LOCUS       sequence-284196-        6812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2429
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             259..276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             280..297
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcccataa cgcgggcgcc gcggccgccg ccggcaccca cagcgccaag
       61 agcggcggct ccgaggcggc tctcaaggag ggtggaagcg ccgccgcgct gtcctcctcc
      121 tcctcctcct ccgcggcggc agcggcggca tcctcttcct cctcgtcggg cccgggctcg
      181 gccatggaga cggggctgct ccccaaccac aaactgaaaa ccgttggcga agcccccgcc
      241 gcgccgcccc accagcagca ccaccaccac caccatgccc accaccacca ccaccatgcc
      301 caccacctcc accaccacca cgcactacag cagcagctaa accagttcca gcagcagcag
      361 cagcagcagc aacagcagca gcagcagcag cagcaacagc aacatcccat ttccaacaac
      421 aacagcttgg gcggcgcggg cggcggcgcg cctcagcccg gccccgacat ggagcagccg
      481 caacatggag gcgccaagga cagtgctgcg ggcggccagg ccgacccccc gggcccgccg
      541 ctgctgagca agccgggcga cgaggacgac gcgccgccca agatggggga gccggcgggc
      601 ggccgctacg agcacccggg cttgggcgcc ctgggcacgc agcagccgcc ggtcgccgtg
      661 cccgggggcg gcggcggccc ggcggccgtc ccggagttta ataattacta tggcagcgct
      721 gcccctgcga gcggcggccc cggcggccgc gctgggcctt gctttgatca acatggcgga
      781 caacaaagcc ccgggatggg gatgatgcac tccgcctccg ccgccgccgc cggggccccc
      841 ggcagcatgg accccctgca gaactcccac gaagggtacc ccaacagcca gtgcaaccat
      901 tatccgggct acagccggcc cggcgcgggc ggcggcggcg gcggcggcgg cggaggagga
      961 ggaggcagcg gaggaggagg aggaggagga ggagcaggag caggaggagc aggagcggga
     1021 gctgtggcgg cggcggccgc ggcggcggcg gcagcagcag gaggcggcgg cggcggcggc
     1081 tatgggggct cgtccgcggg gtacggggtg ctgagctccc cccggcagca gggcggcggc
     1141 atgatgatgg gccccggggg cggcggggcc gcgagcctca gcaaggcggc cgccggctcg
     1201 gcggcggggg gcttccagcg cttcgccggc cagaaccagc acccgtcggg ggccaccccg
     1261 accctcaatc agctgctcac ctcgcccagc cccatgatgc ggagctacgg cggcagctac
     1321 cccgagtaca gcagccccag cgcgccgccg ccgccgccgt cgcagcccca gtcccaggcg
     1381 gcggcggcgg gggcggcggc gggcggccag caggcggccg cgggcatggg cttgggcaag
     1441 gacatgggcg cccagtacgc cgctgccagc ccggcctggg cggccgcgca acaaaggagt
     1501 cacccggcga tgagccccgg cacccccgga ccgaccatgg gcagatccca gggcagccca
     1561 atggatccaa tggtgatgaa gagacctcag ttgtatggca tgggcagtaa ccctcattct
     1621 cagcctcagc agagcagtcc gtacccagga ggttcctatg gccctccagg cccacagcgg
     1681 tatccaattg gcatccaggg tcggactccc ggggccatgg ccggaatgca gtaccctcag
     1741 cagcagatgc cacctcagta tggacagcaa ggtgtgagtg gttactgcca gcagggccaa
     1801 cagccatatt acagccagca gccgcagccc ccgcacctcc caccccaggc gcagtatctg
     1861 ccgtcccagt cccagcagag gtaccagccg cagcaggaca tgtctcagga aggctatgga
     1921 actagatctc aacctcctct ggcccccgga aaacctaacc atgaagactt gaacttaata
     1981 cagcaagaaa gaccatcaag tttaccagat ctgtctggct ccattgatga cctccccacg
     2041 ggaacggaag caactttgag ctcagcagtc agtgcatccg ggtccacgag cagccaaggg
