LOCUS       sequence-285102-        4511 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2083
FEATURES             Location/Qualifiers
     source          1..4511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtaga tgctgaaaga gagaagataa cacaggagat caaggagctg
       61 gaaaggattt tggatcccgg ctcctcgggc tcccacgtgg agatctcaga atcaagtctc
      121 gagtcagatt ctgaagcaga ttcactgcct tctgaggact tggatcctgc cgatcccccg
      181 atctcggaag aagaaaggtg gggcgaagcc agcaatgacg aggacgatcc caaggataaa
      241 accctccctg aagacccaga aacctgcctg cagctgaaca tggtctacca ggaggtcatc
      301 caggagaagc tggctgaggc caacctgctg ctggcccaga accgggagca gcaggaggaa
      361 ctcatgaggg atctggctgg gtccaaaggc accaaggtga aagatggcaa aagcctgccc
      421 ccaagcacat acatggggca cttcatgaag ccgtatttca aggacaaggt cacgggcgtg
      481 gggccacctg ccaacgagga cacacgagag aaggctgccc aggggatcaa ggctttcgag
      541 gagctccttg tgaccaaatg gaaaaactgg gaaaaggcct tgctccgaaa gtcagtggtg
      601 agtgaccgcc tgcagcgatt gcttcagccc aagttactga agctcgagta cttgcaccag
      661 aagcagagca aagtctccag tgagctggag aggcaagccc tggagaagca gggcagggaa
      721 gccgagaagg agatccagga catcaaccag cttccagaag aggccttgct gggaaacagg
      781 ctggacagcc acgactggga gaagatttcc aatattaact ttgaaggcag ccgcagtgca
      841 gaggagatcc ggaagttctg gcagaactcg gagcacccca gcatcaacaa gcaggagtgg
      901 agcagggagg aggaggagcg gctgcaggcg atcgcggctg cacacggcca cctggagtgg
      961 cagaagattg cagaggagct ggggaccagc cgcagcgcct tccagtgcct gcagaaattc
     1021 cagcagcaca acaaagctct gaaacgcaag gagtggacag aggaggagga ccgcatgctc
     1081 acgcagctgg tgcaggagat gcgcgtcggc agccacatcc cctaccgcag aattgtctac
     1141 tatatggaag ggagagactc catgcagctg atctaccgat ggaccaagag cttggatcct
     1201 ggtctgaaga agggttactg ggccccggag gaagatgcta agttgcttca agctgttgcc
     1261 aaatacgggg agcaggattg gtttaaaatc cgggaagagg tgccaggtag gagcgatgcc
     1321 cagtgccgag atcggtatct caggagatta catttcagct tgaaaaaggg tcggtggaat
     1381 ttaaaagaag aggaacagtt aattgaatta atagaaaaat atggtgtcgg tcactgggca
     1441 aaaatagctt ctgagctgcc ccatcggtct ggctcccagt gtctgagcaa gtggaagatc
     1501 atgatgggga agaagcaggg tctccggagg cggcggcgga gggcccgtca cagcgtccgg
     1561 tggagctcta ccagcagcag cggcagcagc agtggcagca gtggagggag cagcagcagc
     1621 agcagcagca gcagcgagga ggacgagcca gagcaggcgc aggccgggga gggtgacaga
     1681 gcgctgctgt ccccacagta catggtcccg gacatggacc tgtgggttcc tgccaggcag
     1741 agcaccagcc agccatggag aggaggggca ggggcctggc tgggaggccc cgctgcctcc
     1801 ctcagccctc ccaaggggtc cagtgccagc cagggcggca gcaaggaagc ttccaccaca
     1861 gccgcggctc ctggagagga gacgagtccg gtgcaggtcc ctgccagggc ccacggccct
     1921 gtcccgaggt ctgcccaggc ctcccactca gcagacactc gcccggcggg cgcagagaag
     1981 caggccctgg agggtgggag gcgtctgctg acagtgcctg tggagaccgt gctgagggtg
     2041 ctcagggcca acacggctgc tcggagctgc acacagaaag agcagctgag gcagccaccc
     2101 ctgcccacct catccccagg ggtcagctct ggtgacagcg tggcccgatc ccatgtgcag
