LOCUS       sequence-284999-       16885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1618
FEATURES             Location/Qualifiers
     source          1..16885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12742..13330
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12795..12815)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13213..13224)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13402..13635
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13713..13834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13750..13769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13804..13823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13874..14009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14029..14048)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14030..14048)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14058..14075)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14058..14074)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14067..14089)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14216..14671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14303..14322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14513..14534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14697..14801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14802..14870,14871..15662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15020..15039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15833..16421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16322..16341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16575..16592
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16709..16730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16745..16750,16751..16768,16769..16775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16783..16799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16788..16810
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16807..16823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16807..16823
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16842..16862
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16844..16862
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gcgcacaggc cggtggaatg
     4141 ggtccaggcc gtggtcagcc gcttcgacga gcagcttcca ataaaaacag gacagcagaa
     4201 cacacatacc aaagtcagta ctgagcacaa caaggaatgt ctaatcaata tttccaaata
     4261 caagttttct ttggttataa gcggcctcac tactatttta aagaatgtta acaatatgag
     4321 aatatttgga gaagctgctg aaaaaaattt atatctctct cagttgatta tattggatac
     4381 actggaaaaa tgtcttgctg ggcaaccaaa ggacacaatg agattagatg aaacgatgct
     4441 ggtcaaacag ttgctgccag aaatctgcca ttttcttcac acctgtcgtg aaggaaacca
     4501 gcatgcagct gaacttcgga attctgcctc tggggtttta ttttctctca gctgcaacaa
     4561 cttcaatgca gtctttagtc gcatttctac caggttacag gaattaactg tttgttcaga
     4621 agacaatgtt gatgttcatg atatagaatt gttacagtat atcaatgtgg attgtgcaaa
     4681 attaaaacga ctcctgaagg aaacagcatt taaatttaaa gccctaaaga aggttgcgca
     4741 gttagcagtt ataaatagcc tggaaaaggc attttggaac tgggtagaaa attatccaga
     4801 tgaatttaca aaactgtacc agatcccaca gactgatatg gctgaatgtg cagaaaagct
     4861 atttgacttg gtggatggtt ttgctgaaag caccaaacgt aaagcagcag tttggccact
     4921 acaaatcatt ctccttatct tgtgtccaga aataatccag gatatatcca aagacgtggt
     4981 tgatgaaaac aacatgaata agaagttatt tctggacagt ctacgaaaag ctcttgctgg
     5041 ccatggagga agtaggcagc tgacagaaag tgctgcaatt gcctgtgtca aactgtgtaa
     5101 agcaagtact tacatcaatt gggaagataa ctctgtcatt ttcctacttg ttcagtccat
     5161 ggtggttgat cttaagaacc tgctttttaa tccaagtaag ccattctcaa gaggcagtca
     5221 gcctgcagat gtggatctaa tgattgactg ccttgtttct tgctttcgta taagccctca
     5281 caacaaccaa cactttaaga tctgcctggc tcagaattca ccttctacat ttcactatgt
     5341 gctggtaaat tcactccatc gaatcatcac caattccgca ttggattggt ggcctaagat
     5401 tgatgctgtg tattgtcact cggttgaact tcgaaatatg tttggtgaaa cacttcataa
     5461 agcagtgcaa ggttgtggag cacacccagc aatacgaatg gcaccgagtc ttacatttaa
     5521 agaaaaagta acaagcctta aatttaaaga aaaacctaca gacctggaga caagaagcta
     5581 taagtatctt ctcttgtcca tggtgaaact aattcatgca gatccaaagc tcttgctttg
     5641 taatccaaga aaacaggggc ccgaaaccca aggcagtaca gcagaattaa ttacagggct
     5701 cgtccaactg gtccctcagt cacacatgcc agagattgct caggaagcaa tggaggctct
     5761 gctggttctt catcagttag atagcattga tttgtggaat cctgatgctc ctgtagaaac
