LOCUS       sequence-285171-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2755
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacccagt ggcttttaag gatgtggctg tgaacttcac ccaggaggag
       61 tgggctttgc tggatatttc ccagaggaaa ctctacaggg aagtgatgct ggaaactttc
      121 aggaacctga cctctttagg aaaaaggtgg aaagaccaga acattgaata tgagcaccaa
      181 aaccccagga gaaacttcag gagtctcata gaagaaaaag tcaatgaaat taaagatgac
      241 agtcattgtg gagaaacttt taccccagtt ccagatgaca gactgaactt ccaggagaag
      301 aaagcttctc ctgaagtaaa atcatgtgaa agctttgtgt gtggagaagt tggcctaggt
      361 aactcatctt ttaatatgaa catcagaggt gacattggac acaaggcata tgagtatcag
      421 gaatatggac caaagccatg taagtgtcaa caacctaaaa aagccttcag ataccgcccc
      481 tcctttagaa cacaagaaag ggatcacact ggagagaaac ccaatgcttg taaagtatgt
      541 ggaaaaacct ttatttccca ttcaagtgtt cgaagacaca tggtaatgca cagtggggat
      601 ggaccttata aatgtaagtt ttgtgggaaa gcattccatt gtctcagatt atatcttatc
      661 catgaaagaa ttcacactgg agagaaacca tgtgaatgta aacagtgtgg taaatccttt
      721 agttattctg ctacccatcg aatacataaa agaactcaca ctggagaaaa gccttatgaa
      781 tatcaggagt gtgggaaagc atttcatagt cccagatcct atcgtagaca tgaaaggatt
      841 cacatgggag aaaaggctta tcaatgtaag gaatgtggaa aagcattcac gtgtccccgt
      901 tatgttcgta tacatgaaag gacccactct aggaaaaatc tctatgaatg taagcagtgt
      961 gggaaagcat tatcctctct tacaagtttt caaacacacg taagattgca ctctggagaa
     1021 agaccttatg aatgtaagat atgtggaaaa gacttttgtt ctgtgaattc atttcaaaga
     1081 catgaaaaaa ttcacagtgg agagaaaccc tataaatgta agcagtgtgg taaagccttc
     1141 cctcattcca gttcccttcg atatcatgaa aggactcaca ctggagagaa accctatgag
     1201 tgtaagcaat gtgggaaagc cttcagatct gcctcacacc ttcgagtgca tggtaggact
     1261 cacactggag agaaaccgta tgaatgtaag gaatgtggga aagccttcag atatgtgaat
     1321 aaccttcaaa gtcatgaaag gacacaaaca cacataagaa tacactctgg agaaagacgt
     1381 tataaatgta agatatgtgg gaaaggcttt tattgtccca aatcatttca aagacatgaa
     1441 aaaactcaca ctggagagaa actctatgaa tgcaagcaac gttcagtagt tccttcagta
     1501 gttccagttc cttttgatat catgaaagga ctcacactgg agagaagccc tataaatgcg
     1561 agcaatgtgg gaaagccttc agagctgtgt caatcctttg aatgcatggt aggactcacc
     1621 ctgaagagaa accctatgag tgtgagcaat gacggaaagc cttcagatct gccccacacc
     1681 tttgaatacg tggtaggaca cacaatggag agaagcccta tgcatgtaag gaatgtggga
     1741 aacccttcgg atctgcccag aaccttcgaa ttcatgaaag gacacaaaca cacatagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgatgcat atcacccact acaaacgag
//