     2161 tggctacggc acagagccac ccagagtggg cagcggcgct ggagacacgc tctgcaccgg
     2221 aggctcctga accgcaggct gctgctggct gtgacccctt gggtagggga cgttgtcgtg
     2281 ccctgcacac aggcttccca gagacccgcc gtagtgcaga ctcaagcgga tggcctcagg
     2341 gagcagctgc agcaggcccg cctggccagc acccctgtgt ttaccctgtt tacccagctg
     2401 ttccacatcg atactgccgg ctgcttggag gtcgtccgag agaggaaggc cctgccaccc
     2461 aggctgcccc aggctggtgc tcgggaccca ccagttcatc ttctgcaggc atcctcaagt
     2521 gcccagagca ccccaggcca cctcttccca aacgtgccgg ctcaagaagc ctcaaagagt
     2581 gccagccaca aagggagccg aagactggcg tccagccggg tggagcgcac cctaccccag
     2641 gcgtccctgc tggcttcaac cggcccccgg cccaagccca agactgtgtc ggagctgctt
     2701 caggagaagc ggcttcagga ggcccgtgcc agggaggcca cccggggccc ggtggtgctc
     2761 ccgtcccagc tgctggtctc ctcgtctgtg atcctccagc cccctctacc acacacccca
     2821 cacggccgcc cagccccggg tcccaccgtc ttaaatgtac cgctctctgg gcctggggcc
     2881 cccgcggcag ccaaacctgg cacttctggc tcctggcagg aggctgggac ttcagccaag
     2941 gacaagagac tctccaccat gcaagccctg cccctcgctc ctgtcttctc agaggccgag
     3001 ggcacagccc ctgctgcttc ccaagcccct gccctgggcc ccggccagat ctctgtgagc
     3061 tgccccgaga gtggtctcgg acagtctcag gcccccgctg catcccggaa gcagggcctg
     3121 cctgaggcgc caccctttct ccccgcagcc cccagcccca ccccactgcc cgtccagccc
     3181 ctcagcctga cgcacatagg agggccacat gtggcgacca gtgtccccct gcctgtcacc
     3241 tgggtgctca cagcccaggg gcttctccct gttcctgtac cagctgtggt gagccttccc
     3301 aggccagcag ggacccctgg ccccgcaggg ctgctggcca ctctgctgcc tcccctgact
     3361 gagactcggg cggcccaggg ccccagggcc ccagcgttga gcagctcttg gcagccccca
     3421 gccaatatga acagggaacc ggagccttcc tgcaggacag acaccccagc tcctcccaca
     3481 cacgccctct cccaaagtcc tgcagaagcg gatggcagtg tggcctttgt ccctggagag
     3541 gcccaggtgg ccagggagat acctgagccc aggacgtcct cccacgctga ccctcctgaa
     3601 gcagaacccc cttggtccgg gaggctgcca gccttcggtg gtgtcatccc agcaactgag
     3661 ccaaggggga cgccggggtc cccctcaggg acacaggagc ccagggggcc tctgggcctg
     3721 gagaagctgc ccctgcgcca gcctgggcct gagaaggggg ccctggacct ggagaagccg
     3781 cccctacccc agcctgggcc tgagaagggg gccctggacc tgggcctgct gtcccaggag
     3841 ggcgaggcgg ccacacagca gtggctgggg ggccagcggg gggtgcgtgt gcctcttctg
     3901 ggcagcagac tgccctatca gcccccagcc ctgtgcagcc tgcgagctct gtccggtctc
     3961 ctactccaca agaaggccct ggagcacaag gccacctccc tggtggtggg gggcgaggct
     4021 gagcggccgg ccggagcact gcaagcctca ctggggctgg tgcgggggca gctccaggac
     4081 aacccggcct acctcctgtt gcgggcgcgg ttcctggcag ccttcaccct ccctgcgctc
     4141 ctggccaccc tggcccccca aggcgtccgc accaccctct cagtaccttc gagggtgggc
     4201 tctgagagtg aggatgaaga cctcctgagt gagctggaac ttgcagacag ggacgggcag
     4261 ccgggctgca cgacagccac atgccccatt cagggagccc cagactctgg taaatgctct
     4321 gcttcctcct gcctggatac ttctaatgac cctgacgacc tggacgtgct cagaacccgg
     4381 catgccaggc acacccggaa gcggaggcgg ctggtgtagt aatgaactag tccgtaactt
     4441 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgt gtgtttacat
     4501 gcgactcatt t
//