     5821 attttgggag attagctcac aaatgctttt ttacatctgc aagaaattaa ctagtcatca
     5881 aatgcttagt agcacagaaa ttctcaagtg gttgcgggaa atattgatct gcaggaataa
     5941 atttcttctt aaaaataagc aggcagatag aagttcctgt cactttctcc ttttttacgg
     6001 ggtaggatgt gatattcctt ctagtggaaa taccagtcaa atgtccatgg atcatgaaga
     6061 attactacgt actcctggag cctctctccg gaagggaaaa gggaactcct ctatggatag
     6121 tgcagcagga tgcagcggaa cccccccgat ttgccgacaa gcccagacca aactagaagt
     6181 ggccctgtac atgtttctgt ggaaccctga cactgaagct gttctggttg ccatgtcctg
     6241 tttccgccac ctctgtgagg aagcagatat ccggtgtggg gtggatgaag tgtcagtgca
     6301 taacctcttg cccaactata acacattcat ggagtttgcc tctgtcagca atatgatgtc
     6361 aacaggaaga gcagcacttc agaaaagagt gatggcactg ctgaggcgca ttgagcatcc
     6421 cactgcagga aacactgagg cttgggaaga tacacatgca aaatgggaac aagcaacaaa
     6481 gctaatcctt aactatccaa aagccaaaat ggaagatggc caggctgctg aaagccttca
     6541 caagaccatt gttaagaggc gaatgtccca tgtgagtgga ggaggatcca tagatttgtc
     6601 tgacacagac tccctacagg aatggatcaa catgactggc ttcctttgtg cccttggggg
     6661 agtgtgcctc cagcagagaa gcaattctgg cctggcaacc tatagcccac ccatgggtcc
     6721 agtcagtgaa cgtaagggtt ctatgatttc agtgatgtct tcagagggaa acgcagatac
     6781 acctgtcagc aaatttatgg atcggctgtt gtccttaatg gtgtgtaacc atgagaaagt
     6841 gggacttcaa atacggacca atgttaagga tctggtgggt ctagaattga gtcctgctct
     6901 gtatccaatg ctatttaaca aattgaagaa taccatcagc aagttttttg actcccaagg
     6961 acaggtttta ttgactgata ccaatactca atttgtagaa caaaccatag ctataatgaa
     7021 gaacttgcta gataatcata ctgaaggcag ctctgaacat ctagggcaag ctagcattga
     7081 aacaatgatg ttaaatctgg tcaggtatgt tcgtgtgctt gggaatatgg tccatgcaat
     7141 tcaaataaaa acgaaactgt gtcaattagt tgaagtaatg atggcaagga gagatgacct
     7201 ctcattttgc caagagatga aatttaggaa taagatggta gaatacctga cagactgggt
     7261 tatgggaaca tcaaaccaag cagcagatga tgatgtaaaa tgtcttacaa gagatttgga
     7321 ccaggcaagc atggaagcag tagtttcact tctagctggt ctccctctgc agcctgaaga
     7381 aggagatggt gtggaattga tggaagccaa atcacagtta tttcttaaat acttcacatt
     7441 atttatgaac cttttgaatg actgcagtga agttgaagat gaaagtgcgc aaacaggtgg
     7501 caggaaacgt ggcatgtctc ggaggctggc atcactgagg cactgtacgg tccttgcaat
     7561 gtcaaactta ctcaatgcca acgtagacag tggtctcatg cactccatag gcttaggtta
     7621 ccacaaggat ctccagacaa gagctacatt tatggaagtt ctgacaaaaa tccttcaaca
     7681 aggcacagaa tttgacacac ttgcagaaac agtattggct gatcggtttg agagattggt
     7741 ggaactggtc acaatgatgg gtgatcaagg agaactccct atagcgatgg ctctggccaa
     7801 tgtggttcct tgttctcagt gggatgaact agctcgagtt ctggttactc tgtttgattc
     7861 tcggcattta ctctaccaac tgctctggaa catgttttct aaagaagtag aattggcaga
     7921 ctccatgcag actctcttcc gaggcaacag cttggccagt aaaataatga cattctgttt
     7981 caaggtatat ggtgctacct atctacaaaa actcctggat cctttattac gaattgtgat
     8041 cacatcctct gattggcaac atgttagctt tgaagtggat cctaccaggt tagaaccatc
     8101 agagagcctt gaggaaaacc agcggaacct ccttcagatg actgaaaagt tcttccatgc
     8161 catcatcagt tcctcctcag aattcccccc tcaacttcga agtgtgtgcc actgtttata
     8221 ccaggcaact tgccactccc tactgaataa agctacagta aaagaaaaaa aggaaaacaa
     8281 aaaatcagtg gttagccagc gtttccctca gaacagcatc ggtgcagtag gaagtgccat
     8341 gttcctcaga tttatcaatc ctgccattgt ctcaccgtat gaagcaggga ttttagataa
     8401 aaagccacca cctagaatcg aaaggggctt gaagttaatg tcaaagatac ttcagagtat
     8461 tgccaatcat gttctcttca caaaagaaga acatatgcgg cctttcaatg attttgtgaa
     8521 aagcaacttt gatgcagcac gcaggttttt ccttgatata gcatctgatt gtcctacaag
     8581 tgatgcagta aatcatagtc tttccttcat aagtgacggc aatgtgcttg ctttacatcg
     8641 tctactctgg aacaatcagg agaaaattgg gcagtatctt tccagcaaca gggatcataa
     8701 agctgttgga agacgacctt ttgataagat ggcaacactt cttgcatacc tgggtcctcc
     8761 agagcacaaa cctgtggcag atacacactg gtccagcctt aaccttacca gttcaaagtt
     8821 tgaggaattt atgactaggc atcaggtaca tgaaaaagaa gaattcaagg ctttgaaaac
     8881 gttaagtatt ttctaccaag ctgggacttc caaagctggg aatcctattt tttattatgt
     8941 tgcacggagg ttcaaaactg gtcaaatcaa tggtgatttg ctgatatacc atgtcttact
     9001 gactttaaag ccatattatg caaagccata tgaaattgta gtggacctta cccataccgg
     9061 gcctagcaat cgctttaaaa cagactttct ctctaagtgg tttgttgttt ttcctggctt
     9121 tgcttacgac aacgtctccg cagtctatat ctataactgt aactcctggg tcagggagta
     9181 caccaagtat catgagcggc tgctgactgg cctcaaaggt agcaaaaggc ttgttttcat
     9241 agactgtcct gggaaactgg ctgagcacat agagcatgaa caacagaaac tacctgctgc
