LOCUS       sequence-28514-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, 
            Yuan W, Piston DW, Magnuson MA
  TITLE     Quantification of factors influencing fluorescent protein expression
            using RMCE to generate an allelic series in the ROSA26 locus in 
            mice.
  JOURNAL   Dis Model Mech. 2011 Jul;4(4):537-47. doi: 10.1242/dmm.006569. Epub 
            2011 Feb 14.
  PUBMED    21324933
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.006569"; journalName: "Dis Model Mech"; date: 
            "2011-07"; volume: "4"; issue: "4"; pages: "537-47"
COMMENT     Sequence Label: pLCA.66/2272
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    157..381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     370..387
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(530..549)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cgcggggcgg ccgctctgat catatgccta ggacgcgttt cgaaggtacc tggatccata
       61 acttcgtata ggatacttta tacgaagtta tctgcaggaa ttcaagggtt ccgcaagctc
      121 tagtcgagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
      181 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
      241 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
      301 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
      361 gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctagagaat tgatcccctc
      421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
      481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
      541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
      601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
      661 cgagatcatc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
      721 gcccctgatg ctcttcgtca
//