     2101 gatcagagca acccggcgca gtcgcctttc tccccacatg cgtcccctca tctctccagc
     2161 atcccggggg gcccatctcc ctctcctgtt ggctctcctg taggaagcaa ccagtctcga
     2221 tctggcccaa tctctcctgc aagtatccca ggtagtcaga tgcctccgca gccacccggg
     2281 agccagtcag aatccagttc ccatcccgcc ttgagccagt caccaatgcc acaggaaaga
     2341 ggttttatgg caggcacaca aagaaaccct cagatggctc agtatggacc tcaacagaca
     2401 ggaccatcca tgtcgcctca tccttctcct gggggccaga tgcatgctgg aatcagtagc
     2461 tttcagcaga gtaactcaag tgggacttac ggtccacaga tgagccagta tggaccacaa
     2521 ggtaactact ccagaccccc agcgtatagt ggggtgccca gtgcaagcta cagcggccca
     2581 gggcccggta tgggtatcag tgccaacaac cagatgcatg gacaagggcc aagccagcca
     2641 tgtggtgctg tgcccctggg acgaatgcca tcagctggga tgcagaacag accatttcct
     2701 ggaaatatga gcagcatgac ccccagttct cctggcatgt ctcagcaggg agggccagga
     2761 atggggccgc caatgccaac tgtgaaccgt aaggcacagg aggcagccgc agcagtgatg
     2821 caggctgctg cgaactcagc acaaagcagg caaggcagtt tccccggcat gaaccagagt
     2881 ggacttatgg cttccagctc tccctacagc cagcccatga acaacagctc tagcctgatg
     2941 aacacgcagg cgccgcccta cagcatggcg cccgccatgg tgaacagctc ggcagcatct
     3001 gtgggtcttg cagatatgat gtctcctggt gaatccaaac tgcccctgcc tctcaaagca
     3061 gacggcaaag aagaaggcac tccacagccc gagagcaagt caaagaagtc cagctcctcc
     3121 accactactg gggagaagat cacgaaggtg tacgagctgg ggaatgagcc agagagaaag
     3181 ctctgggtcg accgatacct caccttcatg gaagagagag gctctcctgt ctcaagtctg
     3241 cctgccgtgg gcaagaagcc cctggacctg ttccgactct acgtctgcgt caaagagatc
     3301 gggggtttgg cccaggttaa taaaaacaag aagtggcgtg agctggcaac caacctaaac
     3361 gttggcacct caagcagtgc agcgagctcc ctgaaaaagc agtatattca gtacctgttt
     3421 gcctttgagt gcaagatcga acgtggggag gagcccccgc cggaagtctt cagcaccggg
     3481 gacaccaaaa agcagcccaa gctccagccg ccatctcctg ctaactcggg atccttgcaa
     3541 ggcccacaga ccccccagtc aactggcagc aattccatgg cagaggttcc aggtgacctg
     3601 aagccaccta ccccagcctc cacccctcac ggccagatga ctccaatgca aggtggaaga
     3661 agcagtacaa tcagtgtgca cgacccattc tcagatgtga gtgattcatc cttcccgaaa
     3721 cggaactcca tgactccaaa cgccccctac cagcagggca tgagcatgcc cgatgtgatg
     3781 ggcaggatgc cctatgagcc caacaaggac ccctttgggg gaatgagaaa agtgcctgga
     3841 agcagcgagc cctttatgac gcaaggacag atgcccaaca gcagcatgca ggacatgtac
     3901 aaccaaagtc cctccggagc aatgtctaac ctgggcatgg ggcagcgcca gcagtttccc
     3961 tatggagcca gttacgaccg aaggcatgaa ccttatgggc agcagtatcc aggccaaggc
     4021 cctccctcgg gacagccgcc gtatggaggg caccagcccg gcctgtaccc acagcagccg
     4081 aattacaaac gccatatgga cggcatgtac gggcccccag ccaagcgcca cgagggcgac
     4141 atgtacaaca tgcagtacag cagccagcag caggagatgt acaaccagta tggaggctcc
     4201 tactcgggcc cggaccgcag gcccatccag ggccagtacc cgtatcccta cagcagggag
     4261 aggatgcagg gcccggggca gatccagaca cacggaatcc cgcctcagat gatgggcggc
     4321 ccgctgcagt cgtcctccag tgaggggcct cagcagaata tgtgggcagc acgcaatgat
     4381 atgccttatc cctaccagaa caggcagggc cctggcggcc ctacacaggc gcccccttac