     9301 caccttggct ttagaagagg acctgaaggt attccacaat gctctcaagc tagctcacaa
     9361 agacaccaaa gtttctatta aagttggttc tactgctgtc caagtaactt cagcagagcg
     9421 aacaaaagtc ctagggcaat cagtctttct aaatgacatt tattatgctt cggaaattga
     9481 agaaatctgc ctagtagatg agaaccagtt caccttaacc attgcaaacc agggcacgcc
     9541 gctcaccttc atgcaccagg agtgtgaagc cattgtccag tctatcattc atatccggac
     9601 ccgctgggaa ctgtcacagc ccgactctat cccccaacac accaagattc ggccaaaaga
     9661 tgtccctggg acactgctca atatcgcatt acttaattta ggcagttctg acccgagttt
     9721 acggtcagct gcctataatc ttctgtgtgc cttaacttgt acctttaatt taaaaatcga
     9781 gggccagtta ctagagacat caggtttatg tatccctgcc aacaacaccc tctttattgt
     9841 ctctattagt aagacactgg cagccaatga gccacacctc acgttagaat ttttggaaga
     9901 gtgtatttct ggatttagca aatctagtat tgaattgaaa cacctttgtt tggaatacat
     9961 gactccatgg ctgtcaaatc tagttcgttt ttgcaagcat aatgatgatg ccaaacgaca
    10021 aagagttact gctattcttg acaagctgat aacaatgacc atcaatgaaa aacagatgta
    10081 cccatctatt caagcaaaaa tatggggaag ccttgggcag attacagatc tgcttgatgt
    10141 tgtactagac agtttcatca aaaccagtgc aacaggtggc ttgggatcaa taaaagctga
    10201 ggtgatggca gatactgctg tagctttggc ttctggaaat gtgaaattgg tttcaagcaa
    10261 ggttattgga aggatgtgca aaataattga caagacatgc ttatctccaa ctcctacttt
    10321 agaacaacat cttatgtggg atgatattgc tattttagca cgctacatgc tgatgctgtc
    10381 cttcaacaat tcccttgatg tggcagctca tcttccctac ctcttccacg ttgttacttt
    10441 cttagtagcc acaggtccgc tctcccttag agcttccaca catggactgg tcattaatat
    10501 cattcactct ctgtgtactt gttcacagct tcattttagt gaagagacca agcaagtttt
    10561 gagactcagt ctgacagagt tctcattacc caaattttac ttgctgtttg gcattagcaa
    10621 agtcaagtca gctgctgtca ttgccttccg ttccagttac cgggacaggt cattctctcc
    10681 tggctcctat gagagagaga cttttgcttt gacatccttg gaaacagtca cagaagcttt
    10741 gttggagatc atggaggcat gcatgagaga tattccaacg tgcaagtggc tggaccagtg
    10801 gacagaacta gctcaaagat ttgcattcca atataatcca tccctgcaac caagagctct
    10861 tgttgtcttt gggtgtatta gcaaacgagt gtctcatggg cagataaagc agataatccg
    10921 tattcttagc aaggcacttg agagttgctt aaaaggacct gacacttaca acagtcaagt
    10981 tctgatagaa gctacagtaa tagcactaac caaattacag ccacttctta ataaggactc
    11041 gcctctgcac aaagccctct tttgggtagc tgtggctgtg ctgcagcttg atgaggtcaa
    11101 cttgtattca gcaggtaccg cacttcttga acaaaacctg catactttag atagtctccg
    11161 tatattcaat gacaagagtc cagaggaagt atttatggca atccggaatc ctctggagtg
    11221 gcactgcaag caaatggatc attttgttgg actcaatttc aactctaact ttaactttgc
    11281 attggttgga caccttttaa aagggtacag gcatccttca cctgctattg ttgcaagaac
    11341 agtcagaatt ttacatacac tactaactct ggttaacaaa cacagaaatt gtgacaaatt
    11401 tgaagtgaat acacagagcg tggcctactt agcagcttta cttacagtgt ctgaagaagt
    11461 tcgaagtcgc tgcagcctaa aacatagaaa gtcacttctt cttactgata tttcaatgga
    11521 aaatgttcct atggatacat atcccattca tcatggtgac ccttcctata ggacactaaa
    11581 ggagactcag ccatggtcct ctcccaaagg ttctgaagga taccttgcag ccacctatcc
    11641 aactgtcggc cagaccagtc cccgagccag gaaatccatg agcctggaca tggggcaacc
    11701 ttctcaggcc aacactaaga agttgcttgg aacaaggaaa agttttgatc acttgatatc
    11761 agacacaaag gctcctaaaa ggcaagaaat ggaatcaggg atcacaacac cccccaaaat
    11821 gaggagagta gcagaaactg attatgaaat ggaaactcag aggatttcct catcacaaca
    11881 gcacccacat ttacgtaaag tttcagtgtc tgaatcaaat gttctcttgg atgaagaagt
    11941 acttactgat ccgaagatcc aggcgctgct tcttactgtt ctagctacac tggtaaaata
    12001 taccacagat gagtttgatc aacgaattct ttatgaatac ttagcagagg ccagtgttgt
    12061 gtttcccaaa gtctttcctg ttgtgcataa tttgttggac tctaagatca acaccctgtt
    12121 atcattgtgc caagatccaa atttgttaaa tccaatccat ggaattgtgc agagtgtggt
    12181 gtaccatgaa gaatccccac cacaatacca aacatcttac ctgcaaagtt ttggttttaa
    12241 tggcttgtgg cggtttgcag gaccgttttc aaagcaaaca caaattccag actatgctga
    12301 gcttattgtt aagtttcttg atgccttgat tgacacgtac ctgcctggaa ttgatgaaga
    12361 aaccagtgaa gaatccctcc tgactcccac atctccttac cctcctgcac tgcagagcca
    12421 gcttagtatc actgccaacc ttaacctttc taattccatg acctcacttg caacttccca
    12481 gcattcccca ggaatcgaca aggagaacgt tgaactctcc cctaccactg gccactgtaa
    12541 cagtggacga actcgccacg gatccgcaag ccaagtgcag aagcaaagaa gcgctggcag
    12601 tttcaaacgt aatagcatta agaagatcgt gtagtaatga actagtccgt aacttgaaag
    12661 tatttcgatt tcttggcttt atatatcttg tggaaaggac gacgcaacat tgagggtagt
    12721 ccatccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
    12781 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    12841 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    12901 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    12961 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    13021 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    13081 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    13141 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    13201 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    13261 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    13321 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
    13381 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    13441 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    13501 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    13561 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    13621 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
    13681 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
    13741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    13801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    13861 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    13921 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    13981 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    14041 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    14101 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    14161 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    14221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    14281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    14341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    14401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    14461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    14521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    14581 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    14641 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    14701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    14761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    14821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    14881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    14941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    15001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    15061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    15121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    15181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    15241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    15301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    15361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    15421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    15481 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    15541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    15601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    15661 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    15721 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    15781 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    15841 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    15901 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    15961 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    16021 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    16081 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    16141 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    16201 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    16261 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    16321 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    16381 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    16441 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    16501 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    16561 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    16621 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    16681 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    16741 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    16801 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    16861 gggaacaaaa gctggagctg caagc
//
