LOCUS       sequence-284856-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2843
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcctcc ggaccagtgt agccagtata tgaccagctt gctcagccct
       61 gcagtcgacg acgagaaaga actacaggat atgaatgcta tggtgctgtc gcttactgaa
      121 gaggtcaaag aggaggaaga ggatgcacag cctgagcctg agcaaggcac agcagcagga
      181 gaaaagttaa agtcggcagg agcccaaggc ggagaagaaa aagatggcgg cggagaagaa
      241 aaagatggcg gcggcgccgg agttcctggc cacctatggg aaggaaacct cgagggcacc
      301 agcggcagcg atggcaacgt tgaggacagc gaccagagcg agaaggaacc tgggcagcag
      361 tattcgcgcc cacagggcgc cgtcgggggg ctggagcctg gcaacgcgca gcagcccaac
      421 gtccacgcct tcaccccatt gcagctgcag gagctggagt gcattttcca acgcgagcag
      481 ttccccagtg agttcctgcg aaggaggctg gcaagaagca tgaatgtgac tgaactcgca
      541 gtgcagattt ggtttgagaa tagaagagcc aaatggagga gacatcagag ggcattaatg
      601 gcaagaaaca tgctgccctt catggcagtg ggccagcctg tcatggtaac cgcagctgag
      661 gccataacgg cacccttgtt catcagcggg atgagagatg attacttctg ggaccacagc
      721 cattccagca gcctgtgttt ccccatgcca ccctttcctc ctccgtcctt gccccttcca
      781 ctcatgcttc ttccacctat gccacccgct ggccaggctg aatttggccc attccctttt
      841 gttatcgtgc cttctttcac attccccaat gtctagtaat gaactagtcc gtaacttgaa
      901 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagcctga catgggcgca
      961 ccgagaat
//