     4441 ccaggcatga accgcacaga cgatatgatg gtacccgatc agaggataaa tcatgagagc
     4501 cagtggcctt ctcacgtcag ccagcgtcag ccttatatgt cgtcctcagc ctccatgcag
     4561 cccatcacac gcccaccaca gccgtcctac cagacgccac cgtcactgcc aaatcacatc
     4621 tccagggcgc ccagcccagc gtccttccag cgctccctgg agaaccgcat gtctccaagc
     4681 aagtctcctt ttctgccgtc tatgaagatg cagaaggtca tgcccacggt ccccacatcc
     4741 caggtcaccg ggccaccacc ccaaccaccc ccaatcagaa gggagatcac ctttcctcct
     4801 ggctcagtag aagcatcaca accagtcttg aaacaaaggc gaaagattac ctccaaagat
     4861 atcgttactc ctgaggcgtg gcgtgtgatg atgtccctta aatcaggtct tttggctgag
     4921 agtacgtggg ctttggacac tattaatatt cttctgtatg atgacagcac tgttgctact
     4981 ttcaatctct cccagttgtc tggatttctc gaacttttag tcgagtactt tagaaaatgc
     5041 ctgattgaca tttttggaat tcttatggaa tatgaagtgg gagaccccag ccaaaaagca
     5101 cttgatcaca acgcagcaag gaaggatgac agccagtcct tggcagacga ttctgggaaa
     5161 gaggaggaag atgctgaatg tattgatgac gacgaggaag acgaggagga tgaggaggaa
     5221 gacagcgaga agacagaaag cgatgaaaag agcagcatcg ctctgactgc cccggacgcc
     5281 gctgcagacc caaaggagaa gcccaagcaa gccagtaagt tcgacaagct gccaataaag
     5341 atagtcaaaa agaacaacct gtttgttgtt gaccgatctg acaagttggg gcgtgtgcag
     5401 gagttcaata gtggccttct gcactggcag ctcggcgggg gtgacaccac cgagcacatt
     5461 cagactcact ttgagagcaa gatggaaatt cctcctcgca ggcgcccacc tcccccctta
     5521 agctccgcag gtagaaagaa agagcaagaa ggcaaaggcg actctgaaga gcagcaagag
     5581 aaaagcatca tagcaaccat cgatgacgtc ctctctgctc ggccaggggc attgcctgaa
     5641 gacgcaaacc ctgggcccca gaccgaaagc agtaagtttc cctttggtat ccagcaagcc
     5701 aaaagtcacc ggaacatcaa gctgctggag gacgagccca ggagccgaga cgagactcct
     5761 ctgtgtacca tcgcgcactg gcaggactcg ctggctaagc gatgcatctg tgtgtccaat
     5821 attgtccgta gcttgtcatt cgtgcctggc aatgatgccg aaatgtccaa acatccaggc
     5881 ctggtgctga tcctggggaa gctgattctt cttcaccacg agcatccaga gagaaagcga
     5941 gcaccgcaga cctatgagaa agaggaggat gaggacaagg gggtggcctg cagcaaagat
     6001 gagtggtggt gggactgcct cgaggtcttg agggataaca cgttggtcac gttggccaac
     6061 atttccgggc agctagactt gtctgcttac acggaaagca tctgcttgcc aattttggat
     6121 ggcttgctgc actggatggt gtgcccgtct gcagaggcac aagatccctt tccaactgtg
     6181 ggacccaact cggtcctgtc gcctcagaga cttgtgctgg agaccctctg taaactcagt
     6241 atccaggaca ataatgtgga cctgatcttg gccactcctc catttagtcg tcaggagaaa
     6301 ttctatgcta cattagttag gtacgttggg gatcgcaaaa acccagtctg tcgagaaatg
     6361 tccatggcgc ttttatcgaa ccttgcccaa ggggacgcac tagcagcaag ggccatagct
     6421 gtgcagaaag gaagcattgg aaacttgata agcttcctag aggatggggt cacgatggcc
     6481 cagtaccagc agagccagca caacctcatg cacatgcagc ccccgcccct ggaaccacct
     6541 agcgtagaca tgatgtgcag ggcggccaag gctttgctag ccatggccag agtggacgaa
     6601 aaccgctcgg aattcctttt gcacgagggc cggttgctgg atatctcgat atcagctgtc
     6661 ctgaactctc tggttgcatc tgtcatctgt gatgtactgt ttcagattgg gcagttatag
     6721 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     6781 aaggacgatc gataagcaga atccgccgaa tc
//
