LOCUS       sequence-284958-        4037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1234
FEATURES             Location/Qualifiers
     source          1..4037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatctatt cgactttttc agagactggg acttggagca gcagtgtcac
       61 tatgaacaag accgtagtgc acttaaaaaa agggaatggg agcggaggaa tcaagaagtc
      121 cagcaagaag acgatctctt ttcttcaggc tttgatcttt ttggggagcc atacaaggta
      181 gctgaatata caaacaaagg tgatgcactt gccaaccgag tccagaacac gcttggaaac
      241 tatgatgaaa tgaagaattt gctaactaac cattctaatc agaatcacct agtgggaatt
      301 ccaaagaatt ctgtgcccca gaatcccaac aacaaaaatg aaccaagctt ttttccagaa
      361 caaaagaaca gaataattcc acctcaccag gataataccc atccttcagc accaatgcct
      421 ccaccttctg ttgtgatact gaattcaact ctaatacaca gcaacagaaa atcaaaacct
      481 gagtggtcac gtgatagtca taaccctagc actgtactgg caagccaggc cagtggtcag
      541 ccaaacaaga tgcagacttt gacacaggac cagtctcaag ccaaactgga agacttcttt
      601 gtctacccag ctgaacagcc ccagattgga gaagttgaag agtcaaaccc atctgcaaag
      661 gaagacagta accctaattc tagtggagaa gatgctttca aagaaatctt tcaatccaat
      721 tcaccggaag aatctgaatt cgccgtgcaa gcgcctgggt ctcccctagt ggcttcctct
      781 ttattagctc ctagcagtgg cctttcagtt caaaacttcc caccagggct ttactgcaaa
      841 acaagcatgg ggcagcaaaa gccaactgca tacgtcagac ccatggatgg ccaggaccag
      901 gcaccggaca tctcaccaac actgaaacct tcaattgaat ttgagaacag ctttgggaat
      961 ctgtcatttg gaacactctt ggatggaaaa cccagtgcag ccagttcaaa gactaaactg
     1021 ccaaagttca ccatcctcca aacaagtgaa gtaagccttc ccagtgatcc aagctgtgtt
     1081 gaagaaatct tgcgggagat gacccattcc tggcctactc ctctcacttc catgcatact
     1141 gctggacact ctgagcagag caccttttcc atcccaggac aggaatcgca gcatctgacc
     1201 ccaggattca ccttacaaaa gtggaatgac ccaaccacca gagcttctac aaagtcagtg
     1261 tctttcaaat cgatgcttga ggatgacctg aagctgagca gtgatgaaga tgaccttgag
     1321 cctgtgaaga ccttgaccac tcagtgcact gccactgagc tctaccaggc tgttgaaaag
     1381 gcaaaaccta ggaataatcc tgtgaaccca cccttggcca ctccccagcc cccacctgca
     1441 gtgcaagcca gcgggggttc tggcagctcc agcgaatcgg agagcagctc tgagtcggat
     1501 tcagacactg aaagtagcac cactgacagc gaatctaatg aggcacctcg tgtggcaact
     1561 ccagagcctg agccaccctc aaccaacaag tggcaactgg ataaatggct taacaaagtg
     1621 acatcccaga acaagtcttt tatttgtggc caaaatgaaa cacccatgga gactatttct
     1681 ctgcctcctc caatcatcca accaatggaa gtccagatga aagtgaagac gaatgccagt
     1741 caggtcccag ctgaacccaa agaaaggcct ctcctcagtc tcattaggga gaaagcccgt
     1801 ccacggccca ctcagaaaat tccagaaaca aaggctttga agcataagtt gtcaacaact
     1861 agtgagacag tgtctcaaag gacaattggg aaaaaacagc ccaaaaaagt tgagaagaac
     1921 accagcactg acgagtttac ctggcccaaa ccaaatatta ccagcagcac tcccaaagaa
     1981 aaagaaagtg tggagcttca tgacccacca agaggccgca acaaagccac tgcccacaaa
     2041 ccagccccta ggaaagaacc aagacctaac atccctttgg ctcccgagaa gaagaagtac
     2101 agagggcctg gcaagattgt gccaaagtct cgggaattca ttgaaacaga ttcatctaca
     2161 tctgactcca acacagatca ggaagagacc ctgcaaatca aagtcctgcc tccgtgcatt
     2221 atttctggag gtaatactgc caaatccaag gaaatctgtg gtgccagcct gaccctcagc
     2281 accttaatga gtagcagtgg cagcaacaac aacttatcca tcagtaatga agagccaaca
     2341 ttttcaccta ttcctgtcat gcaaactgaa atcctgtccc ctctgcgaga tcatgagaac
     2401 ctgaaaaacc tctgggtgaa gattgacctt gacttactct ctagagtacc tggccacagc
     2461 tcactccatg cagcacctgc caagccagac cacaaggaga ctgccacaaa acccaagcgt
     2521 cagacagctg tcacagctgt ggagaaacca gcccctaagg gcaaacgtaa gcacaagcca
     2581 atagaagttg cagagaagat ccctgagaag aagcagcgcc tggaggaggc cacaactatc
     2641 tgcttgctcc ctccttgcat ctcaccagcc ccaccccaca agcctcccaa cactagagaa
     2701 aataattcat ccaggagagc aaatagaaga aaggaagaaa aactatttcc tcctccactt
     2761 tccccactgc cagaggaccc tccacgccgc agaaatgtca gtggcaataa tggtcccttt
     2821 ggtcaagaca aaaacatcgc catgactgga caaatcacat ctaccaaacc taagagaact
     2881 gaaggcaaat tctgtgctac tttcaaaggg atatcggtaa atgagggaga cactccaaaa
     2941 aaggcatcct ctgccaccat cactgtcacc aatactgcta ttgccactgc tactgtcact
     3001 gctactgcca ttgtcaccac cactgtcaca gctactgcca ccgccacggc caccaccaca
     3061 actactacca ctaccatttc caccatcacc tctaccatca ctactggcct catggatagc
     3121 agtcacctgg agatgacgtc ctgggcggct ctgccccttc tatccagcag cagcactaat
     3181 gtccggagac ccaagctcac ttttgatgac tcggttcaca atgctgatta ttacatgcaa
     3241 gaagctaaga agctgaagca caaagctgat gcactgttcg agaaatttgg caaagctgtg
     3301 aattatgctg atgccgccct ctccttcact gaatgtggca atgccatgga acgcgaccct
     3361 ctggaagcaa agtccccata caccatgtac tctgagactg tggagctcct caggtatgca
     3421 atgaggctga agaactttgc aagtcccttg gcttcggatg gggacaaaaa gctagcagta
     3481 ctatgctacc gatgtttatc actcctctat ttgagaatgt ttaagctgaa gaaggaccat
     3541 gctatgaagt actccagatc actgatggaa tattttaagc aaaatgcttc aaaagtcgca
     3601 cagataccct ctccatgggt aagcaatgga aagaacactc catccccagt gtctctcaac
     3661 aacgtctccc ccatcaacgc aatggggaac tgtaacaatg gcccagtcac cattccccag
     3721 cgcattcacc acatggctgc cagccacgtc aacatcacta gcaatgtgtt acggggctat
     3781 gaacactggg atatggccga caaactgaca agagaaaaca aagaattctt tggtgatctg
     3841 gacacgctga tggggcctct gacccagcac agcagcatga ccaatcttgt ccgctacgtt
     3901 cgccaaggac tgtgttggct gcgcatcgat gcccacttgt tgtagtaatg aactagtccg
     3961 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatggctgt
     4021 cataagggaa caggtag
//
