LOCUS       sequence-284937-        2837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1164
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagcttc ctggcgccag gtggccggtg gccgaggccg atcccgggga
       61 cgggccactg ccgccccctc aggaaatgga gtccatctcc gcggcgccgg aggagggcga
      121 gagaaggggt cggtgggcgc agttccttct ggcaccagtc ccggaggagt cgcgaccacg
      181 gcggctgcag ggagcaggca cagccccgca ggatcccaag ccctgcagac taccgcagcc
      241 agcgagctaa tgtctcagaa aaaatttgaa gaaatcaaga aagctaacca agctgcagcc
      301 agaaaacttg ttgaagaaca gtttagctct tcatctgaag aaggagatga agattttgaa
      361 ggaaaacagg gaaaaatact tgcaaatacg tttataacat acactactca gacagatgga
      421 gatacacgtg aattagagcg aacaaaacaa tatgtaaatg aagcttttca agcaggggct
      481 atgacatgcc taatttgtat tgcttcggtg aagagaaacc aagcagtttg gagctgttcg
      541 ggatgtttct gtatatttca catgccctgt atccagaagt gggctaaaga cagccagttt
      601 cttgtatctt ctgtgactga tgatgatttt ggaaagaaag attgtccctg gccttgtcca
      661 aaatgtaggt ttgaatacaa acgatctgaa acacctagta ggtactattg ctattgtgga
      721 aaagtagaag atccaccttt agatccgtgg cttgtgcctc attcatgtgg ccaagtatgt
      781 gagcgtgaat ttaaacctcc ttgtggccat aaatgtttac tcctctgtca tccaggtccc
      841 tgccctcctt gtccaaagat ggtcacaact acttgttact gtaagaaagc aaaacctatc
      901 cctcgtaggt gcagtgccaa ggaatggtct tgtcagctgc catgtggaca gaagttgctt
      961 tgtgggcaac ataagtgtga aaatccttgt catgcaggaa gctgtcagcc ttgtccaaga
     1021 gttagtagac aaaagtgtgt ctgtggcaaa aaagtagctg aaagaagttg tgcaagtcca
     1081 ctatggcact gtgatcaagt atgtggaaaa acactgccat gtggtaatca cacatgtgag
     1141 caagtttgcc atgttggtgc ttgtggagaa tgtcctcgat ctgggaaaag gttctgtcca
     1201 tgtcagaaat caaagttttc tttgccttgt acagaagatg taccaacttg tggagacagt
     1261 tgtgacaaag tacttgaatg cggaatccat agatgttcac agcgttgtca ccgaggtccc
     1321 tgtgaaacat gtagacaaga agtggaaaag cattgtcgct gtggaaagca tacaaaacga
     1381 atgccttgtc ataaacctta tctgtgtgaa actaagtgtg ttaagatgcg tgactgtcag
     1441 aagcatcaat gtagaagaaa gtgttgccct ggaaactgtc caccttgtga tcaaaactgt
     1501 ggacggactt taggatgtag aaaccataag tgtccatctg tctgtcacag aggcagttgc
     1561 tatccctgcc cagaaaccgt agatgtgaag tgtaattgtg gcaatacaaa ggtgacagtg
     1621 ccctgtggcc gagaacgtac cacaagacca cccaagtgca aggagcaatg cagtcgacca
     1681 ccaacttgtc atcatacaag tcaagaaaaa catcgctgtc actttggttc ttgtccacca
     1741 tgtcatcaac cttgccaaaa agttttggag aaatgtggtc acttgtgtcc tgctccgtgt
     1801 catgatcaag cattaataaa gcagactggc aggcaccagc ctacaggccc ttgggaacag
     1861 ccttctgagc cagcatttat tcagactgca ttaccgtgtc ctccatgtca agttcctatt
     1921 cctatggaat gtcttgggaa acatgaggtg agtccactac catgccatgc tgtaggaccc
     1981 tactcttgta aaagagtttg tggaagaatc ttggattgtc agaatcacac atgtatgaaa
     2041 gaatgccaca aagtaaccaa aactgatggc tgcactggaa aaaacaaggc tggcccagaa
     2101 tgccttcatt gtgaggaagg gtgctccaag tcacggccac taggttgtct tcacccatgt
     2161 attttgcgat gtcaccctgg agaatgtcca ccttgtgttc agatgcttag aataaaatgt
     2221 cactgtaaga tcacaagcct gtatgtggaa tgtagaaaaa taaccacagc tgatgtaaat
     2281 gaaaagaacc tcctcagttg ttgcaaaaat cagtgcccta aagagcttcc ttgtggtcat
     2341 agatgcaaag agatgtgtca tcctggtgaa tgtcccttta actgcaacca gaaggtaaaa
     2401 cttagatgtc cttgtaaaag aataaaaaag gaattgcagt gcaacaaagt acgtgaaaat
     2461 caggtttcaa tagaatgtga cacaacgtgc aaggaaatga agcggaaagc atctgagata
     2521 aaagaagcag aagccaaagc tgctcttgaa gaagaaaaac gaagacaaca ggctgaacta
     2581 gaagcttttg aaaacagact gaagggtcgt cggaagaaga acaggaaaag agatgaagtg
     2641 gcagttgagc tatcactatg gcaaaaacat aaatattatc tcatttcagt gtgtggagtt
     2701 gtggttgtag tgtttgcctg gtacatcacc catgatgtca attagtaatg aactagtccg
     2761 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatgaccga
     2821 cgactgttca gatctat
//
