LOCUS       sequence-285060-        8006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1892
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6221..6230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgtctggcgg cgccgcagag aagcagagca gcactcccgg ttccctgttc
       61 ctctcgccgc cggctcctgc ccccaagaat ggctccagct ccgattcctc cgtgggggag
      121 aaactgggag ccgcggccgc cgacgctgtg accggcagga ccgaggagta caggcgccgc
      181 cgccacacta tggacaagga cagccgtggg gcggccgcga ccactaccac cactgagcac
      241 cgcttcttcc gccggagcgt catctgtgac tccaatgcca ctgcactgga gcttcccggc
      301 cttcctcttt ccctgcccca gcccagcatc cccgcggctg tcccgcagag tgctccaccg
      361 gagccccacc gggaagagac cgtgaccgcc accgccactt cccaggtagc ccagcagcct
      421 ccagccgctg ccgcccctgg ggaacaggcc gtcgcgggcc ctgccccctc gactgtcccc
      481 agcagtacca gcaaagaccg cccagtgtcc cagcctagcc ttgtggggag caaagaggag
      541 ccgccgccgg cgagaagtgg cagcggcggc ggcagcgcca aggagccaca ggaggaacgg
      601 agccagcagc aggatgatat cgaagagctg gagaccaagg ccgtgggaat gtctaacgat
      661 ggccgctttc tcaagtttga catcgaaatc ggcagaggct cctttaagac ggtctacaaa
      721 ggtctggaca ctgaaaccac cgtggaagtc gcctggtgtg aactgcagga tcgaaaatta
      781 acaaagtctg agaggcagag atttaaagaa gaagctgaaa tgttaaaagg tcttcagcat
      841 cccaatattg ttagatttta tgattcctgg gaatccacag taaaaggaaa gaagtgcatt
      901 gttttggtga ctgaacttat gacgtctgga acacttaaaa cgtatctgaa aaggtttaaa
      961 gtgatgaaga tcaaagttct aagaagctgg tgccgtcaga tccttaaagg tcttcagttt
     1021 cttcatactc gaactccacc tatcattcac cgcgatctta aatgtgacaa catctttatc
     1081 accggcccta ctggctcagt caagattgga gacctcggtc tggcaaccct gaagcgggct
     1141 tcttttgcca agagtgtgat aggtacccca gagttcatgg cccctgagat gtatgaggag
     1201 aaatatgatg aatccgttga cgtttatgct tttgggatgt gcatgcttga gatggctaca
     1261 tctgaatatc cttactcgga gtgccaaaat gctgcacaga tctaccgtcg cgtgaccagt
     1321 ggggtgaagc cagccagttt tgacaaagta gcaattcctg aagtgaagga aattattgaa
     1381 ggatgcatac gacaaaacaa agatgaaaga tattccatca aagacctttt gaaccatgcc
     1441 ttcttccaag aggaaacagg agtacgggta gaattagcag aagaagatga tggagaaaaa
     1501 atagccataa aattatggct acgtattgaa gatattaaga aattaaaggg aaaatacaaa
     1561 gataatgaag ctattgagtt ttcttttgat ttagagagag atgtcccaga agatgttgca
     1621 caagaaatgg tagagtctgg gtatgtctgt gaaggtgatc acaagaccat ggctaaagct
     1681 atcaaagaca gagtatcatt aattaagagg aaacgagagc agcggcagtt ggtacgggag
     1741 gagcaagaaa aaaaaaagca ggaagagagc agtctcaaac agcaggtaga acaatccagt
     1801 gcttcccaga caggaatcaa gcagctccct tctgctagca ccggcatacc tactgcttct
     1861 accacttcag cttcagtttc tacacaagta gaacctgaag aacctgaggc agatcaacat
     1921 caacaactac agtaccagca acccagtata tctgtgttat ctgatgggac ggttgacagt
     1981 ggtcagggat cctctgtctt cacagaatct cgagtgagca gccaacagac agtttcatat
     2041 ggttcccaac atgaacaggc acattctaca ggcacagtcc cagggcatat accttctact
     2101 gtccaagcac agtctcagcc ccatggggta tatccaccct caagtgtgcc tcgtcgtggc
     2161 cgtagcatgt cggtttgtgt tcccatcttt ctgctgttgc ctctgtgtcc cgcatctctc
     2221 ccagtgctct tccaccccac cgccagtact gtctgcacct ctttctcctt ccctcctccg
     2281 gactgccccg aggaaacttt tgccgaaaag ctttctaaag cattggagag tgtcctgcct
     2341 atgcactctg cctctcagcg caagcaccga cgctccagcc tgccttccct ctttgtcagt
     2401 actcctcagt ccatggcgca tccgtgtggg gggaccccaa catacccaga atcacagata
     2461 tttttcccaa ctattcatga acgtccagtt tctttttcac cacctcccac ctgcccaccg
     2521 aaagtagcca tttcccagcg gcgtaagagc acctccttcc tggaagccca aactcaccac
