LOCUS       sequence-284331-        1847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0555
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1142..1150
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgttggagaa ctatagcaac ctcgtgtcag tggggtatca agccagcaaa
       61 ccagatgcac tcttcaagtt ggaacaagga gagccatgga cagtagaaaa tgaaatccac
      121 agccaaatct gtccagaaat caagaaagtt gacaatcatc tacagatgca ctcacaaaag
      181 caaagatgtc tgaagagagt ggaacaatgc cataaacata atgcatttgg aaacatcatt
      241 catcagagga aaagtgattt tcctttaagg caaaatcatg atacatttga cttacatggg
      301 aaaatactga aatcaaattt aagtttagtc aaccagaaca aaaggtatga aatcaagaat
      361 tctgtggggg ttaatggaga tgggaaatcc ttccttcatg ccaagcatga acaatttcat
      421 aatgaaatga acttccccga aggtggaaat tctgtgaata caaattcaca attcattaag
      481 catcagcgaa ctcaaaacat agataaaccc catgtatgca ctgagtgtgg gaaggctttc
      541 ctcaagaagt ctcgcctcat ctatcatcag agagttcaca ctggggagaa acctcatgga
      601 tgcagtatat gtgggaaagc cttctccaga aagtccgggc tcactgaaca ccagagaaac
      661 cacacaggag agaaacccta tgaatgcact gaatgtgaca aagcattccg ctggaaatca
      721 cagctcaatg cacaccagaa aattcataca ggagagaagt catatatatg cagtgattgt
      781 ggaaaaggct tcatcaagaa gtctcggctc attaatcatc agagagttca tacaggagag
      841 aaaccacatg gatgcagcct gtgtgggaag gccttctcca aaaggtccag gctcactgaa
      901 caccagagaa ctcatacagg agagaaaccc tatgaatgca ctgaatgtga caaagcattc
      961 cgctggaaat cacagctcaa tgcacatcag aaagctcaca caggagagaa gtcatatata
     1021 tgccgtgatt gtggaaaagg cttcattcag aagggaaatc tcattgtaca tcagcgaatt
     1081 catactggag aaaaacccta tatatgcaat gaatgtggaa aaggcttcat ccaaaagggc
     1141 aacctcctta ttcatcgacg tactcacact ggagagaaac cctatgtatg caatgaatgt
     1201 gggaaaggct tcagccagaa gacatgttta atatcccatc agagatttca cacaggaaag
     1261 acaccctttg tatgtactga gtgtggaaaa tcctgctcac acaagtcagg tctcattaac
     1321 caccagagaa ttcacacagg agagaaaccc tatacatgca gtgactgtgg gaaagctttc
     1381 agagataaat catgtctcaa cagacatcgg agaactcata caggggagag accgtatgga
     1441 tgctctgatt gtgggaaagc tttctcccac ttgtcatgcc ttgtttatca taagggaatg
     1501 ctgcatgcaa gagagaaatg tgtaggttca gtcaaattgg aaaatccttg ctcagagagt
     1561 catagcttat cacatacacg tgatctcata caggataaag actctgttaa catggtgact
     1621 ctgcagatgc cttctgtggc agctcagacc tcattaacta acagtgcgtt ccaagcagag
     1681 agcaaagtag ccattgtgag ccagcctgtt gccagaagtt cagtctcagc agatagtaga
     1741 atttgcacag aatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1801 tatatatctt gtggaaagga cgacgagcca aaccctcgga cgtaata
//
