LOCUS       sequence-28445-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Zhang R, Min W, Sessa WC
  TITLE     Functional analysis of the human endothelial nitric oxide synthase 
            promoter. Sp1 and GATA factors are necessary for basal transcription
            in endothelial cells.
  JOURNAL   J Biol Chem. 1995 Jun 23. 270(25):15320-6.
  PUBMED    7541039
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Jun 23. 270(25):15320-6."
COMMENT     Sequence Label: pGL2 enhancer- F4 LUC
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    66..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(77..96)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     131..150
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(377..832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(514..535)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     726..745
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcagcagaga gactagggct gaggcagggt acctcccggg ttatgttagc tcagttacag
       61 taccataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      241 ggttttttaa agcaagtaaa acctccacgt accttaatat tacttactta tcatggtagc
      301 ttgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      421 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      481 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      541 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
      601 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
      661 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
      721 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
      781 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
      841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      901 atgtatccgc tca
//
