LOCUS       sequence-284406-        3932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1233
FEATURES             Location/Qualifiers
     source          1..3932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatctatt cgactttttc agagactggg acttggagca gcagtgtcac
       61 tatgaacaag accgtagtgc acttaaaaaa agggaatggg agcggaggaa tcaagaagtc
      121 cagcaagaag acgatctctt ttcttcaggc tttgatcttt ttggggagcc atacaagaca
      181 aacaaaggtg atgcacttgc caaccgagtc cagaacacgc ttggaaacta tgatgaaatg
      241 aagaatttgc taactaacca ttctaatcag aatcacctag tgggaattcc aaagaattct
      301 gtgccccaga atcccaacaa caaaaatgaa ccaagctttt ttccagaaca aaagaacaga
      361 ataattccac ctcaccagga taatacccat ccttcagcac caatgcctcc accttctgtt
      421 gtgatactga attcaactct aatacacagc aacagaaaat caaaacctga gtggtcacgt
      481 gatagtcata accctagcac tgtactggca agccaggcca gtggtcagcc aaacaagatg
      541 cagactttga cacaggacca gtctcaagcc aaactggaag acttctttgt ctacccagct
      601 gaacagcccc agattggaga agttgaagag tcaaacccat ctgcaaagga agacagtaac
      661 cctaattcta gtggagaaga tgctttcaaa gaaatctttc aatccaattc accggaagaa
      721 tctgaattcg ccgtgcaagc gcctgggtct cccctagtgg cttcctcttt attagctcct
      781 agcagtggcc tttcagttca aaacttccca ccagggcttt actgcaaaac aagcatgggg
      841 cagcaaaagc caactgcata cgtcagaccc atggatggcc aggaccaggc accggacatc
      901 tcaccaacac tgaaaccttc aattgaattt gagaacagct ttgggaatct gtcatttgga
      961 acactcttgg atggaaaacc cagtgcagcc agttcaaaga ctaaactgcc aaagttcacc
     1021 atcctccaaa caagtgaagt aagccttccc agtgatccaa gctgtgttga agaaatcttg
     1081 cgggaatcgc agcatctgac cccaggattc accttacaaa agtggaatga cccaaccacc
     1141 agagcttcta caaagtcagt gtctttcaaa tcgatgcttg aggatgacct gaagctgagc
     1201 agtgatgaag atgaccttga gcctgtgaag accttgacca ctcagtgcac tgccactgag
     1261 ctctaccagg ctgttgaaaa ggcaaaacct aggaataatc ctgtgaaccc acccttggcc
     1321 actccccagc ccccacctgc agtgcaagcc agcgggggtt ctggcagctc cagcgaatcg
     1381 gagagcagct ctgagtcgga ttcagacact gaaagtagca ccactgacag cgaatctaat
     1441 gaggcacctc gtgtggcaac tccagagcct gagccaccct caaccaacaa gtggcaactg
     1501 gataaatggc ttaacaaagt gacatcccag aacaagtctt ttatttgtgg ccaaaatgaa
     1561 acacccatgg agactatttc tctgcctcct ccaatcatcc aaccaatgga agtccagatg
     1621 aaagtgaaga cgaatgccag tcaggtccca gctgaaccca aagaaaggcc tctcctcagt
     1681 ctcattaggg agaaagcccg tccacggccc actcagaaaa ttccagaaac aaaggctttg
     1741 aagcataagt tgtcaacaac tagtgagaca gtgtctcaaa ggacaattgg gaaaaaacag
     1801 cccaaaaaag ttgagaagaa caccagcact gacgagttta cctggcccaa accaaatatt
     1861 accagcagca ctcccaaaga aaaagaaagt gtggagcttc atgacccacc aagaggccgc
     1921 aacaaagcca ctgcccacaa accagcccct aggaaagaac caagacctaa catccctttg
     1981 gctcccgaga agaagaagta cagagggcct ggcaagattg tgccaaagtc tcgggaattc
     2041 attgaaacag attcatctac atctgactcc aacacagatc aggaagagac cctgcaaatc
     2101 aaagtcctgc ctccgtgcat tatttctgga ggtaatactg ccaaatccaa ggaaatctgt
     2161 ggtgccagcc tgaccctcag caccttaatg agtagcagtg gcagcaacaa caacttatcc
     2221 atcagtaatg aagagccaac attttcacct attcctgtca tgcaaactga aatcctgtcc
     2281 cctctgcgag atcatgagaa cctgaaaaac ctctgggtga agattgacct tgacttactc
     2341 tctagagtac ctggccacag ctcactccat gcagcacctg ccaagccaga ccacaaggag
     2401 actgccacaa aacccaagcg tcagacagct gtcacagctg tggagaaacc agcccctaag
     2461 ggcaaacgta agcacaagcc aatagaagtt gcagagaaga tccctgagaa gaagcagcgc
     2521 ctggaggagg ccacaactat ctgcttgctc cctccttgca tctcaccagc cccaccccac
     2581 aagcctccca acactagaga aaataattca tccaggagag caaatagaag aaaggaagaa
     2641 aaactatttc ctcctccact ttccccactg ccagaggacc ctccacgccg cagaaatgtc
     2701 agtggcaata atggtccctt tggtcaagac aaaaacatcg ccatgactgg acaaatcaca
     2761 tctaccaaac ctaagagaac tgaaggcaaa ttctgtgcta ctttcaaagg gatatcggag
     2821 ggagacactc caaaaaaggc atcctctgcc accatcactg tcaccaatac tgctattgcc
     2881 actgctactg tcactgctac tgccattgtc accaccactg tcacagctac tgccaccgcc
     2941 acggccacca ccacaactac taccactacc atttccacca tcacctctac catcactact
     3001 ggcctcatgg atagcagtca cctggagatg acgtcctggg cggctctgcc ccttctatcc
     3061 agcagcagca ctaatgtccg gagacccaag ctcacttttg atgactcggt tcacaatgct
     3121 gattattaca tgcaagaagc taagaagctg aagcacaaag ctgatgcact gttcgagaaa
     3181 tttggcaaag ctgtgaatta tgctgatgcc gccctctcct tcactgaatg tggcaatgcc
     3241 atggaacgcg accctctgga agcaaagtcc ccatacacca tgtactctga gactgtggag
     3301 ctcctcaggt atgcaatgag gctgaagaac tttgcaagtc ccttggcttc ggatggggac
     3361 aaaaagctag cagtactatg ctaccgatgt ttatcactcc tctatttgag aatgtttaag
     3421 ctgaagaagg accatgctat gaagtactcc agatcactga tggaatattt taagcaaaat
     3481 gcttcaaaag tcgcacagat accctctcca tgggtaagca atggaaagaa cactccatcc
     3541 ccagtgtctc tcaacaacgt ctcccccatc aacgcaatgg ggaactgtaa caatggccca
     3601 gtcaccattc cccagcgcat tcaccacatg gctgccagcc acgtcaacat cactagcaat
     3661 gtgttacggg gctatgaaca ctgggatatg gccgacaaac tgacaagaga aaacaaagaa
     3721 ttctttggtg atctggacac gctgatgggg cctctgaccc agcacagcag catgaccaat
     3781 cttgtccgct acgttcgcca aggactgtgt tggctgcgca tcgatgccca cttgttgtag
     3841 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3901 aaggacgatt agtagtcttc gtgtagatag at
//
