LOCUS       sequence-283698-        1559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1865
FEATURES             Location/Qualifiers
     source          1..1559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggttcactc cagcatgggg gctccagaaa taagaatgtc taagcccctg
       61 gaggccgaga agcaaggtct ggactcccca tcagagcaca cagacaccga aagaaatgga
      121 ccagacacta atcatcagaa cccccaaaat aagacctccc cattctccgt gtccccaact
      181 ggccccagta caaaggtggg cattctctct ggcctccact taacattctg gggtcccgga
      241 ccctgcctct ctccccccca gatcaaggct gaagacccca gtggcgattc agccccagca
      301 gcacccctgc cccctcagcc ggcccagcct catctgcccc aggcccaact catgttgacg
      361 ggcagccagc tagctgggga catacagcag ctcctccagc tccagcagct ggtgcttgtg
      421 ccaggccacc acctccagcc acctgctcag ttcctgctac cgcaggccca gcagagccag
      481 ccaggcctgc taccgacacc aaatctattc cagctacctc agcaaaccca gggagctctt
      541 ctgacctccc agccccgggc cgggcttccc acacagcccc ccaaatgctt ggagccacca
      601 tcccaccccg aggagcccag tgatctggag gagctggagc aattcgcccg caccttcaag
      661 caacgccgca tcaagctggg cttcacgcag ggtgatgtgg gcctggccat gggcaagctc
      721 tacggcaacg acttcagcca gacgaccatt tcccgcttcg aggccctcaa cctgagcttc
      781 aagaacatgt gcaaactcaa gcccctcctg gagaagtggc tcaacgatgc agagactatg
      841 tctgtggact caagcctgcc cagccccaac cagctgagca gccccagcct gggtttcgac
      901 ggcctgcccg gccggagacg caagaagagg accagcatcg agacaaacgt ccgcttcgcc
      961 ttagagaaga gttttctagc gaaccagaag cctacctcag aggagatcct gctgatcgcc
     1021 gagcagctgc acatggagaa ggaagtgatc cgcgtctggt tctgcaaccg gcgccagaag
     1081 gagaaacgca tcaacccctg cagtgcggcc cccatgctgc ccagcccagg gaagccggcc
     1141 agctacagcc cccatatggt cacaccccaa gggggcgcgg ggaccttacc gttgtcccaa
     1201 gcttccagca gtctgagcac aacagttact accttatcct cagctgtggg gacgctccac
     1261 cccagccgga cagctggagg gggtgggggc gggggcgggg ctgcgccccc cctcaattcc
     1321 atcccctctg tcactccccc acccccggcc accaccaaca gcacaaaccc cagccctcaa
     1381 ggcagccact cggctatcgg cttgtcaggc ctgaacccca gcacgggccc tggcctctgg
     1441 tggaaccctg ccccttacca gccttagtaa tgaactagtc cgtaacttga aagtatttcg
     1501 atttcttggc tttatatatc ttgtggaaag gacgagtttc aacaaatatt tgtctataa
//
