LOCUS       sequence-284607-        1778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2901
FEATURES             Location/Qualifiers
     source          1..1778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagtgcc ggcggctttg atccctccga cccagctggt cccccctcaa
       61 cccccaatct ccacgtctgc ttcctcctct ggcaccacca cctccacctc ttcggcgact
      121 tcgtctccgg ctccttccat cggacccccg gcgtcctctg ggccaactct gttccgcccg
      181 gagcccatcg cttcggcggc ggcggcggcg gccacagtca cctctaccgg cggcggcggc
      241 ggcggcggcg gcagcggagg cggcggcggc agcagcggca acggaggcgg cggtggcggc
      301 ggcggcggtg gcagcaactg caaccccaac ctggcggccg cgagcaacgg cagcggcggc
      361 ggcggcggcg gcatcagcgc tggcggcggc gtcgcttcca gcacccccat caacgccagc
      421 accggcagca gcagcagcag cagtagcagc agcagcagca gcagcagtag tagcagcagc
      481 agcagtagca gcagcagctg cggccccctc cccgggaaac ccgtgtactc aaccccgtcc
      541 ccagtggaaa acacccctca gaataatgag tgcaaaatgg tggatctgag gggggccaaa
      601 gtggcttcct tcacggtgga gggctgcgag ctgatctgcc tgccccaggc tttcgacctg
      661 ttcctgaagc acttggtggg gggcttgcat acggtctaca ccaagctgaa gcggctggag
      721 atcacgccgg tggtgtgcaa tgtggaacaa gttcgcatcc tgaggggact gggcgccatc
      781 cagccaggag tgaaccgctg caaactcatc tccaggaagg acttcgagac cctctacaat
      841 gactgcacca acgcaagttc tagacctgga aggcctccta agaggactca aagtgtcacc
      901 tccccagaga actctcacat catgccgcat tctgtccctg gtctcatgtc tcctgggata
      961 attccaccaa caggtctgac agcagccgct gcagcagctg ctgctgctac caatgcagct
     1021 attgctgaag caatgaaggt gaaaaaaatc aaattagaag ccatgagcaa ctatcatgcc
     1081 agtaataacc aacatggagc agactctgaa aacggggaca tgaattcaag tgtcgatgag
     1141 accccgcttt ctacaccaac cgcaagagac agccttgaca aactctctct aactgggcat
     1201 ggacaaccac tgcctccagg ttttccatct ccttttctgt ttcctgatgg actgtcttcc
     1261 atcgagactc ttctgactaa catacagggg ctgttgaaag ttgccataga taatgccaga
     1321 gctcaagaga aacaggtcca actggaaaaa actgagctga agatggattt tttaagggaa
     1381 agagaactaa gggaaacact tgagaagcag ttggctatgg aacaaaagaa tagagccata
     1441 gttcaaaaga ggctaaagaa ggagaagaag gcaaagagaa aattgcagga agcacttgag
     1501 tttgagacga aacggcgtga acaagcagaa cagacgctaa aacaggcagc ttcaacagat
     1561 agtctcaggg tcttaaatga ctctctgacc ccagagatag aggctgaccg cagtggcggc
     1621 agaacagatg ctgaaaggac aatacaagat ggaagactgt atttgaaaac tactgtcatg
     1681 tactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1741 tgtggaaagg acgacacaag gttagattat agctgcat
//
