LOCUS       sequence-284601-        3008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2865
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggccat ggacttgggc tctccctcgc tccctaagaa gagcctgcct
       61 gtccctgggg ccctggagca ggtggccagt cggctgagca gcaaagtggc tgcagaggtt
      121 cctcatggca gcaaacagga gctgcaggac ctcaaggccc agagcctgac cacctgcgag
      181 gtctgcggtg cctgctttga gacccgaaag ggcctgtcca gccacgcgcg ctcccacctg
      241 cggcagctgg gagtggcaga gtcggaaagc agcggcgcac ccatcgacct cctctacgag
      301 cttgtgaagc agaagggtct gcctgacgcc caccttgggc tgcccccagg cctggctaag
      361 aagtccagct cactgaagga ggtggtcgcc ggggcccccc ggcccggctt gctcagcctg
      421 gccaagccct tggatgcccc tgctgtcaac aaagccatca agtcgcctcc cggcttctcg
      481 gccaagggcc tgggccaccc gcccagctct ccactcctca aaaagacacc actggccctg
      541 gcgggctccc ctacccctaa gaatcctgag gacaagagcc cccagctgtc cctgagcccc
      601 cggccggcct ccccaaaggc acagtggcct cagtctgagg atgaggggcc cttgaacctc
      661 actttagata gtgacggggg cagagagctg gactgccagc tgtgcggtgc ctggtttgag
      721 acccgcaagg gcctgtctag ccacgcccgt gcccacctgc gccacctggg cgtcagcgat
      781 ccggacgcca agggatcccc catagacgtg ctccacgggc tcatcaggag ggacggcgtc
      841 cagatccgcc tcccacccag gcgcggcgcc ctggcccacc cggggcggcc gcctcccacc
      901 tccgcggccc tctccttgct tcccccccca ccgccggcca agaaggccaa gctgaaggcc
      961 gcgggtatgg ccagcccctg ggggaagcag gacctctcgg ccgccgcagc cgccggcatt
     1021 ttctgggcct ctgatgtgga gccgtctcct ctcaacctct cctcaggccc agagccagca
     1081 cgagacatcc gctgcgagtt ctgtggtgag ttcttcgaga accgcaaggg cctctcgagc
     1141 cacgcgcgct cccatctgcg gcaaatgggc gtgaccgagt ggtacgtcaa tggctcgccc
     1201 atcgacacgc tgcgggagat cctgaagaga cggacccagt ctcggcctgg tggacctccc
     1261 aacccaccag ggccaagccc aaaagccctg gccaagatga tgggcggcgc aggtcctggc
     1321 agctcactgg aagcccgcag cccctcggac cttcacatct cacccttggc caagaagttg
     1381 ccaccaccac cgggcagccc cctgggccac tcaccaactg cctctcctcc tcctacggcc
     1441 cgaaagatgt tcccaggcct ggctgcaccc tccttgccca agaagctgaa gcctgaacaa
     1501 atacgggtgg agatcaagcg ggagatgctg ccgggggccc ttcatgggga actgcaccca
     1561 tctgagggtc cctggggggc accacgggaa gacatgacac ccctgaacct gtcgtcccgg
     1621 gcagagccgg tgcgcgacat ccgctgtgag ttctgcggcg agttcttcga gaaccgcaag
     1681 ggcctgtcga gtcacgcgcg ctcacacctg cggcagatgg gtgtgaccga gtggtccgtc
     1741 aatggttcgc ccatcgacac actgcgagag atcctcaaga agaagtccaa gccgtgcctc
     1801 atcaagaagg agccaccggc tggagacctg gcccctgccc tggctgagga cgggcctccc
     1861 accgtggccc ctgggcccgt gcagtcccca ctgccgctgt cgcccctggc tggccggcca
     1921 ggcaaaccag gtgcagggcc ggcccaggtt cctcgtgagc tcagcctgac gcccatcact
     1981 ggggccaagc cctcagccac tggctacctg ggctcagtgg cagccaagcg gcccctgcag
     2041 gaggaccgcc tcctcccagc agaggtcaag gccaagacct acatccagac tgaactgccc
     2101 ttcaaggcaa agacccttca tgagaagacc tcccactcct ccaccgaggc ctgctgcgag
     2161 ctgtgtggcc tttactttga aaaccgcaag gccctggcca gccacgcacg ggcacacctg
     2221 cggcagttcg gcgtgaccga gtggtgcgtc aatggctcgc ccatcgagac actgagcgag
     2281 tggatcaaac accggcccca gaaggtgggc gcctaccgca gctacatcca gggcggccgc
     2341 cccttcacca agaagttccg cagtgccggc catggccgtg acagtgacaa gcggccgtcc
     2401 ctggggctgg cacccggggg cctggccgtg gtcggccgca gtgccggagg ggagccaggg
     2461 cccgaggctg gccgggcagc cgacggtggt gagcggcctc tggcagccag cccgccaggc
     2521 accgtgaagg ctgaggagca ccagcggcag aacatcaaca aatttgaacg ccgacaagcc
     2581 cgccctccag atgcctccgc agcccgggga ggcgaggaca ccaatgacct acagcagaag
     2641 ctggaggagg tgcggcaacc cccaccccga gtccggccag tcccctccct ggtgccccgg
     2701 cccccccaga catcacttgt caagttcgtg ggcaacatct acaccctcaa atgcaggttc
     2761 tgtgaggtgg aattccaggg ccccctctcc atccaggaag agtgggtgcg gcacttacag
     2821 cggcacatcc tggagatgaa cttctccaaa gcggaccccc cacctgagga gtcccaggcc
     2881 ccgcaggcac agacagcggc ggcagaggct ccctagtaat gaactagtcc gtaacttgaa
     2941 agtatttcga tttcttggct ttatatatct tgtggaaagg acgataaaga tcatgtcgtg
     3001 atatgctt
//
