LOCUS       sequence-283617-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1637
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcgagca taatctcctg aatcccgggt ttgtggggcc gctggtaaac
       61 atccacacgg gagacacctt ctacttcccc aacttccgcg cgtccggggc gcagcttccc
      121 gggctgcctt cgctgtccta cccacgccgc gacaacgtgt gctccctgtc ctggccgtcg
      181 gcggagccgt gcaatggcta cccgcagccc tacctcggca gcccagtgtc tctcaaccct
      241 cccttcggcc gcacgtgcga gctggcgcgc gtggaggacg gcaagggtta ctaccgcgag
      301 ccgtgcgccg agggtggcgg cgggggcctg aagcgtgagg agcgcgggcg cgacccggga
      361 gccgggcccg gggcagcgct gctcccgctg gagccgtcgg ggccgcctgc gctcggcttc
      421 aagtacgact acgcggcggg cggcggcggt ggcgacggcg gcggcggcgc aggacctccg
      481 cacgacccgc cctcctgcca gtcgctggaa tccgactcca gttcgtccct gctcaacgag
      541 ggcaacaagg gcgccggcgc aggcgacccc ggcagcttgg tatcgccgtt gaaccccggc
      601 ggcgggctct cggccagcgg cgcgccctgg tacccgatca acagccgctc tcggaagaag
      661 cgcaagccct attcgaagtt gcaactggca gagctggagg gcgagtttct ggtcaacgag
      721 ttcatcacac gccagcgccg gagggaactc tcagaccgct tgaatcttag tgaccagcag
      781 gtcaagatct ggtttcagaa ccggagaatg aaaaagaaaa gacttctgtt gagggagcaa
      841 gctctctcct tcttttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      901 ctttatatat cttgtggaaa ggacgaaatc ggctgaaggt ctaaggacat
//
