LOCUS       sequence-285150-        2006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2622
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacttc gaacaatccg cggaaattca gcgagaagat cgcgctgcac
       61 aatcagaagc aggcggagga gacggcggcc ttcgaggagg tcatgaagga cctgagcctg
      121 acgcgggccg cgcggctcca gctccagaaa tcccagtacc tgcaactggg ccccagccga
      181 ggccagtact atggcgggtc cctgcccaac gtgaaccaga tcgggagtgg caccatggac
      241 ctgcccttcc agaccccctt ccaatcctcg ggcctggaca ccagccggac cacccggcac
      301 catgggctgg tggacagggt gtaccgggag cgtggccggc tcggctcccc acaccgccgg
      361 cccctgtcag tggacaaaca cggacggcag gccgacagct gcccctatgg caccatgtac
      421 ctctcaccac ccgcggacac cagctggaga aggaccaatt ctgactccgc cctgcaccag
      481 agcacaatga cgcccacgca gccagaatcc tttagcagtg ggtcccagga cgtgcaccag
      541 aaaagagtct tactgttaac agtcccagga atggaagaga ccacatcaga ggcagacaaa
      601 aacctttcca agcaagcatg ggacaccaag aagacggggt ccaggcccaa gtcctgtgag
      661 gtccccggaa tcaacatctt cccgtctgcc gaccaggaaa acactacagc cctgatcccc
      721 gccacccaca acacaggggg gtccctgccc gacctgacca acatccactt cccctccccg
      781 ctcccgaccc cgctggaccc cgaggagccc accttccctg cactgagcag ctccagcagc
      841 accggcaacc tcgcggccaa cctgacgcac ctgggcatcg gtggcgccgg ccagggaatg
      901 agcacacctg gctcctctcc acagcaccgc ccagctggcg tcagccccct gtccctgagc
      961 acagaggcaa ggcgtcagca ggcatcgccc accctgtccc cgctgtcacc catcactcag
     1021 gctgtagcca tggacgccct gtctctggag cagcagctgc cctacgcctt cttcacccag
     1081 gcgggctccc agcagccacc gccgcagccc cagcccccgc cgcctcctcc acccgcgtcc
     1141 cagcagccac cacccccgcc acccccacag gcgcccgtcc gcctgccccc tggtggcccc
     1201 ctgttgccca gcgccagcct gactcgtggg ccacagccgc ccccgcttgc agtcacggta
     1261 ccgtcctctc tcccccagtc ccccccagag aaccctggcc agccatcgat ggggatcgac
     1321 atcgcctcgg cgccggctct gcagcagtac cgcactagcg ccggctcccc ggccaaccag
     1381 tctcccacct cgccagtctc caatcaaggc ttctccccag ggagctcccc gcaacacact
     1441 tccaccctgg gcagcgtgtt tggggacgcg tactatgagc agcagatggc ggccaggcag
     1501 gccaatgctc tgtcccacca gctggagcag ttcaacatga tggagaacgc catcagctcc
     1561 agcagcctgt acagcccggg ctccacactc aactactcgc aggcggccat gatgggcctc
     1621 acgggcagcc acgggagcct gccggactcg cagcaactgg gatacgccag ccacagtggc
     1681 atccccaaca tcatcctcac agtgacagga gagtcccccc ccagcctctc taaagaactg
     1741 accagctctc tggccggggt cggcgacgtc agcttcgact ccgacagcca gtttcccctg
     1801 gacgaactca agatcgaccc cctgaccctc gacggactgc acatgctcaa cgaccccgac
     1861 atggttctgg ccgacccagc caccgaggac accttccgga tggaccgcct gtagtaatga
     1921 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1981 gataagcgaa agtgtgtctt aatgca
//