     2581 ttccaacccc tgctgaggac tgttggccaa agtcttcttc cacctggtgg cagcccaact
     2641 aactggacac cagaggccgt agttatgttg ggtactacag ccagtagagt aactggagag
     2701 tcatgtgaga tacaggtcca tcctatgttt gaaccatctc aagtttacag tgactataga
     2761 cctggactag tacttccaga agaagctcac tattttattc ctcaggaagc agtgtatgta
     2821 gctggggtac attaccaggc ccgggtggca gaacagtatg agggcattcc atacaactca
     2881 tcagtactgt caagtcctat gaaacagata cctgaacaga agccagtaca agggggccct
     2941 acttcaagtt ctgtctttga atttccatct ggacaggctt tcctggtagg acaccttcag
     3001 aatctaagat tagattctgg attgggtccg ggatctcccc tctctagtat ttctgcacct
     3061 atcagtacag atgctacacg tttgaaattt caccctgtct ttgttcctca ttctgcgcct
     3121 gctgtgttaa ctcataacaa tgagagcaga agcaactgtg tatttgaatt tcatgttcac
     3181 acaccaagct cctcttcagg agaaggaggt ggaattttac ctcagcgtgt ttaccgaaat
     3241 cggcaggttg cagtggactt gaatcaagaa gaactgcctc ctcaatcagt tggattacat
     3301 ggctacttgc agcctgtgac tgaagaaaag cataattacc atgccccaga attgaccgtt
     3361 tctgtggtag agcctatcgg acagaactgg ccaataggaa gcccagaata ttccagtgat
     3421 tcctcacaaa tcacttcttc agaccccagt gattttcagt cacctccccc tacaggggga
     3481 gcagctgcac cttttggctc tgacgtctca atgcccttta tccatctgcc tcagacagtg
     3541 ttacaagaat ccccactttt cttctgtttc ccccaaggaa ccacatctca gcaggtctta
     3601 actgcctcat tttcttcagg aggatctgca cttcatccac aggcacaggg gcagagccag
     3661 ggtcagccat cctcaagtag cttaacaggg gtttcatctt cccaacccat acaacatcct
     3721 cagcagcagc agggaataca gcagacagcc cctcctcaac agacagtgca gtattcactt
     3781 tcacagacat caacctccag tgaggccact actgcacagc cagtgagtca gcctcaagct
     3841 ccacaagtct tgcctcaagt atcagctgga aaacagagta ctcagggagt ctctcaggtt
     3901 gctcctgcag agccagttgc agtagcacag acccaagcta cccagccgac cactttggct
     3961 tcctctgtag acagtgcaca ttcagatgtt gcttcaggta tgagtgatgg caatgagaac
     4021 gtcccatctt ccagtggaag gcatgaagga agaactacaa aacggcatta ccgaaaatct
     4081 gtaaggagtc gctctcgaca tgaaaaaact tcacgcccaa aattaagaat tttgaatgtt
     4141 tcaaataaag gagaccgagt agtagaatgt caattagaga ctcataatag gaaaatggtt
     4201 acattcaaat ttgacctaga tggtgacaac cccgaggaga tagcaacaat tatggtgaac
     4261 aatgacttta ttctagcaat agagagagag tcgtttgtgg atcaagtgcg agaaattatt
     4321 gaaaaagctg atgaaatgct cagtgaggat gtcagtgtgg aaccagaggg tgatcaggga
     4381 ttggagagtc tacaaggaaa ggatgactat ggcttttcag gttctcagaa attggaagga
     4441 gagttcaaac aaccaattcc tgcgtcttcc atgccacagc aaataggcat tcctaccagt
     4501 tctttaactc aagttgttca ttctgcggga aggcggttta tagtgagtcc tgtgccagaa
     4561 agccgattac gagaatcaaa agttttcccc agtgaaataa cagatacagt tgctgcctct
     4621 acagctcaga gccctggaat gaacttgtct cactctgcat catcccttag tctacaacag
     4681 gccttttctg aacttagacg tgcccaaatg acagaaggac ccaacacagc acctccaaac
     4741 tttagtcata caggaccaac atttccagta gtacctcctt tcttaagtag cattgctgga
     4801 gtcccaacca cagcagcagc cacagcacca gtccctgcaa caagcagccc tcctaatgac
     4861 atttccacat cagtaattca gtctgaggtt acagtgccca ctgaagaggg gattgctgga
     4921 gttgccacca gcacaggtgt ggtaacttca ggtggtctcc ccataccacc tgtgtctgaa
     4981 tcaccagtac tttccagcgt agtttcaagt atcacaatac ctgcagttgt ctcaatatct
     5041 actacatccc cgtcacttca agtccccaca tccacatctg agatcgttgt ttctagtaca
     5101 gcactgtatc cttcagtaac agtttcagca acttcagcct ctgcaggggg cagtactgct
     5161 accccaggtc ctaagcctcc agctgtagta tctcagcagg cagcaggcag cactactgtg
     5221 ggagccacat taacatcagt ttctaccacc acttcattcc caagcacagc ttcacagctg
