtacag ggatgtgatg
      121 ttggagaact acagaaactt gttctccgtt ggtctcactg tctgtaagcc aggcctgatc
      181 acctgcctgg agcaacgaaa agagccctgg aatgtgaaga gacaggaggc agcagacgga
      241 catccagaga tgggatttca ccatgctact caggcttgtc ttgaactcct gggctcaagc
      301 gatctgcctg cctcagcctc ccaaagtgct gggattacag gtgtgaacca ccgtgcccag
      361 ccaggcctca atgtttctgt ggacaaattc actgctatgt cttctcattt tacccaagac
      421 cttctgccag agcagggcat acaagatgca ttcccaaaaa gaatactgag aggatatgga
      481 aattgtggcc ttgataattt atatttaagg aaagactggg aaagtttaga tgagtgtaag
      541 ttgcaaaaag attataatgg acttaaccaa tgttcatcaa ctacccatag caaaatcttt
      601 caatataata aatatgttaa aatctttgat aacttttcaa atttacatag acgtaatata
      661 agtaatactg gagagaaacc tttcaaatgt caagaatgtg gcaaatcctt tcaaatgctc
      721 tcattcctaa ctgaacatca gaaaattcac actggaaaaa aattccaaaa atgtggagaa
      781 tgtggcaaaa cctttatcca gtgctcacac tttactgaac ctgagaacat tgacactgga
      841 gagaaacctt acaagtgtca agaatgtaac aacgtcatta aaacttgctc agtccttact
      901 aaaaatagaa tttatgccgg aggggaacat tacagatgtg aagaatttgg caaagtattt
      961 aaccagtgct cccaccttac tgaacatgag catggtactg aggaaaaacc ctgcaaatat
     1021 gaagagtgca gcagtgtctt tatctcttgc tcaagccttt ctaatcaaca gatgattctt
     1081 gctggagaga agctctccaa atgtgaaaca tggtacaaag gttttaacca cagcccaaat
     1141 ccttccaaac accagagaaa tgagattgga gggaaacctt tcaaatgtga ggaatgtgac
     1201 agcatcttca agtggttctc agaccttact aaacataaga gaattcacac tggtgagaaa
     1261 ccatacaaat gtgacgaatg tgggaaagcc tatacacagt cctcacacct cagtgaacac
     1321 aggaggattc acaccggaga gaaaccctac caatgtgaag aatgtgggaa ggtcttcaga
     1381 acttgctcaa gcctttctaa ccataagaga actcattctg aagaaaaacc ctacacgtgt
     1441 gaagaatgtg gcaacatctt taagcagtta tcagacctca ctaagcataa gaaaacccat
     1501 actggagaga agccctacaa atgtgacgaa tgtggaaaaa actttaccca gtcctccaac
     1561 cttattgtac ataagagaat tcatactgga gagaaaccct acaagtgtga agaatgtggc
     1621 agagtcttca tgtggttctc agacattacc aaacataaga aaacccatac tggagagaaa
     1681 ccctacaaat gtgacgaatg tggaaaaaac tttacccagt cctcaaacct tattgtacat
     1741 aagagaattc atactggaga gaaaccctac aagtgtgaaa agtgtggcaa agccttcacc
     1801 cagttctcac acctgactgt acatgaaagc attcatactt agtaatgaac tagtccgtaa
     1861 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tccctcgtac
     1921 tcccgcttcc atga
//
