LOCUS       sequence-284760-        2657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2659
FEATURES             Location/Qualifiers
     source          1..2657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcagtg ggaaatgctg cagaatcttg acagcccctt tcaggatcag
       61 ctgcaccagc tttactcgca cagcctcctg cctgtggaca ttcgacagta cttggctgtc
      121 tggattgaag accagaactg gcaggaagct gcacttggga gtgatgattc caaggctacc
      181 atgctattct tccacttctt ggatcagctg aactatgagt gtggccgttg cagccaggac
      241 ccagagtcct tgttgctgca gcacaatttg cggaaattct gccgggacat tcagcccttt
      301 tcccaggatc ctacccagtt ggctgagatg atctttaacc tccttctgga agaaaaaaga
      361 attttgatcc aggctcagag ggcccaattg gaacaaggag agccagttct cgaaacacct
      421 gtggagagcc agcaacatga gattgaatcc cggatcctgg atttaagggc tatgatggag
      481 aagctggtaa aatccatcag ccaactgaaa gaccagcagg atgtcttctg cttccgatat
      541 aagatccagg ccaaagggaa gacaccctct ctggaccccc atcagaccaa agagcagaag
      601 attctgcagg aaactctcaa tgaactggac aaaaggagaa aggaggtgct ggatgcctcc
      661 aaagcactgc taggccgatt aactacccta atcgagctac tgctgccaaa gttggaggag
      721 tggaaggccc agcagcaaaa agcctgcatc agagctccca ttgaccacgg gttggaacag
      781 ctggagacat ggttcacagc tggagcaaag ctgttgtttc acctgaggca gctgctgaag
      841 gagctgaagg gactgagttg cctggttagc tatcaggatg accctctgac caaaggggtg
      901 gacctacgca acgcccaggt cacagagttg ctacagcgtc tgctccacag agcctttgtg
      961 gtagaaaccc agccctgcat gccccaaact ccccatcgac ccctcatcct caagactggc
     1021 agcaagttca ccgtccgaac aaggctgctg gtgagactcc aggaaggcaa tgagtcactg
     1081 actgtggaag tctccattga caggaatcct cctcaattac aaggcttccg gaagttcaac
     1141 attctgactt caaaccagaa aactttgacc cccgagaagg ggcagagtca gggtttgatt
     1201 tgggactttg gttacctgac tctggtggag caacgttcag gtggttcagg aaagggcagc
     1261 aataaggggc cactaggtgt gacagaggaa ctgcacatca tcagcttcac ggtcaaatat
     1321 acctaccagg gtctgaagca ggagctgaaa acggacaccc tccctgtggt gattatttcc
     1381 aacatgaacc agctctcaat tgcctgggct tcagttctct ggttcaattt gctcagccca
     1441 aaccttcaga accagcagtt cttctccaac ccccccaagg ccccctggag cttgctgggc
     1501 cctgctctca gttggcagtt ctcctcctat gttggccgag gcctcaactc agaccagctg
     1561 agcatgctga gaaacaagct gttcgggcag aactgtagga ctgaggatcc attattgtcc
     1621 tgggctgact tcactaagcg agagagccct cctggcaagt taccattctg gacatggctg
     1681 gacaaaattc tggagttggt acatgaccac ctgaaggatc tctggaatga tggacgcatc
     1741 atgggctttg tgagtcggag ccaggagcgc cggctgctga agaagaccat gtctggcacc
     1801 tttctactgc gcttcagtga atcgtcagaa gggggcatta cctgctcctg ggtggagcac
     1861 caggatgatg acaaggtgct catctactct gtgcaaccgt acacgaagga ggtgctgcag
     1921 tcactcccgc tgactgaaat catccgccat taccagttgc tcactgagga gaatatacct
     1981 gaaaacccac tgcgcttcct ctatccccga atcccccggg atgaagcttt tgggtgctac
     2041 taccaggaga aagttaatct ccaggaacgg aggaaatacc tgaaacacag gctcattgtg
     2101 gtctctaata gacaggtgga tgaactgcaa caaccgctgg agcttaagcc agagccagag
     2161 ctggagtcat tagagctgga actagggctg gtgccagagc cagagctcag cctggactta
     2221 gagccactgc tgaaggcagg gctggatctg gggccagagc tagagtctgt gctggagtcc
     2281 actctggagc ctgtgataga gcccacacta tgcatggtat cacaaacagt gccagagcca
     2341 gaccaaggac ctgtatcaca gccagtgcca gagccagatt tgccctgtga tctgagacat
     2401 ttgaacactg agccaatgga aatcttcaga aactgtgtaa agattgaaga aatcatgccg
     2461 aatggtgacc cactgttggc tggccagaac accgtggatg aggtttacgt ctcccgcccc
     2521 agccacttct acactgatgg acccttgatg ccttctgact tctagtaatg aactagtccg
     2581 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatagccgt
     2641 cgacgttgta agatgta
//