     5281 tgcattcagc ttagcagcag tacttctact cctactttag ctgaaaccgt ggtagttagc
     5341 gcacactcac tagataagac atctcatagc agtacaactg gattggcttt ctccctctct
     5401 gcaccatctt cctcttcctc tcctggagca ggagtgtcta gttatatttc tcagcctggt
     5461 gggctgcatc ctttggtcat tccatcagtg atagcttcta ctcctattct tccccaagca
     5521 gcaggaccta cttctacacc tttattaccc caagtaccta gtatcccacc cttggtacag
     5581 cctgttgcca atgtgcctgc tgtacagcag acactaattc atagtcagcc tcaaccagct
     5641 ttgcttccca accagcccca tactcattgt cctgaagtag attctgatac acaacccaaa
     5701 gctcctggaa ttgatgacat aaagactcta gaagaaaagc tgcggtctct gttcagtgaa
     5761 cacagctcat ctggagctca gcatgcctct gtctcactgg agacctcact agtcatagag
     5821 agcactgtca caccaggcat cccaactact gctgttgcac caagcaaact cctgacttct
     5881 accacaagta cttgcttacc accaaccaat ttaccactag gaacagttgc tttgccagtt
     5941 acaccagtgg tcacacctgg gcaagtttct accccagtca gcactactac atcaggagtg
     6001 aaacctggaa ctgctccctc caagccacct ctaactaagg ctccggtgct gccagtgggt
     6061 actgaacttc cagcaggtac tctacccagc gagcagctgc caccttttcc aggaccttct
     6121 ctaacccagt cccagcaacc tctagaggat cttgatgctc aattgagaag aacacttagt
     6181 ccagagatga tcacagtgac ttctgcggtt ggtcctgtgt ccatggcggc tccaacagca
     6241 atcacagaag caggaacaca gcctcagaag ggtgtttctc aagtcaaaga aggccctgtc
     6301 ctagcaacta gttcaggagc tggtgttttt aagatgggac gatttcaggt ttctgttgca
     6361 gcagacggtg cccagaaaga gggtaaaaat aagtcagaag atgcaaagtc tgttcatttt
     6421 gaatccagca cctcagagtc ctcagtgcta tcaagtagta gtccagagag taccttggtg
     6481 aaaccagagc cgaatggcat aaccatccct ggtatctctt cagatgtgcc agagagtgcc
     6541 cacaaaacta ctgcctcaga ggcaaagtca gacactgggc agcctaccaa ggttggacgt
     6601 tttcaggtga caactacagc aaacaaagtg ggtcgtttct ctgtatcaaa aactgaggac
     6661 aagatcactg acacaaagaa agaaggacca gtggcatctc ctccttttat ggatttggaa
     6721 caagctgttc ttcctgctgt gataccaaag aaagagaagc ctgaactgtc agagccttca
     6781 catctaaatg ggccgtcttc tgacccggag gccgcttttt taagtaggga tgtggatgat
     6841 ggttccggta gtccacactc gccccatcag ctgagctcaa agagccttcc tagccagaat
     6901 ctaagtcaaa gccttagtaa ttcatttaac tcctcttaca tgagtagcga caatgagtca
     6961 gatatcgaag atgaagactt aaagttagag ctgcgacgac tacgagataa acatctcaaa
     7021 gagattcagg acctgcagag tcgccagaag catgaaattg aatctttgta taccaaactg
     7081 ggcaaggtgc cccctgctgt tattattccc ccagctgctc ccctttcagg gagaagacga
     7141 cgacccacta aaagcaaagg cagcaaatct agtcgaagca gttccttggg gaataaaagc
     7201 ccccagcttt caggtaacct gtctggtcag agtgcagctt cagtcttgca cccccagcag
     7261 accctccacc ctcctggcaa catcccagag tccgggcaga atcagctgtt acagcccctt
     7321 aagccatctc cctccagtga caacctctat tcagccttca ccagtgatgg tgccatttca
     7381 gtaccaagcc tttctgctcc aggtcaagga accagcagca caaacactgt tggggcaaca
     7441 gtgaacagcc aagccgccca agctcagcct cctgccatga cgtccagcag gaagggcaca
     7501 ttcacagatg acttgcacaa gttggtagac aattgggccc gagatgccat gaatctctca
     7561 ggcaggagag gaagcaaagg gcacatgaat tacgagggcc ctggaatggc aaggaagttc
     7621 tctgcacctg ggcaactgtg catctccatg acctcgaacc tgggtggctc tgcccccatc
     7681 tctgcagcat cagctacctc tctaggtcac ttcaccaagt ctatgtgccc cccacagcag
     7741 tatggctttc cagctacccc atttggcgct caatggagtg ggacgggtgg cccagcacca
     7801 cagccacttg gccagttcca acctgtggga actgcctcct tgcagaattt caacatcagc
     7861 aatttgcaga aatccatcag caacccccca ggctccaacc tgcggaccac ttagtaatga
     7921 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     7981 gaggctgatg tcagggatgg tgtgac
//
