LOCUS       sequence-283722-        1907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2015
FEATURES             Location/Qualifiers
     source          1..1907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttatga tgactccgtg aagaaagaag attgttttga tggtgatcat
       61 acctttgagg acataggact tgcagctggc cgaagccaac gagagaaaaa acgttcttac
      121 aaagattttt taagggaaga ggaagaaatt gctgctcagg tcaggaattc ttccaagaag
      181 aagttgaagg atagtgaact ttacttcttg gggacggaca cacacaagaa gaagaggaag
      241 cactcctctg atgattacta ctatggagat atttcgtctt tggaatcgtc acagaagaaa
      301 aagaaaaagt ccagcccaca gtctactgat acagctatgg acctgttgaa agctatcact
      361 tccccactgg cagcaggctc caagccctcc aaaaagactg gggagaaatc ctctggctct
      421 tcaagccatt cggagagtaa aaaggagcac cacaggaaga aagtcagtgg aagcagtggg
      481 gaactacccc tagaggatgg tggctcccac aaatcgaaaa aaatgaaacc tctctatgtg
      541 aacacagaga cactgaccct tcgggagcct gatggtttaa aaatgaaact tattctgtca
      601 ccaaaggaga agggaagcag ctctgttgat gaggagtctt ttcaatatcc ctcccaacaa
      661 gcgactgtga aaaaatcctc aaagaaatca gctcgggatg agcagggtgc tttactccta
      721 ggacatgagt tacagagctt tctgaaaaca gcccggaaaa agcacaagtc atcctcagac
      781 gcacattcat ctcctggccc tgaaggctgt gggtctgacg cctcccagtt cgcagagtcc
      841 cacagtgcta accttgatct ttcagggctt gaacctattc tggtagaatc agactcatcc
      901 tctggtgggg aactagaggc tggggagtta gtgatagatg attcttaccg agaaatcaag
      961 aagaaaaaga agtcaaagaa gagcaaaaag aagaaagaca aggagaagca taaagagaag
     1021 cgacactcca agtccaagag aagtttagga ctttctgccg tgccagtggg agaggtcaca
     1081 gtgacatctg gccctcctcc cagcatccca tacgctggag cagcagcacc tcccctgcca
     1141 cttcctggcc tccacacaga tgggcatagt gaaaaaaaaa agaaaaaaga agagaaggac
     1201 aaagagagag agagaggaga aaagccaaaa aagaagaaca tgtcggccta ccaggtgttc
     1261 tgtaaagagt atcgcgtgac cattgtggct gaccatccag gtatagattt tggggaactt
     1321 agtaaaaaac tggctgaggt gtggaagcaa ttaccagaaa aagacaaact gatttggaag
     1381 caaaaagctc agtatctgca gcacaaacag aacaaagcag aagccacaac tgtgaaaagg
     1441 aaagcatcca gctcagaagg ttccatgaaa gtcaaagcct cttctgtagg agtactgtca
     1501 ccccagaaga agtccccacc caccaccatg ctgttaccag cctcaccagc caaagcccct
     1561 gagacagagc ccattgatgt tgctgctcat cttcagctgt tgggagagtc cctaagcctc
     1621 attggacacc gtctgcagga aactgagggt atggtggctg tgtctggcag tttgtcagtg
     1681 cttctggatt ccattatctg tgcccttggc cccttggcat gtctcaccac acaactacct
     1741 gaattgaatg gctgtcccaa acaggtcttg tcaaacacat tagacaacat tgcttacatc
     1801 atgccgggac tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1861 tatatatctt gtggaaagga cgagacacct ccagatcgag ctgttgt
//
